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Conserved domains on  [gi|6325290|ref|NP_015358|]
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histidine--tRNA ligase [Saccharomyces cerevisiae S288C]

Protein Classification

histidine--tRNA ligase( domain architecture ID 1007767)

histidine--tRNA ligase (HisRS) is responsible for the attachment of histidine to the 3' OH group of ribose of the appropriate tRNA

CATH:  3.40.50.800|3.30.930.10
EC:  6.1.1.21
Gene Ontology:  GO:0003723|GO:0042802|GO:0004821|GO:0006427|GO:0005524|GO:0006412

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PLN02972 super family cl33611
Histidyl-tRNA synthetase
 56-503 6.33e-165

Histidyl-tRNA synthetase


The actual alignment was detected with superfamily member PLN02972:

Pssm-ID: 215525 [Multi-domain]  Cd Length: 763  Bit Score: 487.09  E-value: 6.33e-165
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    56 KTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFA 135
Cdd:PLN02972 328 KIPKGTRDFAKEQMAIREKAFSIITSVFKRHGATALDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFA 407

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   136 RYVAMNNIQSIKRYHIAKVYRRDQPamTKGRMREFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKI 215
Cdd:PLN02972 408 RYVAMNGITSFKRYQIAKVYRRDNP--SKGRYREFYQCDFDIAGVYEPMGPDFEIIKVLTELLDELDIGTYEVKLNHRKL 485

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   216 LDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSA-DANITSNEK 294
Cdd:PLN02972 486 LDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSNETADKIGNFVKERGPPLELLSKLRQeGSEFLGNAS 565

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   295 AKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaselkkkAKSAEdasefvgVGSIAAG 374
Cdd:PLN02972 566 SRAALDELEIMFKALEKSKAIGKIVFDLSLARGLDYYTGVIYEAV----------------FKGAQ-------VGSIAAG 622

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   375 GRYDNLVNMFseaSGKkstQIPCVGISFGVERIFSLI-KQRINSSTTIKPTATQVFVMAFGGGKdwtgyLPERMKVTKQL 453
Cdd:PLN02972 623 GRYDNLVGMF---SGK---QVPAVGVSLGIERVFAIMeQQEEEKSQVIRPTETEVLVSIIGDDK-----LALAAELVSEL 691

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|..
gi 6325290   454 WDAGIEAEYvykaKANPRKQ--FDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:PLN02972 692 WNAGIKAEY----KVSTRKAkhLKRAKESGIPWMVLVGEKELSKGFVKLKNL 739
 
Name Accession Description Interval E-value
PLN02972 PLN02972
Histidyl-tRNA synthetase
 56-503 6.33e-165

Histidyl-tRNA synthetase


Pssm-ID: 215525 [Multi-domain]  Cd Length: 763  Bit Score: 487.09  E-value: 6.33e-165
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    56 KTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFA 135
Cdd:PLN02972 328 KIPKGTRDFAKEQMAIREKAFSIITSVFKRHGATALDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFA 407

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   136 RYVAMNNIQSIKRYHIAKVYRRDQPamTKGRMREFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKI 215
Cdd:PLN02972 408 RYVAMNGITSFKRYQIAKVYRRDNP--SKGRYREFYQCDFDIAGVYEPMGPDFEIIKVLTELLDELDIGTYEVKLNHRKL 485

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   216 LDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSA-DANITSNEK 294
Cdd:PLN02972 486 LDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSNETADKIGNFVKERGPPLELLSKLRQeGSEFLGNAS 565

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   295 AKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaselkkkAKSAEdasefvgVGSIAAG 374
Cdd:PLN02972 566 SRAALDELEIMFKALEKSKAIGKIVFDLSLARGLDYYTGVIYEAV----------------FKGAQ-------VGSIAAG 622

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   375 GRYDNLVNMFseaSGKkstQIPCVGISFGVERIFSLI-KQRINSSTTIKPTATQVFVMAFGGGKdwtgyLPERMKVTKQL 453
Cdd:PLN02972 623 GRYDNLVGMF---SGK---QVPAVGVSLGIERVFAIMeQQEEEKSQVIRPTETEVLVSIIGDDK-----LALAAELVSEL 691

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|..
gi 6325290   454 WDAGIEAEYvykaKANPRKQ--FDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:PLN02972 692 WNAGIKAEY----KVSTRKAkhLKRAKESGIPWMVLVGEKELSKGFVKLKNL 739
HisS COG0124
Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Histidyl-tRNA ...
 54-530 2.69e-105

Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Histidyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439894 [Multi-domain]  Cd Length: 422  Bit Score: 322.46  E-value: 2.69e-105
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   54 SLKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED--SKLIYNLEDQGGELCSLRYDLT 131
Cdd:COG0124   3 KIQAPRGTRDILPEESAKWQYVEDTIREVFERYGFQEIRTPIFEYTELFARKIGEDivEKEMYTFEDRGGRSLTLRPEGT 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  132 VPFARYVAMNNIQS---IKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKI 208
Cdd:COG0124  83 APVARAVAEHGNELpfpFKLYYIGPVFRYERPQ--KGRYRQFHQFGVEVIGS-DSPLADAEVIALAADLLKALGLKDFTL 159

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  209 KLNHRkildgifqiaGVKDEDVRKISSAVDKLDKSPWEAVkkeMTEEKgQSEETADKIGEYVKLNGSlkEIHAVLsADAN 288
Cdd:COG0124 160 EINSR----------GLPEERAEALLRYLDKLDKIGHEDV---LDEDS-QRRLETNPLRAILDSKGP--DCQEVL-ADAP 222

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  289 ITSNEKAKQGLDDIATLMKYTEAFDIDsfISFDLSLARGLDYYTGLIYEVVTsasappenaselkkkaksaedaSEFVGV 368
Cdd:COG0124 223 KLLDYLGEEGLAHFEEVLELLDALGIP--YVIDPRLVRGLDYYTGTVFEIVT----------------------DGLGAQ 278

