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Conserved domains on  [gi|24652848|ref|NP_610713|]
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rebuf [Drosophila melanogaster]

Protein Classification

mothers against decapentaplegic homolog( domain architecture ID 10649998)

mothers against decapentaplegic homolog such as SMAD1, SMAD5 and SMAD9 (also known as SMAD8); all closely related receptor regulated SMADs (R-SMADs). SMAD1 plays an essential role in bone development and postnatal bone formation through activation by bone morphogenetic protein (BMP) type 1 receptor kinase. SMAD5 is involved in bone morphogenetic proteins (BMP) signal modulation and may also play a role in the pathway involving inhibition of hematopoietic progenitor cells by TGF-beta. SMAD9 mediates the differentiation of mesenchymal stem cells (MSCs) into tendon-like cells by inhibiting the osteogenic pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DWB smart00524
Domain B in dwarfin family proteins;
53-220 1.92e-59

Domain B in dwarfin family proteins;


:

Pssm-ID: 197770  Cd Length: 171  Bit Score: 191.75  E-value: 1.92e-59
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848     53 IWAKIIVFEKNRRVAKAYARSS-VITINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMDDtGNIFIKRYG 131
Cdd:smart00524   1 SWCKIAYYELNTRVGETFKVSSpSVTVDGFTDPSDGNRFCLGQLSNVNRNEATELIRKHIGKGVQLSYEN-GDVWLYNRS 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848    132 KSSIYVNSTSQGNeetvIGGDIIqMPQMSLTAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK------ 205
Cdd:smart00524  80 DSPIFVQSPYLDE----PGGRTL-DTVHKLPPGYSIKVFDMEKFAQLLARELAKGFEGVYDLARMCTIRISFVKgwgpdy 154

                          170
                   ....*....|....*
gi 24652848    206 SNNNLINSPVWILVV 220
Cdd:smart00524 155 SRQTITSTPCWIEVH 169
 
Name Accession Description Interval E-value
DWB smart00524
Domain B in dwarfin family proteins;
53-220 1.92e-59

Domain B in dwarfin family proteins;


Pssm-ID: 197770  Cd Length: 171  Bit Score: 191.75  E-value: 1.92e-59
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848     53 IWAKIIVFEKNRRVAKAYARSS-VITINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMDDtGNIFIKRYG 131
Cdd:smart00524   1 SWCKIAYYELNTRVGETFKVSSpSVTVDGFTDPSDGNRFCLGQLSNVNRNEATELIRKHIGKGVQLSYEN-GDVWLYNRS 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848    132 KSSIYVNSTSQGNeetvIGGDIIqMPQMSLTAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK------ 205
Cdd:smart00524  80 DSPIFVQSPYLDE----PGGRTL-DTVHKLPPGYSIKVFDMEKFAQLLARELAKGFEGVYDLARMCTIRISFVKgwgpdy 154

                          170
                   ....*....|....*
gi 24652848    206 SNNNLINSPVWILVV 220
Cdd:smart00524 155 SRQTITSTPCWIEVH 169
MH2 pfam03166
MH2 domain; This is the MH2 (MAD homology 2) domain found at the carboxy terminus of MAD ...
 52-222 9.39e-48

MH2 domain; This is the MH2 (MAD homology 2) domain found at the carboxy terminus of MAD related proteins such as Smads. This domain is separated from the MH1 domain by a non-conserved linker region. The MH2 domain mediates interaction with a wide variety of proteins and provides specificity and selectivity to Smad function and also is critical for mediating interactions in Smad oligomers. Unlike MH1, MH2 does not bind DNA. The well-studied MH2 domain of Smad4 is composed of five alpha helices and three loops enclosing a beta sandwich. Smads are involved in the propagation of TGF-beta signals by direct association with the TGF-beta receptor kinase which phosphorylates the last two Ser of a conserved 'SSXS' motif located at the C-terminus of MH2.


Pssm-ID: 460834  Cd Length: 172  Bit Score: 161.25  E-value: 9.39e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848    52 EIWAKIIVFEKNRRVAKAYARSS-VITINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMDDtGNIFIKRY 130
Cdd:pfam03166   1 EIWCSVAYYELNTRVGEAFKVSSpNVTVDGFTDPSDGNRFCLGLLSNVNRNEAVEKVRKHIGKGVRLSYDG-GEVWIYNL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848   131 GKSSIYVNSTSQGNEETVIGGDIIQ-MPQMSLtaatsaRLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK---- 205
Cdd:pfam03166  80 SDHPVFVQSPYLNREAGRAPDTVHKvPPGESL------KVFDMRKFQQLLSQELRRARLGPQDANKLCSVRISFVKgwgp 153

