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Conserved domains on  [gi|2529040574|gb|WJP96689|]
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glycosyltransferase family 2 protein [Klebsiella variicola]

Protein Classification

glycosyltransferase family 2 protein( domain architecture ID 10118426)

glycosyltransferase family 2 protein catalyzes the transfer of saccharide moieties from a donor to an acceptor to form glycosidic bonds

CAZY:  GT2
EC:  2.4.-.-
Gene Ontology:  GO:0016757
PubMed:  9445404|12691742
SCOP:  3000077

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 2-209 4.53e-58

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


:

Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 184.03  E-value: 4.53e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLLKVADECIVVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALK 81
Cdd:cd02511    17 RCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDADERLTPELADEILALL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  82 AGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSVRLPGFVRHDTFYSLHE 160
Cdd:cd02511    97 ATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGIVLKGDILHYGYKSLEE 171

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2529040574 161 VFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 209
Cdd:cd02511   172 FLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 2-209 4.53e-58

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 184.03  E-value: 4.53e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLLKVADECIVVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALK 81
Cdd:cd02511    17 RCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDADERLTPELADEILALL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  82 AGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSVRLPGFVRHDTFYSLHE 160
Cdd:cd02511    97 ATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGIVLKGDILHYGYKSLEE 171

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2529040574 161 VFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 209
Cdd:cd02511   172 FLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 13-124 2.23e-16

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 75.12  E-value: 2.23e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  13 ECIVVDSGSTDETVSICQQFG-----LTVHHHAYKA-HGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALKAGEEP 86
Cdd:COG0463    33 EIIVVDDGSTDGTAEILRELAakdprIRVIRLERNRgKGAARNAGLAAARGDYIAFLDADDQLDPEKLEELVAALEEGPA 112

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2529040574  87 DPTCAWR--------LPRYWFVLGTQVRTIYPISSPDYPVRLFNRQ 124
Cdd:COG0463   113 DLVYGSRliregesdLRRLGSRLFNLVRLLTNLPDSTSGFRLFRRE 158
glyco_like_mftF TIGR04283
transferase 2, rSAM/selenodomain-associated; This enzyme may transfer a nucleotide, or it ...
 2-80 7.65e-12

transferase 2, rSAM/selenodomain-associated; This enzyme may transfer a nucleotide, or it sugar moiety, as part of a biosynthetic pathway. Other proposed members of the pathway include another transferase (TIGR04282), a phosphoesterase, and a radical SAM enzyme (TIGR04167) whose C-terminal domain (pfam12345) frequently contains a selenocysteine. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 275103 [Multi-domain]  Cd Length: 220  Bit Score: 62.53  E-value: 7.65e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLL------KVADECIVVDSGSTDETVSICQQFGLTVHHHAyKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVA 75
Cdd:TIGR04283  12 ATLPELLadlqalRGDAEVIVVDGGSTDGTVEIARSLGAKVIHSP-KGRARQMNAGAALAKGDILLFLHADTRLPKDFLE 90


                  ....*
gi 2529040574  76 AIQAL 80
Cdd:TIGR04283  91 AIRRA 95
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 12-125 9.72e-12

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 61.26  E-value: 9.72e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQF-----GLTVHHHAYK-AHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALKAGEE 85
Cdd:pfam00535  28 FEIIVVDDGSTDGTVEIAEEYakkdpRVRVIRLPENrGKAGARNAGLRAATGDYIAFLDADDEVPPDWLEKLVEALEEDG 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2529040574  86 PDPTCAWRLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQ 125
Cdd:pfam00535 108 ADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGL 147
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 2-38 8.01e-04

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 39.90  E-value: 8.01e-04
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 2529040574   2 EVLPPLLkvaDECIVVDSGSTDETVSICQQFGLTVHH 38
Cdd:PRK13915   56 LLMEPLV---DELIVIDSGSTDATAERAAAAGARVVS 89
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 2-209 4.53e-58

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 184.03  E-value: 4.53e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLLKVADECIVVDSGSTDETVSICQQFGLTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALK 81
Cdd:cd02511    17 RCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQRWWDGFGAQRNFALELATNDWVLSLDADERLTPELADEILALL 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  82 AGEEPDptcAWRLPRYWFVLGTQVRtiYPISSPDYPVRLFNRQQARFNDRPVDDQVVGHA-RSVRLPGFVRHDTFYSLHE 160
Cdd:cd02511    97 ATDDYD---GYYVPRRNFFLGRWIR--HGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgVGIVLKGDILHYGYKSLEE 171

