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Conserved domains on  [gi|940665250|gb|KQB34585|]
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transcriptional regulator [Acidiplasma aeolicum]

Protein Classification

TenA family protein( domain architecture ID 14427097)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 15-196 7.74e-55

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


:

Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 172.92  E-value: 7.74e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  15 IIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEA----GTKIKNNTKNNGIIELYDAIGsDEPVFHLQMLDKF 90
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARAlalaAAKAPDLELRTFLAELINVLD-DELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  91 GIKKENITEDLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAE---NSNVKSDKYSFWFDFYKSDGYIKITA 166
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPaEGLAALLPCYWSYGEIGRrlaASLPEHPPYRFWIDFYASDEYQELVE 159

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 940665250 167 KYFDILKDL-----ELTGMQKNIFKFGLLYELNYW 196
Cdd:cd16099  160 ELLQLLDQLaaageEEKEELKEIFLTSLRYELMFW 194
 
Name Accession Description Interval E-value
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 15-196 7.74e-55

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 172.92  E-value: 7.74e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  15 IIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEA----GTKIKNNTKNNGIIELYDAIGsDEPVFHLQMLDKF 90
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARAlalaAAKAPDLELRTFLAELINVLD-DELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  91 GIKKENITEDLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAE---NSNVKSDKYSFWFDFYKSDGYIKITA 166
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPaEGLAALLPCYWSYGEIGRrlaASLPEHPPYRFWIDFYASDEYQELVE 159

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 940665250 167 KYFDILKDL-----ELTGMQKNIFKFGLLYELNYW 196
Cdd:cd16099  160 ELLQLLDQLaaageEEKEELKEIFLTSLRYELMFW 194
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 10-196 1.05e-30

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 111.90  E-value: 1.05e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELY----DAIGSDEPVFHLQ 85
Cdd:COG0819    9 EAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLaglaAGLLEVELALHER 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  86 MLDKFGIKKENITEDLINYTTYSYINHLIRWSEDSDYNGML-SMLPCQWSY----EYIAENSNVKSDKYSFWFDFYKSDG 160
Cdd:COG0819   89 YAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLaALLPCEWGYaeigKRLAERPLPPDHPYAEWIETYASEE 168

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 940665250 161 YIKITAKYFDILKDL-------ELTGMqKNIFKFGLLYELNYW 196
Cdd:COG0819  169 FQELVEWLIALLDRLaataseaERERL-EEAFRTASRLEYAFW 210
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 10-202 2.20e-18

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 79.32  E-value: 2.20e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAIGSD----EPVFHLQ 85
Cdd:pfam03070   2 PQLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDhdgnEIELHLR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   86 MLDKFGIKKENITEDLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAEN-----SNVKSDKYSFWFDFYKSD 159
Cdd:pfam03070  82 LAEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWlEAVAALLPCLFVYQEIASRlgekiRALEGPEYYEWVKTYASE 161

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 940665250  160 GYIKITAKYFDILKDLELTGMQ------KNIFKFGLLYELNYWICSYNG 202
Cdd:pfam03070 162 YFRAAVEEAKRLLDRLLAYVSDeqleelQEIFLKALEFEYSFWTMALDA 210
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 12-200 1.72e-14

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 68.76  E-value: 1.72e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   12 IKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE----AGTKIKNNTKNNGIIELYDAIGSDEPVFHLQML 87
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKvhalGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   88 DKFGIKKENITEDLI-NYTTYSYINHLIRWSEDSDYNGML-SMLPCQWSYEYIAENSNVKSDK-----YSFWFDFYKSDG 160
Cdd:TIGR04306  81 EVLGISDEEYFQKIKpGPALRSYTSYMYDVSRRGNWQELVaALLPCLWGYGEAATKLKGKHTApehsvYHKWIETYSSSW 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 940665250  161 YIKITAKYFDILKDLELTGMQ------KNIFKFGLLYELNYWICSY 200
Cdd:TIGR04306 161 FREAVNEGENLLNHLAETSSPeeleklKTIFAESCEYEYNFWTMAY 206
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 14-167 2.48e-09

