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Conserved domains on  [gi|1886460095|gb|KAF6285642|]
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F-box protein 31 [Myotis myotis]

Protein Classification

F-box protein( domain architecture ID 18135585)

F-box protein is the substrate-recognition component of an SCF (Skp1-cullin-F-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins; similar to mammalian F-box only protein 31

CATH:  1.20.1280.50
Gene Ontology:  GO:0006511|GO:0019005|GO:0005515
PubMed:  31898225|11178263
SCOP:  4001927

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Cyclin_D1_bind pfam12014
Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with ...
 265-463 4.06e-67

Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with normal and tumour cell proliferation and survival. The best characterized member of this family is the SCF FBXO31 (Skp1-Cul1-Rbx1-FBXO31) ubiquitin ligase complex mediates genotoxic stress-induced cyclin D1 degradation. FBXO31 possesses a unique substrate-binding beta barrel domain, whereas cyclin D1 binds to FBXO31 by tucking its free C-terminal carboxylate tail into an open cavity of the C-terminal FBXO31 beta-barrel. Biophysical and functional studies demonstrated that SCFFBXO31 is capable of recruiting and ubiquitinating cyclin D1 in a phosphorylation-independent manner.


:

Pssm-ID: 463431  Cd Length: 147  Bit Score: 212.08  E-value: 4.06e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 265 YRRIYLPPShpddLIRP--GLFKGTYGSHGLEIVMLSF------HGKHARVTKITGDPNIPAGQQTVEIDlmhriqlpNI 336
Cdd:pfam12014   1 YRRLYLPPT----LIRPfrGLFVGDYGGHGLEILLLSFpdgareHGKYAEATKLTGDPNVPAGEVTFEAH--------DG 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 337 ESLCNFNELSRIVLeaqeqvrqeqqrqedrtedgegsgpptevrepenegaaplkeepvqfvlpVGVSSRNENYPRTCRm 416
Cdd:pfam12014  69 EIFREFNEGSRVVQ--------------------------------------------------VGVRSRDQNYPRTCR- 97

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1886460095 417 cFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFIWLELKSFSLYSRV 463
Cdd:pfam12014  98 -FYGVGHIAGHGFRNPRRIPGVLILFDEDHLGFIWLELKHFSLFSRV 143
F-box_SF super family cl45894
F-box domain superfamily; This short domain is commonly found at the N-terminus of various ...
 79-116 2.89e-16

F-box domain superfamily; This short domain is commonly found at the N-terminus of various proteins, and typically co-occurs with one or more other conserved domains or motifs, such as leucine rich repeats, WD40 repeats, kelch, tub, spry, and others. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression. One of the best researched roles of F-box proteins is their participation in SCF (Skp1-Cul1-F-box protein), a multi-protein complex that functions as a ubiquitin E3 ligase, where the role of the F-box protein is to recruit target substrates. Gene families containing the F-box are found greatly expanded in narrow taxonomic lineages, such as flowering plants and nematodes. In this hierarchical classification, many of the subfamilies are named according to their domain architectures.


The actual alignment was detected with superfamily member cd22102:

Pssm-ID: 459239  Cd Length: 48  Bit Score: 72.46  E-value: 2.89e-16
                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 1886460095  79 VEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRCRE 116
Cdd:cd22102    10 VEIFSSLPGTDLPSLAQVCKKFREILNTDTIWQRRCKE 47
 
Name Accession Description Interval E-value
Cyclin_D1_bind pfam12014
Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with ...
 265-463 4.06e-67

Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with normal and tumour cell proliferation and survival. The best characterized member of this family is the SCF FBXO31 (Skp1-Cul1-Rbx1-FBXO31) ubiquitin ligase complex mediates genotoxic stress-induced cyclin D1 degradation. FBXO31 possesses a unique substrate-binding beta barrel domain, whereas cyclin D1 binds to FBXO31 by tucking its free C-terminal carboxylate tail into an open cavity of the C-terminal FBXO31 beta-barrel. Biophysical and functional studies demonstrated that SCFFBXO31 is capable of recruiting and ubiquitinating cyclin D1 in a phosphorylation-independent manner.


