Translin and translin-associated factor-X (TRAX); Translin (also known as TB-RBP), and its binding partner protein TRAX (translin-associated factor-X) are a paralogous pair of conserved proteins, and oligomeric complexes of TRAX and translin are known as C3PO proteins (for component 3 promoter of RNA-induced silencing complex or RISC). The Translin-Trax complex enhances the removal of the passenger strand in RNAi and the formation of active RISC. Translin and Trax participate in a variety of nucleic acid metabolism pathways in addition to RNAi and have been implicated in a wide range of biological activities, including mRNA processing, cell growth regulation, spermatogenesis, neuronal development/function, genome stability regulation and carcinogenesis; however, their precise role in some of the processes remains unclear. It has been shown that Trax subunit, but not Translin, possesses a Glu-Glu-Asp catalytic center with the capacity to digest RNA as well as DNA; this catalytic activity is required for passenger-strand removal and RISC activation in RNAi. In Archaeoglobus fulgidus, Trax-like-subunits assemble into an octameric structure, highly similar to human C3PO; its complex with duplex RNA reveals that the octamer entirely encapsulates a single 13-base-pair RNA duplex inside a large inner cavity.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226  37 YSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDSRKQNKEKVLEEARQRLNKLIAvNFRAVALELRDQDVYQFRSS 116
Cdd:cd14805    1 YQGELDAEQDIRERIRKVVRDIEQESKEIIFLLQGIHSGKGDIEKRCLEAREHLNTVKQ-KLTSLATELPAEQYYRFHEH 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 117 YSPGLQEFIEAYTYMEYLCHEDAEGENETKSVSdwqaiqavmqyveessqpkeeptegedvqaiaQVESPKKFQFFVDPT 196
Cdd:cd14805   80 WTFGLQRLVFAAAFVVYLETETLVTREEVTEIL--------------------------------GIEPDREKGFHLDVE 127

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939620226 197 EYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRaRELWRKITTMKQSVLKAENV 267
Cdd:cd14805  128 DYLLGVLDLASELSRLCINSVTAGDYDRPLHISTFINELYSGFRLLNLKN-DELRKRYDTLKYDVKKVEEV 197

Translin and translin-associated factor-X (TRAX); Translin (also known as TB-RBP), and its binding partner protein TRAX (translin-associated factor-X) are a paralogous pair of conserved proteins, and oligomeric complexes of TRAX and translin are known as C3PO proteins (for component 3 promoter of RNA-induced silencing complex or RISC). The Translin-Trax complex enhances the removal of the passenger strand in RNAi and the formation of active RISC. Translin and Trax participate in a variety of nucleic acid metabolism pathways in addition to RNAi and have been implicated in a wide range of biological activities, including mRNA processing, cell growth regulation, spermatogenesis, neuronal development/function, genome stability regulation and carcinogenesis; however, their precise role in some of the processes remains unclear. It has been shown that Trax subunit, but not Translin, possesses a Glu-Glu-Asp catalytic center with the capacity to digest RNA as well as DNA; this catalytic activity is required for passenger-strand removal and RISC activation in RNAi. In Archaeoglobus fulgidus, Trax-like-subunits assemble into an octameric structure, highly similar to human C3PO; its complex with duplex RNA reveals that the octamer entirely encapsulates a single 13-base-pair RNA duplex inside a large inner cavity.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226  37 YSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDSRKQNKEKVLEEARQRLNKLIAvNFRAVALELRDQDVYQFRSS 116
Cdd:cd14805    1 YQGELDAEQDIRERIRKVVRDIEQESKEIIFLLQGIHSGKGDIEKRCLEAREHLNTVKQ-KLTSLATELPAEQYYRFHEH 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 117 YSPGLQEFIEAYTYMEYLCHEDAEGENETKSVSdwqaiqavmqyveessqpkeeptegedvqaiaQVESPKKFQFFVDPT 196
Cdd:cd14805   80 WTFGLQRLVFAAAFVVYLETETLVTREEVTEIL--------------------------------GIEPDREKGFHLDVE 127

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939620226 197 EYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRaRELWRKITTMKQSVLKAENV 267
Cdd:cd14805  128 DYLLGVLDLASELSRLCINSVTAGDYDRPLHISTFINELYSGFRLLNLKN-DELRKRYDTLKYDVKKVEEV 197

haloacid dehalogenase superfamily protein; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226  44 KHDRHERIVKLSRDITIESKRIIFLLHsidsRKQnkekvLEEARQRLNKLiavnfRAVALELRDQ-----DVYQFRSSYS 118
Cdd:PRK14562  16 KDEAREEALKLSREIVRLSGDAIRAIH----RGD-----FEEAEKLLKEA-----EELVKELKELlkdhpELYYAGYVGT 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 119 PgLQEFIEAYTYMEYLchedaegenetksvsdwqaiqavmqyveessqpKEEPTegedvqaiaqvesPKKFQFFVDPTEY 198
Cdd:PRK14562  82 A-LQEYVEALLVYSLL---------------------------------FENKI-------------PSPEELGVPEAAY 114

                        170       180
                 ....*....|....*....|....*
gi 939620226 199 ILGLSDLTGELMRRCINSLGSGDTD 223
Cdd:PRK14562 115 LLGLADAIGELRRHILELLRKGEIE 139