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 56.14  E-value: 2.48e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  14 GIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAIGSD----EPVFHLQMLDK 89
Cdd:PRK09517 556 DIIAEINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAEcivvEAELHRSYLSG 635

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  90 fgikkeNITEDLINYTTYSYINHLIRWSEDSDYN-GMLSMLPCQWSYEYIA---ENSNVKSDKYSFWFDFYKSDGYIKIT 165
Cdd:PRK09517 636 ------KEAPSAPSPVTMAYTDFLIARTYTEDYVvGVAAVLPCYWLYAEIGlmlAEQNHDEHPYKDWLNTYSGEEFIAGT 709


                 ..
gi 940665250 166 AK 167
Cdd:PRK09517 710 RA 711
 
Name Accession Description Interval E-value
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 15-196 7.74e-55

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 172.92  E-value: 7.74e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  15 IIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEA----GTKIKNNTKNNGIIELYDAIGsDEPVFHLQMLDKF 90
Cdd:cd16099    1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARAlalaAAKAPDLELRTFLAELINVLD-DELELHEKLLAEL 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  91 GIKKENITEDLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAE---NSNVKSDKYSFWFDFYKSDGYIKITA 166
Cdd:cd16099   80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPaEGLAALLPCYWSYGEIGRrlaASLPEHPPYRFWIDFYASDEYQELVE 159

                        170       180       190
                 ....*....|....*....|....*....|....*
gi 940665250 167 KYFDILKDL-----ELTGMQKNIFKFGLLYELNYW 196
Cdd:cd16099  160 ELLQLLDQLaaageEEKEELKEIFLTSLRYELMFW 194
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 10-196 1.05e-30

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 111.90  E-value: 1.05e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELY----DAIGSDEPVFHLQ 85
Cdd:COG0819    9 EAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLaglaAGLLEVELALHER 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  86 MLDKFGIKKENITEDLINYTTYSYINHLIRWSEDSDYNGML-SMLPCQWSY----EYIAENSNVKSDKYSFWFDFYKSDG 160
Cdd:COG0819   89 YAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLaALLPCEWGYaeigKRLAERPLPPDHPYAEWIETYASEE 168

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 940665250 161 YIKITAKYFDILKDL-------ELTGMqKNIFKFGLLYELNYW 196
Cdd:COG0819  169 FQELVEWLIALLDRLaataseaERERL-EEAFRTASRLEYAFW 210
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 24-196 6.37e-24

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 93.79  E-value: 6.37e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAIGSD----EPVFHLQMLDKFGIKKENITE 99
Cdd:cd19364   21 FIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGlaegELALRETFFKELGITEEEIAQ 100

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250 100 DLINYTTYSYINHLIRWSEDSDYNGML-SMLPCQWSYEYIAE-NSNVKSDK--YSFWFDFYKSDGYIKITAKYFDILKDL 175
Cdd:cd19364  101 TPPAPTAYHYVSHMYRQLNEGSVAEAVaALLPCYWLYQEIGErLADAGSPVplYQRWIDTYASDEFTESVQQQIDLVDRL 180

                        170       180
                 ....*....|....*....|....*...
gi 940665250 176 -------ELTGMqKNIFKFGLLYELNYW 196
Cdd:cd19364  181 aeeaseeEREKM-KQAFLISSYYELQFW 207
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 24-196 4.00e-20

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 83.82  E-value: 4.00e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE--AGTKIKNNTKNNgIIELYDAIG---SDEPVFHLQMLDKFGIKKENIT 98
Cdd:cd19369   11 FIKELGEGTLDKEKFKNYLIQDSLYLKEYAKvfAMGIYKSRTMKE-MQFFYSSLSfvnEDETATRIKYLKEFGLTDEDIE 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  99 EDLINYTTYSYINHLIRWSEDSD-YNGMLSMLPCQWSYEYIA------ENSNVKSDKYSFWFDFYKSDGYIKIT---AKY 168
Cdd:cd19369   90 KIEPLPENKAYTDYMLGIAKTGDvKEILMAVLPCMLSYYYIFkelvkkYKDNLESNPYKDWIEDYASEEYAEYCkewIDF 169