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2529040574 161 VFNKLNSYTTRLVKYQQIK---PSLTRGIVSA--IGAFFKWYLFSGAWRYGKVG 209
Cdd:cd02511   172 FLEKHNRYSSLEAKDLAAKgkkRSLLKGLLLGrpLLAFLKMYILKRGFLDGRAG 225
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 13-124 2.23e-16

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 75.12  E-value: 2.23e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  13 ECIVVDSGSTDETVSICQQFG-----LTVHHHAYKA-HGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALKAGEEP 86
Cdd:COG0463    33 EIIVVDDGSTDGTAEILRELAakdprIRVIRLERNRgKGAARNAGLAAARGDYIAFLDADDQLDPEKLEELVAALEEGPA 112

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2529040574  87 DPTCAWR--------LPRYWFVLGTQVRTIYPISSPDYPVRLFNRQ 124
Cdd:COG0463   113 DLVYGSRliregesdLRRLGSRLFNLVRLLTNLPDSTSGFRLFRRE 158
glyco_like_mftF TIGR04283
transferase 2, rSAM/selenodomain-associated; This enzyme may transfer a nucleotide, or it ...
 2-80 7.65e-12

transferase 2, rSAM/selenodomain-associated; This enzyme may transfer a nucleotide, or it sugar moiety, as part of a biosynthetic pathway. Other proposed members of the pathway include another transferase (TIGR04282), a phosphoesterase, and a radical SAM enzyme (TIGR04167) whose C-terminal domain (pfam12345) frequently contains a selenocysteine. [Unknown function, Enzymes of unknown specificity]


Pssm-ID: 275103 [Multi-domain]  Cd Length: 220  Bit Score: 62.53  E-value: 7.65e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLL------KVADECIVVDSGSTDETVSICQQFGLTVHHHAyKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVA 75
Cdd:TIGR04283  12 ATLPELLadlqalRGDAEVIVVDGGSTDGTVEIARSLGAKVIHSP-KGRARQMNAGAALAKGDILLFLHADTRLPKDFLE 90


                  ....*
gi 2529040574  76 AIQAL 80
Cdd:TIGR04283  91 AIRRA 95
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 12-125 9.72e-12

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 61.26  E-value: 9.72e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQF-----GLTVHHHAYK-AHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAIQALKAGEE 85
Cdd:pfam00535  28 FEIIVVDDGSTDGTVEIAEEYakkdpRVRVIRLPENrGKAGARNAGLRAATGDYIAFLDADDEVPPDWLEKLVEALEEDG 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2529040574  86 PDPTCAWRLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQ 125
Cdd:pfam00535 108 ADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGL 147
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 13-83 7.65e-10

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 57.83  E-value: 7.65e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  13 ECIVVDSGSTDETVSICQQFG-----LTVHHHAYKAH-GAQMNYAIGLASHDWVLCMDSDEILDND----VVAAIQALKA 82
Cdd:COG1215    62 EVIVVDDGSTDETAEIARELAaeyprVRVIERPENGGkAAALNAGLKAARGDIVVFLDADTVLDPDwlrrLVAAFADPGV 141


                  .
gi 2529040574  83 G 83
Cdd:COG1215   142 G 142
Glyco_tranf_GTA_type cd00761
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 12-99 1.55e-09

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


Pssm-ID: 132997 [Multi-domain]  Cd Length: 156  Bit Score: 55.20  E-value: 1.55e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQFGLT------VHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAiQALKAGEE 85
Cdd:cd00761    27 FEVIVVDDGSTDGTLEILEEYAKKdprvirVINEENQGLAAARNAGLKAARGEYILFLDADDLLLPDWLER-LVAELLAD 105

                          90
                  ....*....|....
gi 2529040574  86 PDPTCAWRLPRYWF 99
Cdd:cd00761   106 PEADAVGGPGNLLF 119
CESA_like cd06423
CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily ...
 12-88 1.64e-08

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.