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  369 GSIAAGGRYDNLVNMFseasGKKSTqiPCVGISFGVERIFSLIKQRinSSTTIKPTATQVFVMAFGGgkdwtGYLPERMK 448
Cdd:COG0124 279 GSVCGGGRYDGLVEQL----GGPPT--PAVGFAIGLERLLLLLEEL--GLLPAAEPPPDVYVVPLGE-----EARAEALK 345

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  449 VTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLgqefADDDGELVSAADIVPIVQEKL 528
Cdd:COG0124 346 LAQELRAAGIRVELDLGGR-KLKKQLKYADKSGAPFVLILGEDELANGTVTLKDL----ATGEQETVPLDELVEYLKELL 420


                ..
gi 6325290  529 SQ 530
Cdd:COG0124 421 AE 422
HisRS-like_core cd00773
Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain. HisRS is a homodimer. ...
 68-412 1.11e-99

Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain. HisRS is a homodimer. It is responsible for the attachment of histidine to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of the enzyme bound aminoacyl-adenylate. Class II assignment is based upon its structure and the presence of three characteristic sequence motifs. This domain is also found at the C-terminus of eukaryotic GCN2 protein kinase and at the N-terminus of the ATP phosphoribosyltransferase accessory subunit, HisZ. HisZ along with HisG catalyze the first reaction in histidine biosynthesis. HisZ is found only in a subset of bacteria and differs from HisRS in lacking a C-terminal anti-codon binding domain.


Pssm-ID: 238396 [Multi-domain]  Cd Length: 261  Bit Score: 302.21  E-value: 1.11e-99
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   68 DMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED-SKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNI--- 143
Cdd:cd00773   1 EAALRRYIEDTLREVFERYGYEEIDTPVFEYTELFLRKSGDEvSKEMYRFKDKGGRDLALRPDLTAPVARAVAENLLslp 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  144 QSIKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFqia 223
Cdd:cd00773  81 LPLKLYYIGPVFRYERPQ--KGRYREFYQVGVEIIGS-DSPLADAEVIALAVEILEALGLKDFQIKINHRGILDGIA--- 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  224 GVKDEDVRKISSAVDKLDKSpweavkkemteekgqseetadkigeyvklngslkeihavlsadanitsnekakqGLDDIA 303
Cdd:cd00773 155 GLLEDREEYIERLIDKLDKE------------------------------------------------------ALAHLE 180

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  304 TLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAppenaselkkkaksaedasefvGVGSIAAGGRYDNLVNM 383
Cdd:cd00773 181 KLLDYLEALGVDIKYSIDLSLVRGLDYYTGIVFEAVADGLG----------------------AQGSIAGGGRYDGLLEE 238

                       330       340
                ....*....|....*....|....*....
gi 6325290  384 FSeasgkkSTQIPCVGISFGVERIFSLIK 412
Cdd:cd00773 239 FG------GEDVPAVGFAIGLERLLLALE 261
hisS TIGR00442
histidyl-tRNA synthetase; This model finds a histidyl-tRNA synthetase in every completed ...
 56-503 4.02e-96

histidyl-tRNA synthetase; This model finds a histidyl-tRNA synthetase in every completed genome. Apparent second copies from Bacillus subtilis, Synechocystis sp., and Aquifex aeolicus are slightly shorter, more closely related to each other than to other hisS proteins, and actually serve as regulatory subunits for an enzyme of histidine biosynthesis. They were excluded from the seed alignment and score much lower than do single copy histidyl-tRNA synthetases of other genomes not included in the seed alignment. These putative second copies of HisS score below the trusted cutoff. The regulatory protein kinase GCN2 of Saccharomyces cerevisiae (YDR283c), and related proteins from other species designated eIF-2 alpha kinase, have a domain closely related to histidyl-tRNA synthetase that may serve to detect and respond to uncharged tRNA(his), an indicator of amino acid starvation; these regulatory proteins are not orthologous and so score below the noise cutoff. [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 273080 [Multi-domain]  Cd Length: 404  Bit Score: 297.85  E-value: 4.02e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290     56 KTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDS----KLIYNLEDQGGELCSLRYDLT 131
Cdd:TIGR00442   1 QKPRGTRDFLPEEMIKWQYIEETIREVFERYGFKEIRTPIFEYTELFKRKVGEETdivsKEMYTFKDKGGRSLTLRPEGT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    132 VPFARYVAMNNI---QSIKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKI 208
Cdd:TIGR00442  81 APVARAVIENKLllpKPFKLYYIGPMFRYERPQ--KGRYRQFHQFGVEVIGS-DSPLADAEIIALAADILKELGLKDFTL 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    209 KLNHRKILDGIFqiagvkdEDVRKISSAVDK-LDKSPWEAVKKEMTEEKGQSEETADKIGEYVKlngSLKEIHAVLSADa 287
Cdd:TIGR00442 158 EINSLGILEGRL-------EYREALIRYLDKhKDKLGEDSVRRLEKNPLRILDSKNEKIQELLK---NAPKILDFLCEE- 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    288 nitsnekakqGLDDIATLMKYTEAFDIDsfISFDLSLARGLDYYTGLIYEVVTsasappenaSELKKKaksaedasefvg 367
Cdd:TIGR00442 227 ----------SRAHFEELKELLDALGIP--YKIDPSLVRGLDYYTGTVFEFVT---------DDLGAQ------------ 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    368 vGSIAAGGRYDNLVNMFseaSGKKstqIPCVGISFGVERIFSLIKQriNSSTTIKPTATQVFVMAFGggkdwTGYLPERM 447
Cdd:TIGR00442 274 -GSICGGGRYDGLVEEL---GGPP---TPAVGFAIGIERLILLLEE--LGLIPPPSKKPDVYVVPLG-----EEAELEAL 339

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 6325290    448 KVTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:TIGR00442 340 KLAQKLRKAGIRVEVDLGGR-KLKKQLKYADKLGARFALIIGEDELENGTVTLKDL 394
tRNA-synt_His pfam13393
Histidyl-tRNA synthetase; This is a family of class II aminoacyl-tRNA synthetase-like and ATP ...
 72-407 8.57e-41

Histidyl-tRNA synthetase; This is a family of class II aminoacyl-tRNA synthetase-like and ATP phosphoribosyltransferase regulatory subunits.


