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Conserved domains on  [gi|1125431534|gb|OLD60559|]
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hypothetical protein AUF60_00650 [Gemmatimonadetes bacterium 13_1_20CM_69_28]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 106779)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdoMet_MTases super family cl17173
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 21-154 3.24e-31

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


The actual alignment was detected with superfamily member TIGR01444:

Pssm-ID: 473071  Cd Length: 143  Bit Score: 111.63  E-value: 3.24e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  21 VVADVGAHHGYFSVLMAhWVGARGKVYAFECLPENADIASRNVALNGLQNIAVVRTAVGAASGTIDIVDNSGGILGDRVP 100
Cdd:TIGR01444   1 VVIDVGANIGDTSLYFA-RKGAEGRVIAFEPLPDAYEILEENVKLNNLPNVVLLNAAVGDRDGELEFNVSDDDTGNSSLL 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1125431534 101 GMRVL------QVAMTSLDEFFGER---LPNLLKIDVEGYEFDVLTGARRCL-AARPKIALEFH 154
Cdd:TIGR01444  80 PTPDAdreseiEVEVVTLDDLVEEFgldKVDLLKIDVEGAELEVLRGAAETLlEKRPVIVLEVH 143
 
Name Accession Description Interval E-value
fkbM_fam TIGR01444
methyltransferase, FkbM family; Members of this family are characterized by two well-conserved ...
 21-154 3.24e-31

methyltransferase, FkbM family; Members of this family are characterized by two well-conserved short regions separated by a variable in both sequence and length. The first of the two regions is found in a large number of proteins outside this subfamily, a number of which have been characterized as methyltransferases. One member of the present family, FkbM, was shown to be required for a specific methylation in the biosynthesis of the immunosuppressant FK506 in Streptomyces strain MA6548.


Pssm-ID: 273628  Cd Length: 143  Bit Score: 111.63  E-value: 3.24e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  21 VVADVGAHHGYFSVLMAhWVGARGKVYAFECLPENADIASRNVALNGLQNIAVVRTAVGAASGTIDIVDNSGGILGDRVP 100
Cdd:TIGR01444   1 VVIDVGANIGDTSLYFA-RKGAEGRVIAFEPLPDAYEILEENVKLNNLPNVVLLNAAVGDRDGELEFNVSDDDTGNSSLL 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1125431534 101 GMRVL------QVAMTSLDEFFGER---LPNLLKIDVEGYEFDVLTGARRCL-AARPKIALEFH 154
Cdd:TIGR01444  80 PTPDAdreseiEVEVVTLDDLVEEFgldKVDLLKIDVEGAELEVLRGAAETLlEKRPVIVLEVH 143
Methyltransf_21 pfam05050
Methyltransferase FkbM domain; This family has members from bacteria to human, and appears to ...
 24-179 1.64e-20

Methyltransferase FkbM domain; This family has members from bacteria to human, and appears to be a methyltransferase.


Pssm-ID: 428282  Cd Length: 170  Bit Score: 84.54  E-value: 1.64e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  24 DVGAHHG---YFSVLMAHWVGARGKVYAFECLPENA---DIASRNVALN---GLQNIAVVRTAVGAASGTIDIvDNSGGI 94
Cdd:pfam05050   1 DVGANDGvwdSVALLFEKKCGGGGEVLAIEPNPNKLeklDCTLLNLALGndvGLYEFYLGGKGGGGYLLFAVG-DPQGAS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  95 LGDRVPGMRV--LQVAMTSLDEFFGERLP---NLLKIDVEGYEFDVLTGARRCLAA-RPK-IALEFHCFKFADPVEHvAR 167
Cdd:pfam05050  80 TSSVLGGEEAkyIEVETVTLDSFLEEIKKsdiDLLKIDVEGAELEVLEGAEKTLKRcQPNiIVIEVHFFHYFGGPLF-DE 158

                         170
                  ....*....|..
gi 1125431534 168 VLDLLPRAGYEY 179
Cdd:pfam05050 159 IRQFLRECGYRL 170
COG4076 COG4076
Predicted RNA methylase [General function prediction only];
12-74 4.29e-08

Predicted RNA methylase [General function prediction only];


Pssm-ID: 443253 [Multi-domain]  Cd Length: 230  Bit Score: 51.96  E-value: 4.29e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1125431534  12 LARAAEPGDVVADVGAHHGYFSVLMAHwVGARgKVYAFECLPENADIASRNVALNGL-QNIAVV 74
Cdd:COG4076    29 IERVVKPGDVVLDIGTGSGLLSMLAAR-AGAK-KVYAVEVNPDIAAVARRIIAANGLsDRITVI 90
PRK13942 PRK13942
protein-L-isoaspartate O-methyltransferase; Provisional
 18-74 5.29e-06

protein-L-isoaspartate O-methyltransferase; Provisional


Pssm-ID: 184409  Cd Length: 212  Bit Score: 45.78  E-value: 5.29e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1125431534  18 PGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGLQNIAVV 74
Cdd:PRK13942   76 EGMKVLEIGTGSGYHAAVVAEIVGKSGKVVTIERIPELAEKAKKTLKKLGYDNVEVI 132
 
Name Accession Description Interval E-value
fkbM_fam TIGR01444
methyltransferase, FkbM family; Members of this family are characterized by two well-conserved ...
 21-154 3.24e-31

methyltransferase, FkbM family; Members of this family are characterized by two well-conserved short regions separated by a variable in both sequence and length. The first of the two regions is found in a large number of proteins outside this subfamily, a number of which have been characterized as methyltransferases. One member of the present family, FkbM, was shown to be required for a specific methylation in the biosynthesis of the immunosuppressant FK506 in Streptomyces strain MA6548.


