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Conserved domains on  [gi|1125431675|gb|OLD60677|]
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hypothetical protein AUF60_00100 [Gemmatimonadetes bacterium 13_1_20CM_69_28]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 10007626)

nuclear transport factor 2 (NTF2) family protein with a SnoaL-like polyketide cyclase domain

CATH:  3.10.450.50
SCOP:  4001306|3000472

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
6-116 1.71e-22

Ketosteroid isomerase-related protein [General function prediction only];


:

Pssm-ID: 442849  Cd Length: 126  Bit Score: 84.98  E-value: 1.71e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   6 RQLLDSFGRGWEAGDVDGIVSVFAPDAVFLE--TPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAGPWFATEFRC 83
Cdd:COG3631     3 RELVRRFYAAFNAGDVDALLALLAEDVVWEDpgGPGAGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGTV 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1125431675  84 TYRRRRTGEWVDARGAMFCETKDGKISEMRMYW 116
Cdd:COG3631    83 RGTVLATGKPYENRYADVFTVRDGKIVRVREYF 115
 
Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
6-116 1.71e-22

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 84.98  E-value: 1.71e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   6 RQLLDSFGRGWEAGDVDGIVSVFAPDAVFLE--TPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAGPWFATEFRC 83
Cdd:COG3631     3 RELVRRFYAAFNAGDVDALLALLAEDVVWEDpgGPGAGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGTV 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1125431675  84 TYRRRRTGEWVDARGAMFCETKDGKISEMRMYW 116
Cdd:COG3631    83 RGTVLATGKPYENRYADVFTVRDGKIVRVREYF 115
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 14-109 2.15e-16

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 68.85  E-value: 2.15e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675  14 RGWEAGDVDGIVSVFAPDAVFlETPFSEQsVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAGPWFATEFRCTYRRRRTGEW 93
Cdd:pfam12680   6 EALNAGDLDALAALFAPDAVF-HDPGGPL-RGRDAIRAFFAALFAAFPDLRFEIHDVIADGDRVAVRWTVTGTIPPTGRG 83

                          90
                  ....*....|....*.
gi 1125431675  94 VDARGAMFCETKDGKI 109
Cdd:pfam12680  84 VRVRGVDVFRFRDGKI 99
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 1-116 2.20e-06

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 43.18  E-value: 2.20e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   1 MNAAYRQLLDSFgrgwEAGDVDGIVSVFAPDAVfLETPF-SEQSVGSDAIRAYWKDIPVNQAEVTFkAGEIYA-AGPWFA 78
Cdd:cd00781     5 MKAAVQRYVEAV----NAGDPEGIVALFADDAT-VEDPVgSPPRSGRAAIAAFYAQSLGGAKRLEL-TGPVRAsHGGEAA 78

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1125431675  79 TEFRCTYRRRRTGEWVDARGAM-FCEtkDGKISEMRMYW 116
Cdd:cd00781    79 FAFRVEFEWEGQPCVVRVIDVMrFDA--DGRIVSMRAYW 115
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
3-112 2.56e-05

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 41.85  E-value: 2.56e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   3 AAYRQLLDSFGRGWEAGDVDGIVSVFAPDAVFLETPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAG-PWFAtef 81
Cdd:PRK08241  214 PEERALLARYVAAFEAYDVDALVALLTEDATWSMPPFPLWYRGRDAIAAFLAGQCPGAGCGGSRLVPTRANGqPAFA--- 290

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1125431675  82 rcTYRRRRTGEWVDARGAMFCETKDGKISEM 112
Cdd:PRK08241  291 --QYMRDPDGGGHRPWALHVLELRGGRIAHV 319
TIGR02246 TIGR02246
conserved hypothetical protein; This family consists of uncharacterized proteins found in a ...
 4-52 4.35e-05

conserved hypothetical protein; This family consists of uncharacterized proteins found in a number of genera and species, including Streptomyces, Xanthomonas, Oceanobacillus iheyensis, Caulobacter crescentus CB15, and Xylella fastidiosa. The function is unknown.