Pssm-ID: 463431  Cd Length: 147  Bit Score: 212.08  E-value: 4.06e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 265 YRRIYLPPShpddLIRP--GLFKGTYGSHGLEIVMLSF------HGKHARVTKITGDPNIPAGQQTVEIDlmhriqlpNI 336
Cdd:pfam12014   1 YRRLYLPPT----LIRPfrGLFVGDYGGHGLEILLLSFpdgareHGKYAEATKLTGDPNVPAGEVTFEAH--------DG 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 337 ESLCNFNELSRIVLeaqeqvrqeqqrqedrtedgegsgpptevrepenegaaplkeepvqfvlpVGVSSRNENYPRTCRm 416
Cdd:pfam12014  69 EIFREFNEGSRVVQ--------------------------------------------------VGVRSRDQNYPRTCR- 97

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1886460095 417 cFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFIWLELKSFSLYSRV 463
Cdd:pfam12014  98 -FYGVGHIAGHGFRNPRRIPGVLILFDEDHLGFIWLELKHFSLFSRV 143
F-box_FBXO31 cd22102
F-box domain found in F-box only protein 31 (FBXO31) and similar proteins; FBXO31, also called ...
 79-116 2.89e-16

F-box domain found in F-box only protein 31 (FBXO31) and similar proteins; FBXO31, also called FBX31, or FBXO14, is a component of an SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. It specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. FBXO31 may act as a tumor suppressor. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438874  Cd Length: 48  Bit Score: 72.46  E-value: 2.89e-16
                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 1886460095  79 VEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRCRE 116
Cdd:cd22102    10 VEIFSSLPGTDLPSLAQVCKKFREILNTDTIWQRRCKE 47
F-box-like pfam12937
F-box-like; This is an F-box-like family.
 78-114 6.86e-06

F-box-like; This is an F-box-like family.


Pssm-ID: 463757 [Multi-domain]  Cd Length: 45  Bit Score: 42.86  E-value: 6.86e-06
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1886460095  78 LVEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRC 114
Cdd:pfam12937   9 LLQIFSYLDPKDLLRLALVCRRWRELASDDSLWRRLC 45
FBOX smart00256
A Receptor for Ubiquitination Targets;
79-113 3.05e-03

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 35.49  E-value: 3.05e-03
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 1886460095   79 VEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRR 113
Cdd:smart00256   7 EEILSKLDPKDLLRLRKVSRKWRSLIDSHDFWFKL 41
 
Name Accession Description Interval E-value
Cyclin_D1_bind pfam12014
Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with ...
 265-463 4.06e-67

Cyclin D1 binding domain; Ubiquitin-dependent proteolysis of cyclin D1 is associated with normal and tumour cell proliferation and survival. The best characterized member of this family is the SCF FBXO31 (Skp1-Cul1-Rbx1-FBXO31) ubiquitin ligase complex mediates genotoxic stress-induced cyclin D1 degradation. FBXO31 possesses a unique substrate-binding beta barrel domain, whereas cyclin D1 binds to FBXO31 by tucking its free C-terminal carboxylate tail into an open cavity of the C-terminal FBXO31 beta-barrel. Biophysical and functional studies demonstrated that SCFFBXO31 is capable of recruiting and ubiquitinating cyclin D1 in a phosphorylation-independent manner.


Pssm-ID: 463431  Cd Length: 147  Bit Score: 212.08  E-value: 4.06e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 265 YRRIYLPPShpddLIRP--GLFKGTYGSHGLEIVMLSF------HGKHARVTKITGDPNIPAGQQTVEIDlmhriqlpNI 336
Cdd:pfam12014   1 YRRLYLPPT----LIRPfrGLFVGDYGGHGLEILLLSFpdgareHGKYAEATKLTGDPNVPAGEVTFEAH--------DG 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1886460095 337 ESLCNFNELSRIVLeaqeqvrqeqqrqedrtedgegsgpptevrepenegaaplkeepvqfvlpVGVSSRNENYPRTCRm 416
Cdd:pfam12014  69 EIFREFNEGSRVVQ--------------------------------------------------VGVRSRDQNYPRTCR- 97