Pssm-ID: 433170 [Multi-domain]  Cd Length: 309  Bit Score: 149.66  E-value: 8.57e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290     72 REAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFARYVA-MNNIQSIKR-Y 149
Cdd:pfam13393  13 IEELRRRLLDLFRSWGYELVMPPLLEYLDSLLTGTGADLDQTFKLVDQSGRLLGLRADITPQVARIDAhRLNRPGPLRlC 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    150 HIAKVYRRDQPAMtkGRMREFYQCD---FDVAGTfESmvpDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVK 226
Cdd:pfam13393  93 YAGSVLRTRPKGL--GRSREPLQVGaelIGHAGI-EA---DAEIISLLLEALAAAGVPGVTLDLGHVGLVRALLEAAGLS 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    227 DEDVRKISSAVDKLDkspWEAVKkEMTEEKGQSEETADKIGEYVKLNGslkEIHAVLSADANITSNEKAKQGLDDIATLM 306
Cdd:pfam13393 167 EALEEALRAALQRKD---AAELA-ELAAEAGLPPALRRALLALPDLYG---GPEVLDEARAALPGLPALQEALDELEALA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    307 KYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaselkkkAKSAEDAsefvgvgsIAAGGRYDNLVNMFSE 386
Cdd:pfam13393 240 ALLEALGDGVRLTFDLAELRGYEYYTGIVFAAY----------------APGVGEP--------LARGGRYDDLGAAFGR 295

                         330       340
                  ....*....|....*....|.
gi 6325290    387 AsgkkstqIPCVGISFGVERI 407
Cdd:pfam13393 296 A-------RPATGFSLDLEAL 309
 
Name Accession Description Interval E-value
PLN02972 PLN02972
Histidyl-tRNA synthetase
 56-503 6.33e-165

Histidyl-tRNA synthetase


Pssm-ID: 215525 [Multi-domain]  Cd Length: 763  Bit Score: 487.09  E-value: 6.33e-165
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    56 KTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFA 135
Cdd:PLN02972 328 KIPKGTRDFAKEQMAIREKAFSIITSVFKRHGATALDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFA 407

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   136 RYVAMNNIQSIKRYHIAKVYRRDQPamTKGRMREFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKI 215
Cdd:PLN02972 408 RYVAMNGITSFKRYQIAKVYRRDNP--SKGRYREFYQCDFDIAGVYEPMGPDFEIIKVLTELLDELDIGTYEVKLNHRKL 485

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   216 LDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSA-DANITSNEK 294
Cdd:PLN02972 486 LDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSNETADKIGNFVKERGPPLELLSKLRQeGSEFLGNAS 565

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   295 AKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaselkkkAKSAEdasefvgVGSIAAG 374
Cdd:PLN02972 566 SRAALDELEIMFKALEKSKAIGKIVFDLSLARGLDYYTGVIYEAV----------------FKGAQ-------VGSIAAG 622

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   375 GRYDNLVNMFseaSGKkstQIPCVGISFGVERIFSLI-KQRINSSTTIKPTATQVFVMAFGGGKdwtgyLPERMKVTKQL 453
Cdd:PLN02972 623 GRYDNLVGMF---SGK---QVPAVGVSLGIERVFAIMeQQEEEKSQVIRPTETEVLVSIIGDDK-----LALAAELVSEL 691

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|..
gi 6325290   454 WDAGIEAEYvykaKANPRKQ--FDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:PLN02972 692 WNAGIKAEY----KVSTRKAkhLKRAKESGIPWMVLVGEKELSKGFVKLKNL 739
HisS COG0124
Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Histidyl-tRNA ...
 54-530 2.69e-105

Histidyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Histidyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439894 [Multi-domain]  Cd Length: 422  Bit Score: 322.46  E-value: 2.69e-105
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   54 SLKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED--SKLIYNLEDQGGELCSLRYDLT 131
Cdd:COG0124   3 KIQAPRGTRDILPEESAKWQYVEDTIREVFERYGFQEIRTPIFEYTELFARKIGEDivEKEMYTFEDRGGRSLTLRPEGT 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  132 VPFARYVAMNNIQS---IKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKI 208
Cdd:COG0124  83 APVARAVAEHGNELpfpFKLYYIGPVFRYERPQ--KGRYRQFHQFGVEVIGS-DSPLADAEVIALAADLLKALGLKDFTL 159

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  209 KLNHRkildgifqiaGVKDEDVRKISSAVDKLDKSPWEAVkkeMTEEKgQSEETADKIGEYVKLNGSlkEIHAVLsADAN 288
Cdd:COG0124 160 EINSR----------GLPEERAEALLRYLDKLDKIGHEDV---LDEDS-QRRLETNPLRAILDSKGP--DCQEVL-ADAP 222

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  289 ITSNEKAKQGLDDIATLMKYTEAFDIDsfISFDLSLARGLDYYTGLIYEVVTsasappenaselkkkaksaedaSEFVGV 368
Cdd:COG0124 223 KLLDYLGEEGLAHFEEVLELLDALGIP--YVIDPRLVRGLDYYTGTVFEIVT----------------------DGLGAQ 278

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  369 GSIAAGGRYDNLVNMFseasGKKSTqiPCVGISFGVERIFSLIKQRinSSTTIKPTATQVFVMAFGGgkdwtGYLPERMK 448
Cdd:COG0124 279 GSVCGGGRYDGLVEQL----GGPPT--PAVGFAIGLERLLLLLEEL--GLLPAAEPPPDVYVVPLGE-----EARAEALK 345