                         170
                  ....*....|....*....
gi 24652848   206 --SNNNLINSPVWILVVNV 222
Cdd:pfam03166 154 dySRQDITSTPCWIEIHLH 172
MH2 cd00050
C-terminal Mad Homology 2 (MH2) domain; The MH2 domain is found in the SMAD (small mothers ...
 54-217 5.92e-05

C-terminal Mad Homology 2 (MH2) domain; The MH2 domain is found in the SMAD (small mothers against decapentaplegic) family of proteins and is responsible for type I receptor interactions, phosphorylation-triggered homo- and hetero-oligomerization, and transactivation. It is negatively regulated by the N-terminal MH1 domain which prevents it from forming a complex with SMAD4. The MH2 domain is multifunctional and provides SMADs with their specificity and selectivity, as well as transcriptional activity. Several transcriptional co-activators and repressors have also been reported to regulate SMAD signaling by interacting with the MH2 domain. Mutations in the MH2 domains of SMAD2 and especially SMAD4 have been detected in colorectal and other human cancers.


Pssm-ID: 199819  Cd Length: 170  Bit Score: 43.36  E-value: 5.92e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848  54 WAKIIVFEKNRRVAKAYARSSVI-TINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMdDTGNIFIKRYGK 132
Cdd:cd00050   1 WCSIAYYELNTRVGELFHVYSPSvAVDGFTDPSNGDRFCLGQLSNVNRNETIERTRRHIGKGVHLYY-VGGEVWAECLSD 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848 133 SSIYVNSTSQGNEETVIGGDIIQMPqmsltAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK------S 206
Cdd:cd00050  80 HAIFVQSRNLDYPHGRHPLTVCKIP-----PGCSIKVFDNQEFAQLLHQSVNTGFEGVYELTKMCTIRMSFVKgwgpeyH 154

                       170
                ....*....|.
gi 24652848 207 NNNLINSPVWI 217
Cdd:cd00050 155 RQDITSTPCWI 165
 
Name Accession Description Interval E-value
DWB smart00524
Domain B in dwarfin family proteins;
53-220 1.92e-59

Domain B in dwarfin family proteins;


Pssm-ID: 197770  Cd Length: 171  Bit Score: 191.75  E-value: 1.92e-59
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848     53 IWAKIIVFEKNRRVAKAYARSS-VITINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMDDtGNIFIKRYG 131
Cdd:smart00524   1 SWCKIAYYELNTRVGETFKVSSpSVTVDGFTDPSDGNRFCLGQLSNVNRNEATELIRKHIGKGVQLSYEN-GDVWLYNRS 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848    132 KSSIYVNSTSQGNeetvIGGDIIqMPQMSLTAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK------ 205
Cdd:smart00524  80 DSPIFVQSPYLDE----PGGRTL-DTVHKLPPGYSIKVFDMEKFAQLLARELAKGFEGVYDLARMCTIRISFVKgwgpdy 154

                          170
                   ....*....|....*
gi 24652848    206 SNNNLINSPVWILVV 220
Cdd:smart00524 155 SRQTITSTPCWIEVH 169
MH2 pfam03166
MH2 domain; This is the MH2 (MAD homology 2) domain found at the carboxy terminus of MAD ...
 52-222 9.39e-48

MH2 domain; This is the MH2 (MAD homology 2) domain found at the carboxy terminus of MAD related proteins such as Smads. This domain is separated from the MH1 domain by a non-conserved linker region. The MH2 domain mediates interaction with a wide variety of proteins and provides specificity and selectivity to Smad function and also is critical for mediating interactions in Smad oligomers. Unlike MH1, MH2 does not bind DNA. The well-studied MH2 domain of Smad4 is composed of five alpha helices and three loops enclosing a beta sandwich. Smads are involved in the propagation of TGF-beta signals by direct association with the TGF-beta receptor kinase which phosphorylates the last two Ser of a conserved 'SSXS' motif located at the C-terminus of MH2.