Translin and translin-associated factor-X (TRAX); Translin (also known as TB-RBP), and its binding partner protein TRAX (translin-associated factor-X) are a paralogous pair of conserved proteins, and oligomeric complexes of TRAX and translin are known as C3PO proteins (for component 3 promoter of RNA-induced silencing complex or RISC). The Translin-Trax complex enhances the removal of the passenger strand in RNAi and the formation of active RISC. Translin and Trax participate in a variety of nucleic acid metabolism pathways in addition to RNAi and have been implicated in a wide range of biological activities, including mRNA processing, cell growth regulation, spermatogenesis, neuronal development/function, genome stability regulation and carcinogenesis; however, their precise role in some of the processes remains unclear. It has been shown that Trax subunit, but not Translin, possesses a Glu-Glu-Asp catalytic center with the capacity to digest RNA as well as DNA; this catalytic activity is required for passenger-strand removal and RISC activation in RNAi. In Archaeoglobus fulgidus, Trax-like-subunits assemble into an octameric structure, highly similar to human C3PO; its complex with duplex RNA reveals that the octamer entirely encapsulates a single 13-base-pair RNA duplex inside a large inner cavity.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226  37 YSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDSRKQNKEKVLEEARQRLNKLIAvNFRAVALELRDQDVYQFRSS 116
Cdd:cd14805    1 YQGELDAEQDIRERIRKVVRDIEQESKEIIFLLQGIHSGKGDIEKRCLEAREHLNTVKQ-KLTSLATELPAEQYYRFHEH 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 117 YSPGLQEFIEAYTYMEYLCHEDAEGENETKSVSdwqaiqavmqyveessqpkeeptegedvqaiaQVESPKKFQFFVDPT 196
Cdd:cd14805   80 WTFGLQRLVFAAAFVVYLETETLVTREEVTEIL--------------------------------GIEPDREKGFHLDVE 127

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 939620226 197 EYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRaRELWRKITTMKQSVLKAENV 267
Cdd:cd14805  128 DYLLGVLDLASELSRLCINSVTAGDYDRPLHISTFINELYSGFRLLNLKN-DELRKRYDTLKYDVKKVEEV 197

Translin-associated factor-X (TRAX); TRAX (translin-associated factor-X) is a paralog of its binding partner protein Translin and together they form oligomeric complexes known as C3PO proteins (for component 3 promoter of RNA-induced silencing complex or RISC). TRAX complexed with Translin is possibly involved in dendritic RNA processing and in DNA double-strand break repair as an interacting partner with C1D, an activator of the DNA-dependent protein kinase involved in the repair of DNA-double strand breaks. It has been shown that Trax subunit, but not Translin, possesses a Glu-Glu-Asp catalytic center with the capacity to digest RNA; this catalytic activity is required for passenger-strand removal and RISC activation in RNAi. In Archaeoglobus fulgidus, Trax-like-subunits assemble into an octameric structure, highly similar to human C3PO; its complex with duplex RNA reveals that the octamer entirely encapsulates a single 13-base-pair RNA duplex inside a large inner cavity. Translin and Trax participate in a variety of nucleic acid metabolism pathways in addition to RNAi and have been implicated in a wide range of biological activities, including mRNA processing, cell growth regulation, spermatogenesis, neuronal development/function, genome stability regulation and carcinogenesis; however, their precise role in some of the processes remains unclear.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226  34 FRIYSNELIMKHDRHERIVKLSRDITIESKRIIFLLHSIDsrKQNKEKVLEEARQRLNKLIAvnfravalELRDQDVYQF 113
Cdd:cd14820    1 FESIRKELDEKDDKRERLIKLSRDITRLSKRAIFALHRGD--LEEAEELLKEARELLEELRE--------LLKEEPDLLY 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 114 RSSYSPGLQEFIEAYTYMEYLCHEDAEGENEtksvsdwqaiqavmqyveessqpkeeptegedvqaiaqvespkkfQFFV 193
Cdd:cd14820   71 SGAVSPALQEYVEALSFYHFLKEGRLPTPDE---------------------------------------------ELGV 105

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 939620226 194 DPTEYILGLSDLTGELMRRCINSLGSGDTDTCLDTCKALQHFYSGYISLNCQRARELWRKITTMKQSVLKAENVCYN 270
Cdd:cd14820  106 TPEDYLLGLADLTGELMRYAIDSLRKGDLEEAEKYLEFMEEIYEGLMSFDYPDALELRKKLDVDRQSLLKEETRCYE 182

Translin, also known as TB-RBP (testis brain RNA-binding protein); Translin (also known as TB-RBP for Testis Brain RNA-binding protein, a mouse ortholog), is a paralog of its binding partner protein TRAX (translin-associated factor-X) and together they form oligomeric complexes known as C3PO proteins (for component 3 promoter of RNA-induced silencing complex or RISC). DNA damage has been proposed to stimulate transport of Translin into nuclei. It binds to RNA and single-stranded DNA, and its selectivity is modulated by interactions with GTP and TRAX. Translin may also regulate dendritic trafficking of BDNF RNAs as well as function as a key activator of siRNA-mediated silencing in drosophila. Translin and Trax participate in a variety of nucleic acid metabolism pathways in addition to RNAi and have been implicated in a wide range of biological activities, including mRNA processing, cell growth regulation, spermatogenesis, neuronal development/function, genome stability regulation and carcinogenesis; however, their precise role in some of the processes remains unclear.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 939620226 175 EDVQAIAQVESPKKFQFFVDPTEYILGLSDLTGELMRRCINSLGSGDTDTCL---DTCKALqhfYSGYISLNCQ----Ra 247
Cdd:cd14819  107 EEVAEILGVPVNLKDGFHLDLEDYLHGLLSLVNELSRLAVNSVTLGDYERPLkisAFVKDL---HAGFQLLNLKndslR- 182

                         90       100
                 ....*....|....*....|....*...
gi 939620226 248 relwRKITTMKQSVLKAENVCYNVKVRG 275
Cdd:cd14819  183 ----KRFDGLKYDVKKVEEVVYDLSLRG 206