Pssm-ID: 133045 [Multi-domain]  Cd Length: 180  Bit Score: 52.61  E-value: 1.64e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQFG-------LTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDvvaAIQALKAGE 84
Cdd:cd06423    27 LEVIVVDDGSTDDTLEILEELAalyirrvLVVRDKENGGKAGALNAGLRHAKGDIVVVLDADTILEPD---ALKRLVVPF 103


                  ....
gi 2529040574  85 EPDP 88
Cdd:cd06423   104 FADP 107
WcaE COG1216
Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];
10-77 2.17e-08

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];


Pssm-ID: 440829 [Multi-domain]  Cd Length: 202  Bit Score: 52.69  E-value: 2.17e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2529040574  10 VADECIVVDSGSTDETVSICQQF---GLTVHHHAYKA-HGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAI 77
Cdd:COG1216    31 PPFEVIVVDNGSTDGTAELLAALafpRVRVIRNPENLgFAAARNLGLRAAGGDYLLFLDDDTVVEPDWLERL 102
GT_2_WfgS_like cd06433
WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella ...
 13-128 3.02e-08

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133055 [Multi-domain]  Cd Length: 202  Bit Score: 52.16  E-value: 3.02e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  13 ECIVVDSGSTDETVSICQQFGLTVHH----------HAykahgaqMNYAIGLASHDWVLCMDSDEIL-DNDVVAAIQALK 81
Cdd:cd06433    29 EYIVIDGGSTDGTVDIIKKYEDKITYwisepdkgiyDA-------MNKGIALATGDIIGFLNSDDTLlPGALLAVVAAFA 101

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 2529040574  82 AGEEPDPTCAwrlPRYWFVLGTQVRTIYPISSPDYPVRLFNR---QQARF 128
Cdd:cd06433   102 EHPEVDVVYG---DVLLVDENGRVIGRRRPPPFLDKFLLYGMpicHQATF 148
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 12-127 5.60e-08

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 51.42  E-value: 5.60e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQFGLTVHHHAYKAH------GAQMNYAIGLASHDWVLCMDSD------EIL---------D 70
Cdd:cd04179    29 YEIIVVDDGSTDGTAEIARELAARVPRVRVIRLsrnfgkGAAVRAGFKAARGDIVVTMDADlqhppeDIPkllekllegG 108

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2529040574  71 NDVVAAIQALKAGEEPDPtcaW--RLPRYWFVLGTQVRTIYPISSPDYPVRLFNRQQAR 127
Cdd:cd04179   109 ADVVIGSRFVRGGGAGMP---LlrRLGSRLFNFLIRLLLGVRISDTQSGFRLFRREVLE 164
GT_2_like_a cd02522
GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; ...
 2-77 1.93e-07

GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; Glycosyltransferase family 2 (GT-2) subfamily of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133013 [Multi-domain]  Cd Length: 221  Bit Score: 50.26  E-value: 1.93e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2529040574   2 EVLPPLLKVADECIVVDSGSTDETVSICQQFGLTVHHHAyKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAI 77
Cdd:cd02522    19 ASLRRLNPLPLEIIVVDGGSTDGTVAIARSAGVVVISSP-KGRARQMNAGAAAARGDWLLFLHADTRLPPDWDAAI 93
Succinoglycan_BP_ExoA cd02525
ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA ...
 9-77 4.08e-07

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.


Pssm-ID: 133016 [Multi-domain]  Cd Length: 249  Bit Score: 49.54  E-value: 4.08e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2529040574   9 KVADECIVVDSGSTDETVSICQQFG-----LTVHHHAYKAHGAQMNYAIGLASHDWVLCMDSDEILDNDVVAAI 77
Cdd:cd02525    29 KDLIEIIVVDGGSTDGTREIVQEYAakdprIRLIDNPKRIQSAGLNIGIRNSRGDIIIRVDAHAVYPKDYILEL 102
CESA_like_1 cd06439
CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of ...
 13-82 4.05e-05

CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of cellulose synthase (CESA) superfamily. CESA superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members of the superfamily include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins.