Pssm-ID: 460834  Cd Length: 172  Bit Score: 161.25  E-value: 9.39e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848    52 EIWAKIIVFEKNRRVAKAYARSS-VITINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMDDtGNIFIKRY 130
Cdd:pfam03166   1 EIWCSVAYYELNTRVGEAFKVSSpNVTVDGFTDPSDGNRFCLGLLSNVNRNEAVEKVRKHIGKGVRLSYDG-GEVWIYNL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848   131 GKSSIYVNSTSQGNEETVIGGDIIQ-MPQMSLtaatsaRLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK---- 205
Cdd:pfam03166  80 SDHPVFVQSPYLNREAGRAPDTVHKvPPGESL------KVFDMRKFQQLLSQELRRARLGPQDANKLCSVRISFVKgwgp 153

                         170
                  ....*....|....*....
gi 24652848   206 --SNNNLINSPVWILVVNV 222
Cdd:pfam03166 154 dySRQDITSTPCWIEIHLH 172
MH2 cd00050
C-terminal Mad Homology 2 (MH2) domain; The MH2 domain is found in the SMAD (small mothers ...
 54-217 5.92e-05

C-terminal Mad Homology 2 (MH2) domain; The MH2 domain is found in the SMAD (small mothers against decapentaplegic) family of proteins and is responsible for type I receptor interactions, phosphorylation-triggered homo- and hetero-oligomerization, and transactivation. It is negatively regulated by the N-terminal MH1 domain which prevents it from forming a complex with SMAD4. The MH2 domain is multifunctional and provides SMADs with their specificity and selectivity, as well as transcriptional activity. Several transcriptional co-activators and repressors have also been reported to regulate SMAD signaling by interacting with the MH2 domain. Mutations in the MH2 domains of SMAD2 and especially SMAD4 have been detected in colorectal and other human cancers.


Pssm-ID: 199819  Cd Length: 170  Bit Score: 43.36  E-value: 5.92e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848  54 WAKIIVFEKNRRVAKAYARSSVI-TINGSKNGFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMdDTGNIFIKRYGK 132
Cdd:cd00050   1 WCSIAYYELNTRVGELFHVYSPSvAVDGFTDPSNGDRFCLGQLSNVNRNETIERTRRHIGKGVHLYY-VGGEVWAECLSD 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848 133 SSIYVNSTSQGNEETVIGGDIIQMPqmsltAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVK------S 206
Cdd:cd00050  80 HAIFVQSRNLDYPHGRHPLTVCKIP-----PGCSIKVFDNQEFAQLLHQSVNTGFEGVYELTKMCTIRMSFVKgwgpeyH 154

                       170
                ....*....|.
gi 24652848 207 NNNLINSPVWI 217
Cdd:cd00050 155 RQDITSTPCWI 165
MH2_SMAD_1_5_9 cd10497
C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9; The MH2 domain is located at ...
 54-259 7.27e-03

C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9; The MH2 domain is located at the C-terminus of the SMAD (small mothers against decapentaplegic) family of proteins, which are signal transducers and transcriptional modulators that mediate multiple signaling pathways. The MH2 domain is responsible for type I receptor interaction, phosphorylation-triggered homo- and hetero-oligomerization, and transactivation. It is negatively regulated by the N-terminal MH1 domain, which prevents it from forming a complex with SMAD4. SMAD1, SMAD5 and SMAD9 (also known as SMAD8), are receptor regulated SMADs (R-SMADs). SMAD1 plays an essential role in bone development and postnatal bone formation through activation by bone morphogenetic protein (BMP) type 1 receptor kinase. SMAD5 is involved in BMP signal modulation and may also play a role in the pathway involving inhibition of hematopoietic progenitor cells by TGF-beta. SMAD9 mediates the differentiation of mesenchymal stem cells (MSCs) into tendon-like cells by inhibiting the osteogenic pathway.


Pssm-ID: 199822  Cd Length: 201  Bit Score: 37.55  E-value: 7.27e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848  54 WAKIIVFEKNRRVAKAY-ARSSVITINGSKN-GFDGVRIGLNGFDNPMRDAETKVIKKSIGDGFKIKMdDTGNIFIKRYG 131
Cdd:cd10497   7 WCSIAYYELNNRVGEAFhASSTSIIVDGFTDpSNNSDRFCLGLLSNVNRNSTIENTRRHIGKGVHLYY-VGGEVYAECLS 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24652848 132 KSSIYVNSTSQGNEETVIGGDIIQMPqmsltAATSARLFDMKKFQTNINREFARTYPERGRLERQCLSAVSLVKS----- 206
Cdd:cd10497  86 DSSIFVQSRNCNYHHGFHPTTVCKIP-----PGCSLKIFNNQEFAQLLSQSVNHGFEAVYELTKMCTIRMSFVKGwgaey 160

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 24652848 207 -NNNLINSPVWILVvnvvamdMLRSRLQTIPKSLDAMGmrvpltnSSEDPYSTI 259
Cdd:cd10497 161 hRQDVTSTPCWIEI-------HLHGPLQWLDKVLTQMG-------SPHNPISSV 200
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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