Pssm-ID: 274053  Cd Length: 128  Bit Score: 40.05  E-value: 4.35e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1125431675   4 AYRQLLDSFGRGWEAGDVDGIVSVFAPDAVFLETPFSEQSvGSDAIRAY 52
Cdd:TIGR02246   5 AIRALVARWEAAWAAGDAEAFADLFAPDGVFVTVPGQVRK-GREAIRAA 52
 
Name Accession Description Interval E-value
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
6-116 1.71e-22

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 84.98  E-value: 1.71e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   6 RQLLDSFGRGWEAGDVDGIVSVFAPDAVFLE--TPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAGPWFATEFRC 83
Cdd:COG3631     3 RELVRRFYAAFNAGDVDALLALLAEDVVWEDpgGPGAGTYRGKEAVRAFFARLAAAFEDLRFEVRRVVADGDRVVVEGTV 82

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1125431675  84 TYRRRRTGEWVDARGAMFCETKDGKISEMRMYW 116
Cdd:COG3631    83 RGTVLATGKPYENRYADVFTVRDGKIVRVREYF 115
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 14-109 2.15e-16

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 68.85  E-value: 2.15e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675  14 RGWEAGDVDGIVSVFAPDAVFlETPFSEQsVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAGPWFATEFRCTYRRRRTGEW 93
Cdd:pfam12680   6 EALNAGDLDALAALFAPDAVF-HDPGGPL-RGRDAIRAFFAALFAAFPDLRFEIHDVIADGDRVAVRWTVTGTIPPTGRG 83

                          90
                  ....*....|....*.
gi 1125431675  94 VDARGAMFCETKDGKI 109
Cdd:pfam12680  84 VRVRGVDVFRFRDGKI 99
YybH COG4319
Ketosteroid isomerase homolog YybH [General function prediction only];
3-93 4.96e-09

Ketosteroid isomerase homolog YybH [General function prediction only];


Pssm-ID: 443460 [Multi-domain]  Cd Length: 131  Bit Score: 50.52  E-value: 4.96e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   3 AAYRQLLDSFGRGWEAGDVDGIVSVFAPDAVFLeTPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIY----------- 71
Cdd:COG4319     9 AAIRALLAAFAEAFNAGDADALAALYAEDAVFF-DPGGPPVRGREAIRAAWAAAFAAGPRVTFEVEDVRvlvsgdvavvt 87

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1125431675  72 ---------AAGPWFATEFRCT--YRRRRTGEW 93
Cdd:COG4319    88 grwrltgtdPDGEPVELAGRYTlvFRKQADGRW 120
COG5485 COG5485
Predicted ester cyclase [General function prediction only];
4-116 1.71e-06

Predicted ester cyclase [General function prediction only];


Pssm-ID: 444236 [Multi-domain]  Cd Length: 130  Bit Score: 43.69  E-value: 1.71e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   4 AYRQLLDSFgrgWEAGDVDGIVSVFAPDAVFlETPFSEQSVGSDAIRAYWKD----IPvnqaEVTFKAGEIYAAGPWFAT 79
Cdd:COG5485     1 LVRRFFEEA---WNEGDLDALDELVAPDFVY-HGPLPGEPRGPEGFRAFLRGlraaFP----DLRFEIEDLIAEGDRVAV 72

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1125431675  80 E--FRCTYrrrrTGEW---------VDARGAMFCETKDGKISEMRMYW 116
Cdd:COG5485    73 RwtFTGTH----TGEFlgipptgkrVEFRGIDIYRFEDGKIVEEWVVE 116
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 1-116 2.20e-06

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 43.18  E-value: 2.20e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   1 MNAAYRQLLDSFgrgwEAGDVDGIVSVFAPDAVfLETPF-SEQSVGSDAIRAYWKDIPVNQAEVTFkAGEIYA-AGPWFA 78
Cdd:cd00781     5 MKAAVQRYVEAV----NAGDPEGIVALFADDAT-VEDPVgSPPRSGRAAIAAFYAQSLGGAKRLEL-TGPVRAsHGGEAA 78

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1125431675  79 TEFRCTYRRRRTGEWVDARGAM-FCEtkDGKISEMRMYW 116
Cdd:cd00781    79 FAFRVEFEWEGQPCVVRVIDVMrFDA--DGRIVSMRAYW 115
SnoaL pfam07366
SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in ...
 4-117 1.60e-05

SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in nogalamycin biosynthesis. This family was formerly known as DUF1486. The proteins in this family adopt a distorted alpha-beta barrel fold. Structural data together with site-directed mutagenesis experiments have shown that SnoaL has a different mechanism to that of the classical aldolase for catalysing intramolecular aldol condensation.