                        170       180       190
                 ....*....|....*....|....*....|.
gi 940665250 169 FDIL-KDLELTGMQK--NIFKFGLLYELNYW 196
Cdd:cd19369  170 ADRLcENLSEEEKEKlkEIFRKASLYELKFW 200
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 24-196 5.18e-20

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 83.78  E-value: 5.18e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELY----DAIGSDEPVFHLQMLDKFGIKKENITE 99
Cdd:cd19361   21 FVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFadliNAILNEEMDIHRGYMKRLGITEEEIEN 100

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250 100 DLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAEN------SNVKSDKYSFWFDFYKSDGYIKITAKYFDIL 172
Cdd:cd19361  101 TKPALDNLSYTSYMLSVAYEGGIaEILAAILSCSWSYEYIAKKlverypAALEHEFYGEWVKGYSSEEYAEANQELIDLL 180

                        170       180       190
                 ....*....|....*....|....*....|
gi 940665250 173 ----KDLELTGMQK--NIFKFGLLYELNYW 196
Cdd:cd19361  181 drltEDISEEQIEKleEIFVNCSRYELKFW 210
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 15-172 1.06e-18

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 80.33  E-value: 1.06e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  15 IIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLK-YYKEAGTKIKNNTKNNGIIELYDAIG---SDEPVFHLQMLDKF 90
Cdd:cd19360   12 ILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNaFLKVYALAIAKSDTREDMRFFLEQIGfilNGESHAHQNLCEVA 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  91 GIKKENITEDLINYTTYSYINHLIR-WSEDSDYNGMLSMLPCQWSYEYIA-----ENSNVKSDKYSFWFDFYKSDGYIKI 164
Cdd:cd19360   92 GVDYEELQGAPWAPTADHYIKHMYYaARTGDLGDILAALLPCPWTYVELAkrlieEGKPTPDNPFYEWIDFYADDEMDGL 171


                 ....*...
gi 940665250 165 TAKYFDIL 172
Cdd:cd19360  172 TDQLFARL 179
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 10-202 2.20e-18

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 79.32  E-value: 2.20e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAIGSD----EPVFHLQ 85
Cdd:pfam03070   2 PQLRPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDhdgnEIELHLR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   86 MLDKFGIKKENITEDLINYTTYSYINHLIRWSEDSDY-NGMLSMLPCQWSYEYIAEN-----SNVKSDKYSFWFDFYKSD 159
Cdd:pfam03070  82 LAEALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWlEAVAALLPCLFVYQEIASRlgekiRALEGPEYYEWVKTYASE 161

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 940665250  160 GYIKITAKYFDILKDLELTGMQ------KNIFKFGLLYELNYWICSYNG 202
Cdd:pfam03070 162 YFRAAVEEAKRLLDRLLAYVSDeqleelQEIFLKALEFEYSFWTMALDA 210
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 24-196 7.54e-18

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 77.87  E-value: 7.54e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAI--GSDEPV-FHLQMLDKFGIKKENITED 100
Cdd:cd19362   20 FVERMRDGTLPLDNFRYYLIQDSKYVEEMLRALLRASSKAPLDKAIKILNSVfsGRDKGMeVHKFLYSELGITEDEIRRT 99

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250 101 LINYTTYSYINHLIRWSEDSDYNGMLSMLPCQWSYEYIAENS-NVKSDKYSFWFDFYKSDGYIKITAKYFDILKDLELTG 179
Cdd:cd19362  100 GYNLVNYAYTRHLYYYSTLGWPQFLAAWAPCMWGYSEIGKYVlNSPNELYKTWASFYASKDYKKRVEAILEALDSIEDTE 179

                        170
                 ....*....|....*..
gi 940665250 180 MQKNIFKFGLLYELNYW 196
Cdd:cd19362  180 DIKNIFRNSVNFEIMFW 196
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 10-196 7.71e-18

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 77.75  E-value: 7.71e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYL----KYYKEAGTKIKNNTKNNGIIELYDAIGSDEPVFHLQ 85
Cdd:cd19363    7 RDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLvgstKNLSILASKAESLDLMRELLELAYGEATTEFANYEE 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  86 MLDKFGIKKENITEDLINYTTYSYINHLIRWS-EDSDYNGMLSMLPCQWSYEYIAEN-----SNVKSDKYSFWFDFYKSD 159
Cdd:cd19363   87 LLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSsLGSFYEGLAALLPCFWSYLEIAEYhkdklSENPNDIYRDWASVYLSK 166