                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  449 VTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLgqefADDDGELVSAADIVPIVQEKL 528
Cdd:COG0124 346 LAQELRAAGIRVELDLGGR-KLKKQLKYADKSGAPFVLILGEDELANGTVTLKDL----ATGEQETVPLDELVEYLKELL 420


                ..
gi 6325290  529 SQ 530
Cdd:COG0124 421 AE 422
HisRS-like_core cd00773
Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain. HisRS is a homodimer. ...
 68-412 1.11e-99

Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain. HisRS is a homodimer. It is responsible for the attachment of histidine to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of the enzyme bound aminoacyl-adenylate. Class II assignment is based upon its structure and the presence of three characteristic sequence motifs. This domain is also found at the C-terminus of eukaryotic GCN2 protein kinase and at the N-terminus of the ATP phosphoribosyltransferase accessory subunit, HisZ. HisZ along with HisG catalyze the first reaction in histidine biosynthesis. HisZ is found only in a subset of bacteria and differs from HisRS in lacking a C-terminal anti-codon binding domain.


Pssm-ID: 238396 [Multi-domain]  Cd Length: 261  Bit Score: 302.21  E-value: 1.11e-99
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   68 DMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED-SKLIYNLEDQGGELCSLRYDLTVPFARYVAMNNI--- 143
Cdd:cd00773   1 EAALRRYIEDTLREVFERYGYEEIDTPVFEYTELFLRKSGDEvSKEMYRFKDKGGRDLALRPDLTAPVARAVAENLLslp 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  144 QSIKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFqia 223
Cdd:cd00773  81 LPLKLYYIGPVFRYERPQ--KGRYREFYQVGVEIIGS-DSPLADAEVIALAVEILEALGLKDFQIKINHRGILDGIA--- 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  224 GVKDEDVRKISSAVDKLDKSpweavkkemteekgqseetadkigeyvklngslkeihavlsadanitsnekakqGLDDIA 303
Cdd:cd00773 155 GLLEDREEYIERLIDKLDKE------------------------------------------------------ALAHLE 180

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  304 TLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAppenaselkkkaksaedasefvGVGSIAAGGRYDNLVNM 383
Cdd:cd00773 181 KLLDYLEALGVDIKYSIDLSLVRGLDYYTGIVFEAVADGLG----------------------AQGSIAGGGRYDGLLEE 238

                       330       340
                ....*....|....*....|....*....
gi 6325290  384 FSeasgkkSTQIPCVGISFGVERIFSLIK 412
Cdd:cd00773 239 FG------GEDVPAVGFAIGLERLLLALE 261
hisS TIGR00442
histidyl-tRNA synthetase; This model finds a histidyl-tRNA synthetase in every completed ...
 56-503 4.02e-96

histidyl-tRNA synthetase; This model finds a histidyl-tRNA synthetase in every completed genome. Apparent second copies from Bacillus subtilis, Synechocystis sp., and Aquifex aeolicus are slightly shorter, more closely related to each other than to other hisS proteins, and actually serve as regulatory subunits for an enzyme of histidine biosynthesis. They were excluded from the seed alignment and score much lower than do single copy histidyl-tRNA synthetases of other genomes not included in the seed alignment. These putative second copies of HisS score below the trusted cutoff. The regulatory protein kinase GCN2 of Saccharomyces cerevisiae (YDR283c), and related proteins from other species designated eIF-2 alpha kinase, have a domain closely related to histidyl-tRNA synthetase that may serve to detect and respond to uncharged tRNA(his), an indicator of amino acid starvation; these regulatory proteins are not orthologous and so score below the noise cutoff. [Protein synthesis, tRNA aminoacylation]


Pssm-ID: 273080 [Multi-domain]  Cd Length: 404  Bit Score: 297.85  E-value: 4.02e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290     56 KTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDS----KLIYNLEDQGGELCSLRYDLT 131
Cdd:TIGR00442   1 QKPRGTRDFLPEEMIKWQYIEETIREVFERYGFKEIRTPIFEYTELFKRKVGEETdivsKEMYTFKDKGGRSLTLRPEGT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    132 VPFARYVAMNNI---QSIKRYHIAKVYRRDQPAmtKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKI 208
Cdd:TIGR00442  81 APVARAVIENKLllpKPFKLYYIGPMFRYERPQ--KGRYRQFHQFGVEVIGS-DSPLADAEIIALAADILKELGLKDFTL 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    209 KLNHRKILDGIFqiagvkdEDVRKISSAVDK-LDKSPWEAVKKEMTEEKGQSEETADKIGEYVKlngSLKEIHAVLSADa 287
Cdd:TIGR00442 158 EINSLGILEGRL-------EYREALIRYLDKhKDKLGEDSVRRLEKNPLRILDSKNEKIQELLK---NAPKILDFLCEE- 226

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    288 nitsnekakqGLDDIATLMKYTEAFDIDsfISFDLSLARGLDYYTGLIYEVVTsasappenaSELKKKaksaedasefvg 367
Cdd:TIGR00442 227 ----------SRAHFEELKELLDALGIP--YKIDPSLVRGLDYYTGTVFEFVT---------DDLGAQ------------ 273

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    368 vGSIAAGGRYDNLVNMFseaSGKKstqIPCVGISFGVERIFSLIKQriNSSTTIKPTATQVFVMAFGggkdwTGYLPERM 447
Cdd:TIGR00442 274 -GSICGGGRYDGLVEEL---GGPP---TPAVGFAIGIERLILLLEE--LGLIPPPSKKPDVYVVPLG-----EEAELEAL 339