Pssm-ID: 273628  Cd Length: 143  Bit Score: 111.63  E-value: 3.24e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  21 VVADVGAHHGYFSVLMAhWVGARGKVYAFECLPENADIASRNVALNGLQNIAVVRTAVGAASGTIDIVDNSGGILGDRVP 100
Cdd:TIGR01444   1 VVIDVGANIGDTSLYFA-RKGAEGRVIAFEPLPDAYEILEENVKLNNLPNVVLLNAAVGDRDGELEFNVSDDDTGNSSLL 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1125431534 101 GMRVL------QVAMTSLDEFFGER---LPNLLKIDVEGYEFDVLTGARRCL-AARPKIALEFH 154
Cdd:TIGR01444  80 PTPDAdreseiEVEVVTLDDLVEEFgldKVDLLKIDVEGAELEVLRGAAETLlEKRPVIVLEVH 143
Methyltransf_21 pfam05050
Methyltransferase FkbM domain; This family has members from bacteria to human, and appears to ...
 24-179 1.64e-20

Methyltransferase FkbM domain; This family has members from bacteria to human, and appears to be a methyltransferase.


Pssm-ID: 428282  Cd Length: 170  Bit Score: 84.54  E-value: 1.64e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  24 DVGAHHG---YFSVLMAHWVGARGKVYAFECLPENA---DIASRNVALN---GLQNIAVVRTAVGAASGTIDIvDNSGGI 94
Cdd:pfam05050   1 DVGANDGvwdSVALLFEKKCGGGGEVLAIEPNPNKLeklDCTLLNLALGndvGLYEFYLGGKGGGGYLLFAVG-DPQGAS 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  95 LGDRVPGMRV--LQVAMTSLDEFFGERLP---NLLKIDVEGYEFDVLTGARRCLAA-RPK-IALEFHCFKFADPVEHvAR 167
Cdd:pfam05050  80 TSSVLGGEEAkyIEVETVTLDSFLEEIKKsdiDLLKIDVEGAELEVLEGAEKTLKRcQPNiIVIEVHFFHYFGGPLF-DE 158

                         170
                  ....*....|..
gi 1125431534 168 VLDLLPRAGYEY 179
Cdd:pfam05050 159 IRQFLRECGYRL 170
COG4076 COG4076
Predicted RNA methylase [General function prediction only];
12-74 4.29e-08

Predicted RNA methylase [General function prediction only];


Pssm-ID: 443253 [Multi-domain]  Cd Length: 230  Bit Score: 51.96  E-value: 4.29e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1125431534  12 LARAAEPGDVVADVGAHHGYFSVLMAHwVGARgKVYAFECLPENADIASRNVALNGL-QNIAVV 74
Cdd:COG4076    29 IERVVKPGDVVLDIGTGSGLLSMLAAR-AGAK-KVYAVEVNPDIAAVARRIIAANGLsDRITVI 90
PRK13942 PRK13942
protein-L-isoaspartate O-methyltransferase; Provisional
 18-74 5.29e-06

protein-L-isoaspartate O-methyltransferase; Provisional


Pssm-ID: 184409  Cd Length: 212  Bit Score: 45.78  E-value: 5.29e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1125431534  18 PGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGLQNIAVV 74
Cdd:PRK13942   76 EGMKVLEIGTGSGYHAAVVAEIVGKSGKVVTIERIPELAEKAKKTLKKLGYDNVEVI 132
PCMT pfam01135
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT);
18-74 5.60e-06

Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT);


Pssm-ID: 395902 [Multi-domain]  Cd Length: 205  Bit Score: 45.44  E-value: 5.60e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1125431534  18 PGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGLQNIAVV 74
Cdd:pfam01135  73 PGMRVLEIGSGSGYLTACFARMVGEVGRVVSIEHIPELVEIARRNLEKLGLENVIVV 129
Gcd14 COG2519
tRNA A58 N-methylase Trm61 [Translation, ribosomal structure and biogenesis]; tRNA A58 ...
 18-68 2.15e-05

tRNA A58 N-methylase Trm61 [Translation, ribosomal structure and biogenesis]; tRNA A58 N-methylase Trm61 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 442009 [Multi-domain]  Cd Length: 249  Bit Score: 43.99  E-value: 2.15e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1125431534  18 PGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGL 68
Cdd:COG2519    91 PGARVLEAGTGSGALTLALARAVGPEGKVYSYERREDFAEIARKNLERFGL 141
Met_10 pfam02475
Met-10+ like-protein; The methionine-10 mutant allele of N. crassa codes for a protein of ...
 1-71 2.53e-05

Met-10+ like-protein; The methionine-10 mutant allele of N. crassa codes for a protein of unknown function, Swiss:O27901. However, homologous proteins have been found in yeast suggesting this protein may be involved in methionine biosynthesis, transport and/or utilization.