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 940665250 160 GYIKITAKYFDILkDLELTGMQ----KNIFKFGLLYELNYW 196
Cdd:cd19363  167 EYKELVERLRRIV-DKYGEGEPfeklKRIFKTASKYEYMFW 206
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 10-171 2.46e-16

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 73.70  E-value: 2.46e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  10 DEIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELY-----DAIGSdEPVFHL 84
Cdd:cd19365    6 EAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFarfaaGAIEV-ERELHR 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  85 QMLDKFGIKKEniTEdlINYTTYSYINHLIRWSEDSDYN-GMLSMLPCQWSY----EYIAENsNVKSDKYSFWFDFYKSD 159
Cdd:cd19365   85 SFLGEFGIDAA--AE--PSPVTLAYTSFLLATAATGPYAvAVAAVLPCFWIYaevgKRLAAA-ASPNHPYQDWIDTYSDE 159

                        170
                 ....*....|..
gi 940665250 160 GYIKITAKYFDI 171
Cdd:cd19365  160 EFAEAVRRAIAI 171
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 11-196 2.81e-16

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 73.75  E-value: 2.81e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  11 EIKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE----AGTKIKNNTKNNGIIELYDAIGSDEPVFHLQM 86
Cdd:cd19366    8 KAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARvfalGAAKADDLETMGRFAELLHGTLNTEMDLHRQY 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  87 LDKFGIKKENITEDLINYTTYSYINHLIRWSedsdYNGML-----SMLPCQWSYEYI----AENSNVKSDK-YSFWFDFY 156
Cdd:cd19366   88 AAEFGITEEELEATEPSPTTLAYTSYMLRTA----QTGTLaellaALLPCAWGYAEIgkrlAEQGGALEHNpYREWIEMY 163

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 940665250 157 KSDGYIKITAKYFDILKDL--ELTGMQK----NIFKFGLLYELNYW 196
Cdd:cd19366  164 SSDEFTELADWLIDLLDELaeGKSEAELerleEIFLTSSRYEYMFW 209
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 12-200 1.72e-14

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 68.76  E-value: 1.72e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   12 IKGIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE----AGTKIKNNTKNNGIIELYDAIGSDEPVFHLQML 87
Cdd:TIGR04306   1 VKPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKvhalGGSKACDEDMEKELVEQIQGGVETEMGQHKRLA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250   88 DKFGIKKENITEDLI-NYTTYSYINHLIRWSEDSDYNGML-SMLPCQWSYEYIAENSNVKSDK-----YSFWFDFYKSDG 160
Cdd:TIGR04306  81 EVLGISDEEYFQKIKpGPALRSYTSYMYDVSRRGNWQELVaALLPCLWGYGEAATKLKGKHTApehsvYHKWIETYSSSW 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 940665250  161 YIKITAKYFDILKDLELTGMQ------KNIFKFGLLYELNYWICSY 200
Cdd:TIGR04306 161 FREAVNEGENLLNHLAETSSPeeleklKTIFAESCEYEYNFWTMAY 206
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 19-196 4.65e-10

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 56.59  E-value: 4.65e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  19 IMNAKFVLEMQDGSLSDDKFKYYLIQDDIYL----KYYKEAGTKIKNNTKNNGIIELYDAIGSDEPVFHLQMLDK----F 90
Cdd:cd19359   15 ALNNPFCQGMADGTLDLDGFGYYMVQDYYYCinyvRFKALRAAKAPDPDLLAFLAAKIKSYLDYAEDFLKTCHIKlgipD 94

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  91 GIKKENITEDLINYTtySYINHLIRwSEDSdYNGMLSMLPCQWSYEYIAE-----NSNVKSDKYSFWFD--FYKSDG--- 160
Cdd:cd19359   95 VVDGVKPSPALKAYV--DFERSVAE-SEDW-FYLLVAMIPCIYLWYWLANqlnedPSDKNTNFYKTWIEpnLPDPSSakq 170