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 6325290    448 KVTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:TIGR00442 340 KLAQKLRKAGIRVEVDLGGR-KLKKQLKYADKLGARFALIIGEDELENGTVTLKDL 394
PRK12420 PRK12420
histidyl-tRNA synthetase; Provisional
 55-508 1.13e-71

histidyl-tRNA synthetase; Provisional


Pssm-ID: 237097 [Multi-domain]  Cd Length: 423  Bit Score: 235.01  E-value: 1.13e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    55 LKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYG---EDSKLIYNLEDQGGELCSLRYDLT 131
Cdd:PRK12420   4 MRNVKGTKDYLPEEQVLRNKIKRALEDVFERYGCKPLETPTLNMYELMSSKYGggdEILKEIYTLTDQGKRDLALRYDLT 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   132 VPFARYVAMN-NI-QSIKRYHIAKVYrRDQPaMTKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIkDFKIK 209
Cdd:PRK12420  84 IPFAKVVAMNpNIrLPFKRYEIGKVF-RDGP-IKQGRFREFIQCDVDIVGV-ESVMAEAELMSMAFELFRRLNL-EVTIQ 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   210 LNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMTeEKGQSEETADKIGEYVKLNGSLKeihavLSADANI 289
Cdd:PRK12420 160 YNNRKLLNGILQAIGIPTELTSDVILSLDKIEKIGIDGVRKDLL-ERGISEEMADTICNTVLSCLQLS-----IADFKEA 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   290 TSNEKAKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaseLKKKAKSAedasefvgvg 369
Cdd:PRK12420 234 FNNPLVAEGVNELQQLQQYLIALGINENCIFNPFLARGLTMYTGTVYEIF------------LKDGSITS---------- 291

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   370 SIAAGGRYDNLVNMFSEasgkKSTQIPCVGISFGVERIFSLIKQRinssTTIKPTAtQVFVMAFGGGKdwtgylpERMKV 449
Cdd:PRK12420 292 SIGSGGRYDNIIGAFRG----DDMNYPTVGISFGLDVIYTALSQK----ETISSTA-DVFIIPLGTEL-------QCLQI 355

                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 6325290   450 TKQLW-DAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRL--GQEFA 508
Cdd:PRK12420 356 AQQLRsTTGLKVELELAGR-KLKKALNYANKENIPYVLIIGEEEVSTGTVMLRNMkeGSEVK 416
PLN02530 PLN02530
histidine-tRNA ligase
 46-514 9.13e-58

histidine-tRNA ligase


Pssm-ID: 178145 [Multi-domain]  Cd Length: 487  Bit Score: 200.35  E-value: 9.13e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    46 PKKGKLQVSLKTPKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED-SKLIYNLEDQGGELC 124
Cdd:PLN02530  61 QEDGKPKIDVNPPKGTRDFPPEDMRLRNWLFDHFREVSRLFGFEEVDAPVLESEELYIRKAGEEiTDQLYNFEDKGGRRV 140

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   125 SLRYDLTVPFARYVaMNNIQSI----KRYHIAKVYRRDQpaMTKGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTS 200
Cdd:PLN02530 141 ALRPELTPSLARLV-LQKGKSLslplKWFAIGQCWRYER--MTRGRRREHYQWNMDIIGV-PGVEAEAELLAAIVTFFKR 216

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   201 LGI--KDFKIKLNHRKILDGIFQIAGVKDEDVRKISSAVDKLDKSPWEAVKKEMtEEKGQSEETADKIGEyVKLNGSLKE 278
Cdd:PLN02530 217 VGItsSDVGIKVSSRKVLQAVLKSYGIPEESFAPVCVIVDKLEKLPREEIEKEL-DTLGVSEEAIEGILD-VLSLKSLDD 294

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   279 IHAVLSADAnitsnekakQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappENASELKkkaks 358
Cdd:PLN02530 295 LEALLGADS---------EAVADLKQLFSLAEAYGYQDWLVFDASVVRGLAYYTGIVFEGF-------DRAGKLR----- 353

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   359 aedasefvgvgSIAAGGRYDNLVNMFSeasgkkSTQIPCVGISFGVERIFSLIKQRinSSTTIKPTATQVFVMAFGGgkd 438
Cdd:PLN02530 354 -----------AICGGGRYDRLLSTFG------GEDTPACGFGFGDAVIVELLKEK--GLLPELPHQVDDVVFALDE--- 411

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6325290   439 wtGYLPERMKVTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVK--RLGQEFADDDGEL 514
Cdd:PLN02530 412 --DLQGAAAGVASRLREKGRSVDLVLEPK-KLKWVFKHAERIGAKRLVLVGASEWERGMVRVKdlSSGEQTEVKLDEL 486
hisZ_biosyn_reg TIGR00443
ATP phosphoribosyltransferase, regulatory subunit; Apparant second copies of histidyl-tRNA ...
 72-412 4.34e-50

ATP phosphoribosyltransferase, regulatory subunit; Apparant second copies of histidyl-tRNA synthetase, found in Bacillus subtilis, Synechocystis sp., Aquifex aeolicus, and others, are in fact a regulatory subunit of ATP phosphoribosyltransferase, and usually encoded by a gene adjacent to that encoding the catalytic subunit. [Amino acid biosynthesis, Histidine family]


Pssm-ID: 273081 [Multi-domain]  Cd Length: 313  Bit Score: 174.72  E-value: 4.34e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290     72 REAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFARYVA--MNNIQSIKR- 148
Cdd:TIGR00443  11 KEEIERQLQDVFRSWGYQEIITPTLEYLDTLSAGSGILNEDLFKLFDQLGRVLGLRPDMTAPIARLVStrLRDRPLPLRl 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    149 YHIAKVYRRDQPAMtkGRMREFYQCDFDVAGTfESMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVKDE 228
Cdd:TIGR00443  91 CYAGNVFRTNESGG--GRSREFTQAGVELIGA-GGPAADAEVIALLIEALKALGLKDFKIELGHVGLVRALLEEAGLPEE 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    229 DVRKISSAVDKLDKSpweAVKkEMTEEKGQSEETADKIGEYVKLNGSLKEihaVLSADANITSNEKAKQGLDDIATLMKY 308
Cdd:TIGR00443 168 AREALREALARKDLV---ALE-ELVAELGLSPEVRERLLALPRLRGDGEE---VLEEARALAGSETAEAALDELEAVLEL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    309 TEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSasappenaselkkkaksaedasefvGVG-SIAAGGRYDNLVNMFSEA 387
Cdd:TIGR00443 241 LEARGVEEYISLDLGLVRGYHYYTGLIFEGYAP-------------------------GLGaPLAGGGRYDELLGRFGRP 295