Pssm-ID: 399975  Cd Length: 126  Bit Score: 41.13  E-value: 1.60e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   4 AYRQLLDSfgrgWEAGDVDGIVSVFAPDaVFLETPFSEQSVGSDAIRAY----WKDIPVNQAEVTfkagEIYAAGPWFAT 79
Cdd:pfam07366   3 VLRFYDEV----WNTGDLDALDEFVAED-VVDHNPPPGVARGLDGFRAFaemlFRAFPDLRFEVE----DLVAEGDKVAA 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 1125431675  80 efRCTYRRRRTGEW---------VDARGAMFCETKDGKISEmrmYWH 117
Cdd:pfam07366  74 --RLTFTGTHTGEFlgipptgrkVRFRGIDLYRFEDGKIVE---HWD 115
PRK08241 PRK08241
RNA polymerase subunit sigma-70;
3-112 2.56e-05

RNA polymerase subunit sigma-70;


Pssm-ID: 236196 [Multi-domain]  Cd Length: 339  Bit Score: 41.85  E-value: 2.56e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   3 AAYRQLLDSFGRGWEAGDVDGIVSVFAPDAVFLETPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGEIYAAG-PWFAtef 81
Cdd:PRK08241  214 PEERALLARYVAAFEAYDVDALVALLTEDATWSMPPFPLWYRGRDAIAAFLAGQCPGAGCGGSRLVPTRANGqPAFA--- 290

                          90       100       110
                  ....*....|....*....|....*....|.
gi 1125431675  82 rcTYRRRRTGEWVDARGAMFCETKDGKISEM 112
Cdd:PRK08241  291 --QYMRDPDGGGHRPWALHVLELRGGRIAHV 319
TIGR02246 TIGR02246
conserved hypothetical protein; This family consists of uncharacterized proteins found in a ...
 4-52 4.35e-05

conserved hypothetical protein; This family consists of uncharacterized proteins found in a number of genera and species, including Streptomyces, Xanthomonas, Oceanobacillus iheyensis, Caulobacter crescentus CB15, and Xylella fastidiosa. The function is unknown.


Pssm-ID: 274053  Cd Length: 128  Bit Score: 40.05  E-value: 4.35e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1125431675   4 AYRQLLDSFGRGWEAGDVDGIVSVFAPDAVFLETPFSEQSvGSDAIRAY 52
Cdd:TIGR02246   5 AIRALVARWEAAWAAGDAEAFADLFAPDGVFVTVPGQVRK-GREAIRAA 52
CaMKIID COG4875
Protein kinase association domain CaMKII_AD, NTF2-like superfamily [Posttranslational ...
 3-94 7.82e-05

Protein kinase association domain CaMKII_AD, NTF2-like superfamily [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 443903  Cd Length: 156  Bit Score: 39.61  E-value: 7.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   3 AAYRQLLDSfgrgWEA----GDVDGIVSVFAPDAVFLETPFSEQSVGSDAIRAYWKD-------IPVNQAEVTFKAGEIY 71
Cdd:COG4875    36 AQIAALFDR----WNAalqtGDADKVLALYAPDAVLLPTVSNKVRTTRAEIRDYFEHflakkpqGVIDERTIKIGCNTAV 111

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1125431675  72 AAGPW-FATE-----------FRCTYrRRRTGEWV 94
Cdd:COG4875   112 DSGTYtFTFTkdgstltvparYTFTY-EKQGGQWL 145
SnoaL_3 pfam13474
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 6-81 4.63e-04

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433238  Cd Length: 121  Bit Score: 36.95  E-value: 4.63e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431675   6 RQLLDSFGRGWEAGDVDGIVSVFAPDAVFLETPFSEQSVGSDAIRAYWKDIPVNQAEVTFKAGE--IYAAGP--WFATEF 81
Cdd:pfam13474   2 NALNDAWYEAAAEGDFDALFALWSDDISCIGTGAGELWRGREEFRRSWKQDFAQAQGWQFELLDrrVTVLGDvaWVTELL 81
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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