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 940665250 161 YIKITAKYFDILKDLELTGMQknIFKFGLLYELNYW 196
Cdd:cd19359  171 LEFFLNANAAWSKIDREKANE--IFRQAMQLEINFF 204
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 14-167 2.48e-09

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 56.14  E-value: 2.48e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  14 GIIKNIMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYDAIGSD----EPVFHLQMLDK 89
Cdd:PRK09517 556 DIIAEINDSDFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAEcivvEAELHRSYLSG 635

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  90 fgikkeNITEDLINYTTYSYINHLIRWSEDSDYN-GMLSMLPCQWSYEYIA---ENSNVKSDKYSFWFDFYKSDGYIKIT 165
Cdd:PRK09517 636 ------KEAPSAPSPVTMAYTDFLIARTYTEDYVvGVAAVLPCYWLYAEIGlmlAEQNHDEHPYKDWLNTYSGEEFIAGT 709


                 ..
gi 940665250 166 AK 167
Cdd:PRK09517 710 RA 711
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 24-175 3.00e-08

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 51.74  E-value: 3.00e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE----AGTK------IKNNTKN-NGIIElydaigsdEPVFHLQMLDKFGI 92
Cdd:cd19367   11 FVRQLADGTLPLESFRHYLKQDYLYLIHYARahalAAYKaptledIRAAAAIlLAILE--------EMELHVKYCAEWGI 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  93 KK---ENITEDLinyTTYSYINHLIRWSEDSDYNGML-SMLPCQWSYEYIAEN------SNVKSDKYSFWFDFYKSDGYI 162
Cdd:cd19367   83 SEeelEATPESP---ATLAYTRYVLDVGLSGDLLDLLvALAPCLLGYGEIGLRlaadpaTKLEGNPYWSWIETYASDEYQ 159

                        170
                 ....*....|...
gi 940665250 163 KITAKYFDILKDL 175
Cdd:cd19367  160 EAVREGIELLEKL 172
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 24-196 5.31e-07

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 49.19  E-value: 5.31e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  24 FVLEMQDGSLSDDKFKYYLIQDDIYLKYYKE----AGTKIKNNTKNNGIIELYDAIGSDEPVFHLQMLDkfgikkeNITE 99
Cdd:PTZ00347  33 FVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRilsyCITKSDVTATGGGLLELLKGVLEELKNCHHHYID-------NPDA 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250 100 DLINYTTYSYINHLIRWSEDSDYNGML---SMLPCQWSYEYIAE---NSNVKSDKYSF--WFDFYkSDGYIKITAKYFDI 171
Cdd:PTZ00347 106 AGPEAACRKYVDFLLASGNADTLGPSVviaAVIPCARLYAWVGQeltNEVELTESHPFrrWLLSY-SDEPINTSVEQLES 184

                        170       180
                 ....*....|....*....|....*....
gi 940665250 172 LKDLEL-TGMQKNI---FKFGLLYELNYW 196
Cdd:PTZ00347 185 LLDKYIrPGEFSEVaqaYRRAMELEYDFF 213
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
19-167 1.63e-06

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 47.48  E-value: 1.63e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  19 IMNAKFVLEMQDGSLSDDKFKYYLIQDDIYLKYYKEAGTKIKNNTKNNGIIELYdAIGSD-----EPVFHLQMLDKFGIK 93
Cdd:PRK14713 335 IEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFW-AQSAQaclevESELHRSWLGDRDAD 413

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 940665250  94 KEnitedlINYTTYSYINHLIRWSEDSDYN-GMLSMLPCQWSYEYI-----AENSNVKSDKYSFWFDFYKSDGYIKITAK 167
Cdd:PRK14713 414 TA------PSPVTLAYTDFLLARAAGGSYAvGAAAVLPCFWLYAEVgaelhARAGNPDDHPYAEWLQTYADPEFAAATRR 487
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 21-45 1.23e-03

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 38.32  E-value: 1.23e-03
                         10        20
                 ....*....|....*....|....*
gi 940665250  21 NAKFVLEMQDGSLSDDKFKYYLIQD 45
Cdd:cd19358   18 THRFVRELCAGTLPDAVLARYLVQD 42
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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