                         330       340
                  ....*....|....*....|....*
gi 6325290    388 sgkkstqIPCVGISFGVERIFSLIK 412
Cdd:TIGR00443 296 -------LPATGFALNLERLLEALT 313
HisZ COG3705
ATP phosphoribosyltransferase regulatory subunit HisZ [Amino acid transport and metabolism];
72-414 1.14e-48

ATP phosphoribosyltransferase regulatory subunit HisZ [Amino acid transport and metabolism];


Pssm-ID: 442919 [Multi-domain]  Cd Length: 312  Bit Score: 170.74  E-value: 1.14e-48
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   72 REAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKL-IYNLEDQGGELCSLRYDLTVPFARYVA--MNNIQSIKR 148
Cdd:COG3705   8 KEELRRRLLDVFRSWGYELVEPPLLEYLDSLLTGSGADLDLqTFKLVDQLGRTLGLRPDMTPQVARIAAtrLANRPGPLR 87

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  149 Y-HIAKVYRRdQPAMTkGRMREFYQCD---FDVAGTfESmvpDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAG 224
Cdd:COG3705  88 LcYAGNVFRT-RPSGL-GRSREFLQAGaelIGHAGL-EA---DAEVIALALEALKAAGLEDFTLDLGHVGLFRALLEALG 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  225 VKDEDVRKISSAVDKLDKSpweAVKkEMTEEKGQSEETADKIGEYVKLNGSlkeiHAVLSADANITSNEKAKQGLDDIAT 304
Cdd:COG3705 162 LSEEQREELRRALARKDAV---ELE-ELLAELGLSEELAEALLALPELYGG----EEVLARARALLLDAAIRAALDELEA 233

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  305 LMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPenaselkkkaksaedasefvgvgsIAAGGRYDNLVNMF 384
Cdd:COG3705 234 LAEALAARGPDVRLTFDLSELRGYDYYTGIVFEAYAPGVGDP------------------------LARGGRYDGLLAAF 289

                       330       340       350
                ....*....|....*....|....*....|
gi 6325290  385 SEAsgkkstqIPCVGISFGVERIFSLIKQR 414
Cdd:COG3705 290 GRA-------RPATGFSLDLDRLLRALPAA 312
hisZ PRK12292
ATP phosphoribosyltransferase regulatory subunit; Provisional
 58-493 3.12e-41

ATP phosphoribosyltransferase regulatory subunit; Provisional


Pssm-ID: 237043 [Multi-domain]  Cd Length: 391  Bit Score: 153.10  E-value: 3.12e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    58 PKGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREIL--AGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFA 135
Cdd:PRK12292   6 PEGIRDLLPEEARKIEEIRRRLLDLFRRWGYEEVITPTLEYLDTLlaGGGAILDLRTFKLVDQLSGRTLGLRPDMTAQIA 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   136 RYVA--MNNIQSIKR-YHIAKVYRRdqPAMTKGRMREFYQCDFDVAGtFESMVPDSECLSILVEGLTSLGIKDFKIKLNH 212
Cdd:PRK12292  86 RIAAtrLANRPGPLRlCYAGNVFRA--QERGLGRSREFLQSGVELIG-DAGLEADAEVILLLLEALKALGLPNFTLDLGH 162

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   213 RKILDGIFQIAGVKDEDVRKISSAVDKLDKSpweAVKKEmteEKGQSEETADKIGEYVKLNGSLKeihaVLSADANITSN 292
Cdd:PRK12292 163 VGLFRALLEAAGLSEELEEVLRRALANKDYV---ALEEL---VLDLSEELRDALLALPRLRGGRE----VLEEARKLLPS 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   293 EKAKQGLDDIATLMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSasappenaselkkkaksaedasefvGVG-SI 371
Cdd:PRK12292 233 LPIKRALDELEALAEALEKYGYGIPLSLDLGLLRHLDYYTGIVFEGYVD-------------------------GVGnPI 287

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   372 AAGGRYDNLVNMFSEAsgkkstqIPCVGISFGVERIFSLIKQrinssttIKPTATQVFVMAFgggkdWTGYLPERMKVTK 451
Cdd:PRK12292 288 ASGGRYDDLLGRFGRA-------RPATGFSLDLDRLLELQLE-------LPVEARKDLVIAP-----DSEALAAALAAAQ 348

                        410       420       430       440
                 ....*....|....*....|....*....|....*....|..
gi 6325290   452 QLWDAGIEAEYVYkakanPRKQFDAAEKAGCHIAVILGKEEY 493
Cdd:PRK12292 349 ELRKKGEIVVLAL-----PGRNFEDAREYARDRQIVEADGEW 385
tRNA-synt_His pfam13393
Histidyl-tRNA synthetase; This is a family of class II aminoacyl-tRNA synthetase-like and ATP ...
 72-407 8.57e-41

Histidyl-tRNA synthetase; This is a family of class II aminoacyl-tRNA synthetase-like and ATP phosphoribosyltransferase regulatory subunits.


