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Conserved domains on  [gi|348667916|gb|EGZ07741|]
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hypothetical protein PHYSODRAFT_528012 [Phytophthora sojae]

Protein Classification

transposase family protein( domain architecture ID 229435)

transposase family protein might bind to the end of a transposon and catalyze the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0004803
PubMed:  20615441

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DDE_Tnp_4 super family cl48181
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 157-276 5.87e-17

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


The actual alignment was detected with superfamily member pfam13359:

Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 76.16  E-value: 5.87e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 348667916  157 VDGTVRGICRPGGAREQQCVYSGHKRKHALKFQTVATPDGMIFHLHGPAEGRRHDILLLRESGLEERVRRDGrFQDYFVY 236
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNLAF-GPGDYIL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 348667916  237 GDQAYGRTDVFVSPFK---GSSLTPAQAAINASMSKVRTSVEW 276
Cdd:pfam13359  80 ADKGFPISDWLLVPYKkpgGAQLSPEEVEFNRRIASARIHVER 122
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 157-276 5.87e-17

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 76.16  E-value: 5.87e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 348667916  157 VDGTVRGICRPGGAREQQCVYSGHKRKHALKFQTVATPDGMIFHLHGPAEGRRHDILLLRESGLEERVRRDGrFQDYFVY 236
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNLAF-GPGDYIL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 348667916  237 GDQAYGRTDVFVSPFK---GSSLTPAQAAINASMSKVRTSVEW 276
Cdd:pfam13359  80 ADKGFPISDWLLVPYKkpgGAQLSPEEVEFNRRIASARIHVER 122
transpos_IS5_4 NF033581
IS5 family transposase;
177-281 4.27e-08

IS5 family transposase;


Pssm-ID: 468098 [Multi-domain]  Cd Length: 284  Bit Score: 53.09  E-value: 4.27e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 348667916 177 YSGHKRKHALKFQTVATPD-GMIFHLHGPAeGRRHDILLLRESGL--EERVRRDGRFQ-DYFVYGDQAYGRTD-VFVSPF 251
Cdd:NF033581 145 KKGKKSRHGYKAHIGVDPKsGLVHSLVTTP-ANVHDSRAAKELLHgdEKTVYADKGYDgDEGYQGLREAGVKPhIPRKKS 223

                         90       100       110
                 ....*....|....*....|....*....|
gi 348667916 252 KGSSLTPAQAAINASMSKVRTSVEWPYGQV 281
Cdd:NF033581 224 RNRPLTEAQKRRNRLKSKIRARVEHVFGVL 253
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 157-276 5.87e-17

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 76.16  E-value: 5.87e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 348667916  157 VDGTVRGICRPGGAREQQCVYSGHKRKHALKFQTVATPDGMIFHLHGPAEGRRHDILLLRESGLEERVRRDGrFQDYFVY 236
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNLAF-GPGDYIL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 348667916  237 GDQAYGRTDVFVSPFK---GSSLTPAQAAINASMSKVRTSVEW 276
Cdd:pfam13359  80 ADKGFPISDWLLVPYKkpgGAQLSPEEVEFNRRIASARIHVER 122
transpos_IS5_4 NF033581
IS5 family transposase;
177-281 4.27e-08

IS5 family transposase;


Pssm-ID: 468098 [Multi-domain]  Cd Length: 284  Bit Score: 53.09  E-value: 4.27e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 348667916 177 YSGHKRKHALKFQTVATPD-GMIFHLHGPAeGRRHDILLLRESGL--EERVRRDGRFQ-DYFVYGDQAYGRTD-VFVSPF 251
Cdd:NF033581 145 KKGKKSRHGYKAHIGVDPKsGLVHSLVTTP-ANVHDSRAAKELLHgdEKTVYADKGYDgDEGYQGLREAGVKPhIPRKKS 223

                         90       100       110
                 ....*....|....*....|....*....|
gi 348667916 252 KGSSLTPAQAAINASMSKVRTSVEWPYGQV 281
Cdd:NF033581 224 RNRPLTEAQKRRNRLKSKIRARVEHVFGVL 253
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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