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1886460095 417 cFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFIWLELKSFSLYSRV 463
Cdd:pfam12014  98 -FYGVGHIAGHGFRNPRRIPGVLILFDEDHLGFIWLELKHFSLFSRV 143
F-box_FBXO31 cd22102
F-box domain found in F-box only protein 31 (FBXO31) and similar proteins; FBXO31, also called ...
 79-116 2.89e-16

F-box domain found in F-box only protein 31 (FBXO31) and similar proteins; FBXO31, also called FBX31, or FBXO14, is a component of an SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. It specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. FBXO31 may act as a tumor suppressor. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438874  Cd Length: 48  Bit Score: 72.46  E-value: 2.89e-16
                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 1886460095  79 VEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRCRE 116
Cdd:cd22102    10 VEIFSSLPGTDLPSLAQVCKKFREILNTDTIWQRRCKE 47
F-box-like pfam12937
F-box-like; This is an F-box-like family.
 78-114 6.86e-06

F-box-like; This is an F-box-like family.


Pssm-ID: 463757 [Multi-domain]  Cd Length: 45  Bit Score: 42.86  E-value: 6.86e-06
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 1886460095  78 LVEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRC 114
Cdd:pfam12937   9 LLQIFSYLDPKDLLRLALVCRRWRELASDDSLWRRLC 45
F-box_unchar cd22139
F-box domain found in uncharacterized F-box protein group similar to F-box only protein 3 ...
 81-114 2.20e-05

F-box domain found in uncharacterized F-box protein group similar to F-box only protein 3 (FBXO3); This subfamily corresponds to a group of uncharacterized F-box proteins which show sequence similarity to F-box only protein 3 (FBXO3). FBXO3, also called FBX3, is the substrate-recognition component of an SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, that mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. It also promotes ubiquitylation and transcriptional activity of AIRE (autoimmune regulator). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438911  Cd Length: 45  Bit Score: 41.46  E-value: 2.20e-05
                          10        20        30
                  ....*....|....*....|....*....|....
gi 1886460095  81 IFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRC 114
Cdd:cd22139    12 IFSFLSPKDLCQVALVCRRFNRLASDESLWKQIC 45
F-box_FBXO24 cd22098
F-box domain found in F-box only protein 24 (FBXO24) and similar proteins; FBXO24, also called ...
 80-115 1.04e-04

F-box domain found in F-box only protein 24 (FBXO24) and similar proteins; FBXO24, also called FBX24, is the substrate-recognition component of an SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. It recognizes deacetylated nucleoside diphosphate kinase A (NDPK-A) to enhance its degradation. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438870  Cd Length: 47  Bit Score: 39.61  E-value: 1.04e-04
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 1886460095  80 EIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRRCR 115
Cdd:cd22098    12 RIISFLPVKDIVSLGQTCRYFHEVCNSEAVWRRLCR 47
FBOX smart00256
A Receptor for Ubiquitination Targets;
79-113 3.05e-03

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 35.49  E-value: 3.05e-03
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 1886460095   79 VEIFASLPGTDLPSLAQVCTKFCRILHNDTIWRRR 113
Cdd:smart00256   7 EEILSKLDPKDLLRLRKVSRKWRSLIDSHDFWFKL 41
F-box_FBXL1 cd22114
F-box domain found in F-box/LRR-repeat protein 1 (FBXL1) and similar proteins; FBXL1, also ...
 78-110 5.95e-03

F-box domain found in F-box/LRR-repeat protein 1 (FBXL1) and similar proteins; FBXL1, also called S-phase kinase-associated protein 2, cyclin-A/CDK2-associated protein p45, F-box protein Skp2, or p45skp2, is the substrate-recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438886  Cd Length: 41  Bit Score: 34.69  E-value: 5.95e-03
                          10        20        30
                  ....*....|....*....|....*....|...
gi 1886460095  78 LVEIFASLPGTDLPSLAQVCTKFCRILHNDTIW 110
Cdd:cd22114     9 LLGIFSCLCLPDLLKVSQVCKRWYRLASDESLW 41
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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