Pssm-ID: 433170 [Multi-domain]  Cd Length: 309  Bit Score: 149.66  E-value: 8.57e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290     72 REAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLIYNLEDQGGELCSLRYDLTVPFARYVA-MNNIQSIKR-Y 149
Cdd:pfam13393  13 IEELRRRLLDLFRSWGYELVMPPLLEYLDSLLTGTGADLDQTFKLVDQSGRLLGLRADITPQVARIDAhRLNRPGPLRlC 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    150 HIAKVYRRDQPAMtkGRMREFYQCD---FDVAGTfESmvpDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGVK 226
Cdd:pfam13393  93 YAGSVLRTRPKGL--GRSREPLQVGaelIGHAGI-EA---DAEIISLLLEALAAAGVPGVTLDLGHVGLVRALLEAAGLS 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    227 DEDVRKISSAVDKLDkspWEAVKkEMTEEKGQSEETADKIGEYVKLNGslkEIHAVLSADANITSNEKAKQGLDDIATLM 306
Cdd:pfam13393 167 EALEEALRAALQRKD---AAELA-ELAAEAGLPPALRRALLALPDLYG---GPEVLDEARAALPGLPALQEALDELEALA 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    307 KYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVtsasappenaselkkkAKSAEDAsefvgvgsIAAGGRYDNLVNMFSE 386
Cdd:pfam13393 240 ALLEALGDGVRLTFDLAELRGYEYYTGIVFAAY----------------APGVGEP--------LARGGRYDDLGAAFGR 295

                         330       340
                  ....*....|....*....|.
gi 6325290    387 AsgkkstqIPCVGISFGVERI 407
Cdd:pfam13393 296 A-------RPATGFSLDLEAL 309
HisRS_anticodon cd00859
HisRS Histidyl-anticodon binding domain. HisRS belongs to class II aminoacyl-tRNA synthetases ...
 425-525 3.37e-20

HisRS Histidyl-anticodon binding domain. HisRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in tRNA-binding, so that the activated amino acid is transferred to a ribose 3' OH group of the appropriate tRNA only.


Pssm-ID: 238436 [Multi-domain]  Cd Length: 91  Bit Score: 85.28  E-value: 3.37e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290  425 ATQVFVMAFGGGkdwtgYLPERMKVTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLg 504
Cdd:cd00859   1 EVDVYVVPLGEG-----ALSEALELAEQLRDAGIKAEIDYGGR-KLKKQFKYADRSGARFAVILGEDELAAGVVTVKDL- 73

                        90       100
                ....*....|....*....|.
gi 6325290  505 qefADDDGELVSAADIVPIVQ 525
Cdd:cd00859  74 ---ETGEQETVALDELVEELK 91
syh CHL00201
histidine-tRNA synthetase; Provisional
 59-503 2.07e-19

histidine-tRNA synthetase; Provisional


Pssm-ID: 164576 [Multi-domain]  Cd Length: 430  Bit Score: 90.73  E-value: 2.07e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    59 KGTKDWADSDMVIREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGEDSKLI----YNLEDQGGELCSLRYDLTVPF 134
Cdd:CHL00201   8 RGTKDILPDEINYWQFIHDKALTLLSLANYSEIRTPIFENSSLYDRGIGETTDIVnkemYRFTDRSNRDITLRPEGTAGI 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   135 ARYVAMNNIQ---SIKR-YHIAKVYRRDQPamTKGRMREFYQCDFDVAGTFESMVpDSECLSILVEGLTSLGIKDFKIKL 210
Cdd:CHL00201  88 VRAFIENKMDyhsNLQRlWYSGPMFRYERP--QSGRQRQFHQLGIEFIGSIDARA-DTEVIHLAMQIFNELQVKNLILDI 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   211 NhrkildgifQIAGVKDEDVRK------ISSAVDKLDKSPweavKKEMTEEKGQSEETADKIGEYVkLNGSLKeIHAVLs 284
Cdd:CHL00201 165 N---------SIGKLEDRQSYQlklveyLSQYQDDLDTDS----QNRLYSNPIRILDSKNLKTQEI-LDGAPK-ISDFL- 228

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   285 adanitsNEKAKQGLDDIATlmkYTEAFDIDSFISFdlSLARGLDYYTgliyevvtsasappENASELKKKAKSAEDase 364
Cdd:CHL00201 229 -------SLESTEHFYDVCT---YLNLLNIPYKINY--KLVRGLDYYN--------------DTAFEIKTLSSNGQD--- 279

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   365 fvgvgSIAAGGRYDNLVNmfsEASGKKStqiPCVGISFGVERIFSLIKQRINssttIKPTATQVFVMAFGGGKDWTGylp 444
Cdd:CHL00201 280 -----TICGGGRYDSLIH---QLGGPKT---PAVGCAIGLERLLLIAKDNII----LPKQSIDVYIATQGLKAQKKG--- 341

                        410       420       430       440       450
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 6325290   445 erMKVTKQLWDAGIEAEyVYKAKANPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRL 503
Cdd:CHL00201 342 --WEIIQFLEKQNIKFE-LDLSSSNFHKQIKQAGKKRAKACIILGDNEIMDNCITIKWL 397
hisZ PRK12295
ATP phosphoribosyltransferase regulatory subunit; Provisional
 71-410 1.75e-18

ATP phosphoribosyltransferase regulatory subunit; Provisional


Pssm-ID: 183413 [Multi-domain]  Cd Length: 373  Bit Score: 87.30  E-value: 1.75e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    71 IREAIFSTLSGLFKKHGGVTIDTPVFELREILAGKYGED-SKLIYNLEDQ-GGELCsLRYDLTVPFAR-YVAMNNIQSIK 147
Cdd:PRK12295   6 ASAAAAEALLASFEAAGAVRVDPPILQPAEPFLDLSGEDiRRRIFVTSDEnGEELC-LRPDFTIPVCRrHIATAGGEPAR 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   148 RYHIAKVYRRdqpamTKGRMREFYQCDFDVAGTFESMVPDSECLSILVEGLTSLGIKDFKIKLN---------------- 211
Cdd:PRK12295  85 YAYLGEVFRQ-----RRDRASEFLQAGIESFGRADPAAADAEVLALALEALAALGPGDLEVRLGdvglfaalvdalglpp 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   212 ---------------------------------HRKILDGIFQIAGVKD--EDVRKISSAVDKLDKSPWE-----AVKKE 251
Cdd:PRK12295 160 gwkrrllrhfgrprsldallarlagprvdpldeHAGVLAALADEAAARAlvEDLMSIAGISPVGGRSPAEiarrlLEKAA 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   252 MTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANitsneKAKQGLD-DIATL---MKYTEAFDID-SFISFDLSLAR 326
Cdd:PRK12295 240 LAAAARLPAEALAVLERFLAISGPPDAALAALRALAA-----DAGLDLDaALDRFearLAALAARGIDlERLRFSASFGR 314