Pssm-ID: 133061 [Multi-domain]  Cd Length: 251  Bit Score: 43.73  E-value: 4.05e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2529040574  13 ECIVVDSGSTDETVSICQQF---GLTVHH---HAYKAHGaqMNYAIGLASHDWVLCMDSDEILDNDvvaAIQALKA 82
Cdd:cd06439    62 EIIVVSDGSTDGTAEIAREYadkGVKLLRfpeRRGKAAA--LNRALALATGEIVVFTDANALLDPD---ALRLLVR 132
Glyco_tranf_2_4 pfam13704
Glycosyl transferase family 2; Members of this family of prokaryotic proteins include putative ...
 11-72 9.36e-05

Glycosyl transferase family 2; Members of this family of prokaryotic proteins include putative glucosyltransferases,


Pssm-ID: 433416 [Multi-domain]  Cd Length: 97  Bit Score: 40.31  E-value: 9.36e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2529040574  11 ADECIVVDSGSTDETVSICQQ---FGLTVHHHAYKAHGAQMNYAIGLASH----DWVLCMDSDEILDND 72
Cdd:pfam13704  19 FDHIYVYDNGSDDGTAEILARlpdVSILRSDLSYKDARFQVDWRNALLARyaeaDWVLVVDADEFLVYP 87
GT_2_like_d cd04196
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 12-87 2.62e-04

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133039 [Multi-domain]  Cd Length: 214  Bit Score: 41.08  E-value: 2.62e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQFGLTVHHHAY-----KAHGAQMNY--AIGLASHDWVLCMDSDEI-LDNDVVAAIQALKAG 83
Cdd:cd04196    28 DELIISDDGSTDGTVEIIKEYIDKDPFIIIlirngKNLGVARNFesLLQAADGDYVFFCDQDDIwLPDKLERLLKAFLKD 107


                  ....
gi 2529040574  84 EEPD 87
Cdd:cd04196   108 DKPL 111
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 2-38 8.01e-04

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 39.90  E-value: 8.01e-04
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 2529040574   2 EVLPPLLkvaDECIVVDSGSTDETVSICQQFGLTVHH 38
Cdd:PRK13915   56 LLMEPLV---DELIVIDSGSTDATAERAAAAGARVVS 89
GT_2_like_c cd04186
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 15-87 1.69e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133029 [Multi-domain]  Cd Length: 166  Bit Score: 37.92  E-value: 1.69e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2529040574  15 IVVDSGSTDETVSICQQFGLTVHHHAYKAH---GAQMNYAIGLASHDWVLCMDSDEILDNDVVAAiqALKAGEEPD 87
Cdd:cd04186    30 IVVDNASTDGSVELLRELFPEVRLIRNGENlgfGAGNNQGIREAKGDYVLLLNPDTVVEPGALLE--LLDAAEQDP 103
GT_2_like_e cd04192
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 13-66 3.54e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133035 [Multi-domain]  Cd Length: 229  Bit Score: 37.65  E-value: 3.54e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2529040574  13 ECIVVDSGSTDETVSICQQFGLTVHHH-----------AYKAHGAQMnyAIGLASHDWVLCMDSD 66
Cdd:cd04192    30 EVILVDDHSTDGTVQILEFAAAKPNFQlkilnnsrvsiSGKKNALTT--AIKAAKGDWIVTTDAD 92
GT_2_like_b cd04185
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 12-90 7.70e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133028 [Multi-domain]  Cd Length: 202  Bit Score: 36.46  E-value: 7.70e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574  12 DECIVVDSGSTDETVSICQQFG----LTVHHHAYKAHGA-------QMNYAIGlasHDWVLCMDSDEILDNDVVAAIqaL 80
Cdd:cd04185    27 DHIIVIDNASTDGTAEWLTSLGdldnIVYLRLPENLGGAggfyegvRRAYELG---YDWIWLMDDDAIPDPDALEKL--L 101

                          90
                  ....*....|
gi 2529040574  81 KAGEEPDPTC 90
Cdd:cd04185   102 AYADKDNPQF 111
DPM1_like_bac cd04187
Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes ...
 2-79 8.69e-03

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133030 [Multi-domain]  Cd Length: 181  Bit Score: 35.92  E-value: 8.69e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2529040574   2 EVLPPLL----KVAD------ECIVVDSGSTDETVSICQQ----------------FGltvhHHAykAHGAQMNYAIGla 55
Cdd:cd04187    10 ENLPELYerlkAVLEslgydyEIIFVDDGSTDRTLEILRElaardprvkvirlsrnFG----QQA--ALLAGLDHARG-- 81

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 2529040574  56 shDWVLCMDSD------EIL--------DNDVVAAIQA 79
Cdd:cd04187    82 --DAVITMDADlqdppeLIPemlakweeGYDVVYGVRK 117
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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