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Conserved domains on  [gi|377947701|gb|EHV11366|]
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transposase domain protein, partial [Escherichia coli DEC4C]

Protein Classification

transposase( domain architecture ID 1000502)

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DDE_3 super family cl46863
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 20-86 6.35e-34

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


The actual alignment was detected with superfamily member PHA02517:

Pssm-ID: 481203 [Multi-domain]  Cd Length: 277  Bit Score: 116.89  E-value: 6.35e-34
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 377947701  20 LHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTV 86
Cdd:PHA02517   1 LGIAPSTYYRCQQQRHHPDKRRARAQHDDWLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTV 67
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 20-86 6.35e-34

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 116.89  E-value: 6.35e-34
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 377947701  20 LHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTV 86
Cdd:PHA02517   1 LGIAPSTYYRCQQQRHHPDKRRARAQHDDWLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTV 67
HTH_21 pfam13276
HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding ...
 45-86 6.57e-07

HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding function.


Pssm-ID: 463824 [Multi-domain]  Cd Length: 60  Bit Score: 42.17  E-value: 6.57e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 377947701  45 QHDDWLKREIQRVYDENHQVYGVRKVWRQLLREG-IRVARCTV 86
Cdd:pfam13276  1 ELEDELLEAIREIFEESRGTYGYRRITAELRREGgIRVNRKRV 43
 
Name Accession Description Interval E-value
PHA02517 PHA02517
putative transposase OrfB; Reviewed
 20-86 6.35e-34

putative transposase OrfB; Reviewed


Pssm-ID: 222853 [Multi-domain]  Cd Length: 277  Bit Score: 116.89  E-value: 6.35e-34
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 377947701  20 LHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTV 86
Cdd:PHA02517   1 LGIAPSTYYRCQQQRHHPDKRRARAQHDDWLKSEILRVYDENHQVYGVRKVWRQLNREGIRVARCTV 67
HTH_21 pfam13276
HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding ...
 45-86 6.57e-07

HTH-like domain; This domain contains a predicted helix-turn-helix suggesting a DNA-binding function.


Pssm-ID: 463824 [Multi-domain]  Cd Length: 60  Bit Score: 42.17  E-value: 6.57e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 377947701  45 QHDDWLKREIQRVYDENHQVYGVRKVWRQLLREG-IRVARCTV 86
Cdd:pfam13276  1 ELEDELLEAIREIFEESRGTYGYRRITAELRREGgIRVNRKRV 43
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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