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   327 GLDYYTGLIYEVVTSASAPPenaselkkkaksaedasefvgvgSIAAGGRYDNLVNMFSEAsgkksTQIPCVGISFGVER 406
Cdd:PRK12295 315 PLDYYTGFVFEIRAAGNGDP-----------------------PLAGGGRYDGLLTRLGAG-----EPIPAVGFSIWLDR 366


                 ....
gi 6325290   407 IFSL 410
Cdd:PRK12295 367 LAAL 370
HGTP_anticodon pfam03129
Anticodon binding domain; This domain is found in histidyl, glycyl, threonyl and prolyl tRNA ...
 427-527 2.90e-11

Anticodon binding domain; This domain is found in histidyl, glycyl, threonyl and prolyl tRNA synthetases it is probably the anticodon binding domain.


Pssm-ID: 460819 [Multi-domain]  Cd Length: 94  Bit Score: 59.91  E-value: 2.90e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    427 QVFVMAFGGGKDwtGYLPERMKVTKQLWDAGIEAEYVYKAKaNPRKQFDAAEKAGCHIAVILGKEEYLEGKLRVKRLgqe 506
Cdd:pfam03129   1 QVVVIPLGEKAE--ELEEYAQKLAEELRAAGIRVELDDRNE-SIGKKFRRADLIGIPFALVVGEKELEEGTVTVRRR--- 74

                          90       100
                  ....*....|....*....|.
gi 6325290    507 fADDDGELVSAADIVPIVQEK 527
Cdd:pfam03129  75 -DTGEQETVSLDELVEKLKEL 94
PRK12421 PRK12421
ATP phosphoribosyltransferase regulatory subunit; Provisional
 82-378 6.36e-10

ATP phosphoribosyltransferase regulatory subunit; Provisional


Pssm-ID: 237098  Cd Length: 392  Bit Score: 61.14  E-value: 6.36e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290    82 LFKKHGGVTIDTPVFELREILAGKYGEDSKL-IYNLEDQ-GGELCSLRYDLTVPFARYVA-MNNIQSIKRY-HIAKVYRR 157
Cdd:PRK12421  34 LFASRGYQLVMPPLIEYLESLLTGAGQDLKLqTFKLIDQlSGRLMGVRADITPQVARIDAhLLNREGVARLcYAGSVLHT 113

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   158 DQPAMTKGRM-----REFYQCDfdvagtfeSMVPDSECLSILVEGLTSLGIKDFKIKLNHRKILDGIFQIAGV--KDEDV 230
Cdd:PRK12421 114 LPQGLFGSRTplqlgAELYGHA--------GIEADLEIIRLMLGLLRNAGVPALHLDLGHVGIFRRLAELAGLspEEEEE 185

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   231 ------RKissAVDKLdkspweavkKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADAniTSNEKAKQGLDDIAT 304
Cdd:PRK12421 186 lfdllqRK---ALPEL---------AEVCQNLGVGSDLRRMFYALARLNGGLEALDRALSVLA--LQDAAIRQALDELKT 251

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 6325290   305 LMKYTEAFDIDSFISFDLSLARGLDYYTGLIYEVVTSASAPPenaselkkkaksaedasefvgvgsIAAGGRYD 378
Cdd:PRK12421 252 LAAHLKNRWPELPVSIDLAELRGYHYHTGLVFAAYIPGRGQA------------------------LARGGRYD 301
class_II_aaRS-like_core cd00768
Class II tRNA amino-acyl synthetase-like catalytic core domain. Class II amino acyl-tRNA ...
 71-205 3.67e-05

Class II tRNA amino-acyl synthetase-like catalytic core domain. Class II amino acyl-tRNA synthetases (aaRS) share a common fold and generally attach an amino acid to the 3' OH of ribose of the appropriate tRNA. PheRS is an exception in that it attaches the amino acid at the 2'-OH group, like class I aaRSs. These enzymes are usually homodimers. This domain is primarily responsible for ATP-dependent formation of the enzyme bound aminoacyl-adenylate. The substrate specificity of this reaction is further determined by additional domains. Intererestingly, this domain is also found is asparagine synthase A (AsnA), in the accessory subunit of mitochondrial polymerase gamma and in the bacterial ATP phosphoribosyltransferase regulatory subunit HisZ.


Pssm-ID: 238391 [Multi-domain]  Cd Length: 211  Bit Score: 45.19  E-value: 3.67e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6325290   71 IREAIFSTLSGLFKKHGGVTIDTPVFELREiLAGKYGEDSKLIYNLEDQGGELCSLRYDLTvPFARYVAMNNIQSI--KR 148
Cdd:cd00768   1 IRSKIEQKLRRFMAELGFQEVETPIVEREP-LLEKAGHEPKDLLPVGAENEEDLYLRPTLE-PGLVRLFVSHIRKLplRL 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 6325290  149 YHIAKVYRRDQPAMTKGRMREFYQCDFDVAG-TFESMVPDSECLSILVEGLTSLGIKD 205
Cdd:cd00768  79 AEIGPAFRNEGGRRGLRRVREFTQLEGEVFGeDGEEASEFEELIELTEELLRALGIKL 136
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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