Pssm-ID: 396850 [Multi-domain]  Cd Length: 198  Bit Score: 43.49  E-value: 2.53e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1125431534   1 MYTP-LRAELAWLARAAEPGDVVADVGAHHGYFSVLMAHWVGARgKVYAFECLPENADIASRNVALNGLQNI 71
Cdd:pfam02475  81 YWSPrLIAERERIAKLVEPGEVVVDMFAGIGPFSIPIAKHSKAR-RVYAIELNPESYKYLKENIKLNKVEDV 151
TrmN6 COG4123
tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) ...
 9-77 1.89e-03

tRNA1(Val) A37 N6-methylase TrmN6 [Translation, ribosomal structure and biogenesis]; tRNA1(Val) A37 N6-methylase TrmN6 is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 443299 [Multi-domain]  Cd Length: 238  Bit Score: 38.20  E-value: 1.89e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1125431534   9 LAWLARAaEPGDVVADVGAHHGYFSVLMAHWVGArGKVYAFECLPENADIASRNVALNGLQNIAVVRTA 77
Cdd:COG4123    29 LAAFAPV-KKGGRVLDLGTGTGVIALMLAQRSPG-ARITGVEIQPEAAELARRNVALNGLEDRITVIHG 95
cbiT PRK00377
cobalt-precorrin-6Y C(15)-methyltransferase; Provisional
 19-75 2.23e-03

cobalt-precorrin-6Y C(15)-methyltransferase; Provisional


Pssm-ID: 234740  Cd Length: 198  Bit Score: 37.85  E-value: 2.23e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1125431534  19 GDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNG-LQNIAVVR 75
Cdd:PRK00377   41 GDMILDIGCGTGSVTVEASLLVGETGKVYAVDKDEKAINLTRRNAEKFGvLNNIVLIK 98
CobL COG2242
Precorrin-6B methylase 2 [Coenzyme transport and metabolism]; Precorrin-6B methylase 2 is part ...
 12-75 2.70e-03

Precorrin-6B methylase 2 [Coenzyme transport and metabolism]; Precorrin-6B methylase 2 is part of the Pathway/BioSystem: Cobalamine/B12 biosynthesis


Pssm-ID: 441843 [Multi-domain]  Cd Length: 403  Bit Score: 38.22  E-value: 2.70e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1125431534  12 LARAA-EPGDVVADVGAHHGYFSVLMAHWvGARGKVYAFECLPENADIASRNVALNGLQNIAVVR 75
Cdd:COG2242   240 LAKLAlRPGDVLWDIGAGSGSVSIEAARL-APGGRVYAIERDPERAALIRANARRFGVPNVEVVE 303
arsM PRK11873
arsenite methyltransferase;
18-71 3.98e-03

arsenite methyltransferase;


Pssm-ID: 237007 [Multi-domain]  Cd Length: 272  Bit Score: 37.24  E-value: 3.98e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1125431534  18 PGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGLQNI 71
Cdd:PRK11873   77 PGETVLDLGSGGGFDCFLAARRVGPTGKVIGVDMTPEMLAKARANARKAGYTNV 130
COG4798 COG4798
Predicted methyltransferase [General function prediction only];
17-88 4.39e-03

Predicted methyltransferase [General function prediction only];


Pssm-ID: 443826  Cd Length: 274  Bit Score: 37.20  E-value: 4.39e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534  17 EPGDVVADVGAHHGYFSVLMAHWVGARGKVYAFECLPENADIASRNVALNGLQ-----------NIAVVR----TAVGAA 81
Cdd:COG4798    65 KPGMTVVEIWPGGGWYTEILAPYLGPKGKVYAANFDPDSEPPEYAKRSREAFSaklaadpalygNVRVTAfappDDPIAP 144


                  ....*..
gi 1125431534  82 SGTIDIV 88
Cdd:COG4798   145 PGSADLV 151
PrmA COG2264
Ribosomal protein L11 methylase PrmA [Translation, ribosomal structure and biogenesis];
9-88 9.50e-03

Ribosomal protein L11 methylase PrmA [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441865 [Multi-domain]  Cd Length: 284  Bit Score: 36.30  E-value: 9.50e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125431534   9 LAWLARAAEPGDVVADVGAHHGYFSVLMAHWvGARgKVYAFeclpenaDI------ASR-NVALNGLQNIAVVRTAVGAA 81
Cdd:COG2264   139 LEALEKLLKPGKTVLDVGCGSGILAIAAAKL-GAK-RVLAV-------DIdpvaveAAReNAELNGVEDRIEVVLGDLLE 209


                  ....*..
gi 1125431534  82 SGTIDIV 88
Cdd:COG2264   210 DGPYDLV 216
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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