NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|288869468|gb|EFD01767|]
View 

PTS system, Lactose/Cellobiose specific IIB subunit [Hungatella hathewayi DSM 13479]

Protein Classification

PTS sugar transporter subunit IIB( domain architecture ID 10144917)

subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS)

CATH:  3.40.50.2300
EC:  2.7.1.-
Gene Ontology:  GO:0008643|GO:0009401|GO:0008982
PubMed:  8246840|2197982|11361063

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 2-89 4.34e-24

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


:

Pssm-ID: 99908  Cd Length: 89  Bit Score: 86.82  E-value: 4.34e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  2 KRIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIFVSITPTFDlsNVRIPTFSGIPFLTGI 81
Cdd:cd05566   1 KKILVACGTGVATSTVVASKVKELLKENGI--DVKVEQCKIAEVPSLLDDADLIVSTTKVPE--DYGIPVINGLPFLTGI 76


                ....*...
gi 288869468 82 GEEEIMDQ 89
Cdd:cd05566  77 GEDKVYEE 84
 
Name Accession Description Interval E-value
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 2-89 4.34e-24

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 86.82  E-value: 4.34e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  2 KRIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIFVSITPTFDlsNVRIPTFSGIPFLTGI 81
Cdd:cd05566   1 KKILVACGTGVATSTVVASKVKELLKENGI--DVKVEQCKIAEVPSLLDDADLIVSTTKVPE--DYGIPVINGLPFLTGI 76


                ....*...
gi 288869468 82 GEEEIMDQ 89
Cdd:cd05566  77 GEDKVYEE 84
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 1-89 1.47e-21

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 80.60  E-value: 1.47e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  1 MKRIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIFVSITPTfdLSNVRIPTFSGIPFLTG 80
Cdd:COG3414   1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGI--DAEVEHCDVSSAKSKADDADLIVTTTDL--AEELGIPVINVIPLLNG 76


                ....*....
gi 288869468 81 IGEEEIMDQ 89
Cdd:COG3414  77 IDKDEIKEK 85
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 3-89 8.78e-09

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 48.10  E-value: 8.78e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468   3 RIVVACGSGIATSGLVANKINNLLEERGMVGKARADSIDIKSIDLEIGTADIFVsITPTFDLSNV------RIPTFSGIP 76
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGIVEAQGAAGVNELTAEDIADDADVVI-LAPDVAVEDLarfagkPVYVIPVKD 79

                         90
                 ....*....|...
gi 288869468  77 FLTGIGEEEIMDQ 89
Cdd:pfam02302 80 ALGMKDAEEVLEK 92
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
1-94 6.41e-06

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 40.62  E-value: 6.41e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  1 MKR-IVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIfVSITPTFDLSNVRIPTFSGIPFLT 79
Cdd:PRK10310  1 MKRkIIVACGGAVATSTMAAEEIKELCQSHNI--PVELIQCRVNEIETYMDGVHL-ICTTARVDRSFGDIPLVHGMPFVS 77

                        90
                ....*....|....*
gi 288869468 80 GIGEEEIMDQFVGLL 94
Cdd:PRK10310 78 GVGIEALQNKILTIL 92
 
Name Accession Description Interval E-value
PTS_IIB_galactitol cd05566
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific ...
 2-89 4.34e-24

PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99908  Cd Length: 89  Bit Score: 86.82  E-value: 4.34e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  2 KRIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIFVSITPTFDlsNVRIPTFSGIPFLTGI 81
Cdd:cd05566   1 KKILVACGTGVATSTVVASKVKELLKENGI--DVKVEQCKIAEVPSLLDDADLIVSTTKVPE--DYGIPVINGLPFLTGI 76


                ....*...
gi 288869468 82 GEEEIMDQ 89
Cdd:cd05566  77 GEDKVYEE 84
SgaB COG3414
Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and ...
 1-89 1.47e-21

Phosphotransferase system, galactitol-specific IIB component [Carbohydrate transport and metabolism];


Pssm-ID: 442640  Cd Length: 94  Bit Score: 80.60  E-value: 1.47e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  1 MKRIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIFVSITPTfdLSNVRIPTFSGIPFLTG 80
Cdd:COG3414   1 MKKILVVCGSGLGTSTMLKMKIEKVLKELGI--DAEVEHCDVSSAKSKADDADLIVTTTDL--AEELGIPVINVIPLLNG 76


                ....*....
gi 288869468 81 IGEEEIMDQ 89
Cdd:COG3414  77 IDKDEIKEK 85
PTS_IIB cd00133
PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the ...
 3-89 2.99e-10

PTS_IIB: subunit IIB of enzyme II (EII) is the central energy-coupling domain of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In the multienzyme PTS complex, EII is a carbohydrate-specific permease consisting of two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system. The PTS is found only in bacteria, where it catalyzes the transport and phosphorylation of numerous monosaccharides, disaccharides, polyols, amino sugars, and other sugar derivatives. The four proteins (domains) forming the PTS phosphorylation cascade (EI, HPr, EIIA, and EIIB), can phosphorylate or interact with numerous non-PTS proteins thereby regulating their activity.


Pssm-ID: 99904  Cd Length: 84  Bit Score: 51.49  E-value: 2.99e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  3 RIVVACGSGIATSGLVANKINNLLEERGMVGKARAdsIDIKSIDLEIGtADIFVSiTPTFDLSNVRIPTFSGIPFLTGIG 82
Cdd:cd00133   1 KILVVCGSGIGSSSMLAEKLEKAAKELGIEVKVEA--QGLSEVIDLAD-ADLIIS-TVPLAARFLGKPVIVVSPLLNEKD 76


                ....*..
gi 288869468 83 EEEIMDQ 89
Cdd:cd00133  77 GEKILEK 83
PTS_IIB pfam02302
PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar ...
 3-89 8.78e-09

PTS system, Lactose/Cellobiose specific IIB subunit; The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The lactose/cellobiose-specific family are one of four structurally and functionally distinct group IIB PTS system cytoplasmic enzymes. The fold of IIB cellobiose shows similar structure to mammalian tyrosine phosphatases. This family also contains the fructose specific IIB subunit.


Pssm-ID: 396744  Cd Length: 92  Bit Score: 48.10  E-value: 8.78e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468   3 RIVVACGSGIATSGLVANKINNLLEERGMVGKARADSIDIKSIDLEIGTADIFVsITPTFDLSNV------RIPTFSGIP 76
Cdd:pfam02302  1 KILTACGAGMATSLMAAEALEKAAKELGIVEAQGAAGVNELTAEDIADDADVVI-LAPDVAVEDLarfagkPVYVIPVKD 79

                         90
                 ....*....|...
gi 288869468  77 FLTGIGEEEIMDQ 89
Cdd:pfam02302 80 ALGMKDAEEVLEK 92
PTS_IIB_bgl_like cd05568
PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory ...
 2-86 4.92e-06

PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory systems composed of a membrane-bound sugar-sensor (similar to BglF) and a transcription antiterminator (similar to BglG) which regulate expression of genes involved in sugar utilization. The domain architecture of the IIB-containing protein includes a region N-terminal to the IIB domain which is homologous to the BglG transcription antiterminator with an RNA-binding domain followed by two homologous domains, PRD1 and PRD2 (PTS Regulation Domains). C-terminal to the IIB domain is a domain similar to the PTS IIA domain. In this system, the BglG-like region and the IIB and IIA-like domains are all expressed together as a single multidomain protein. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include this sensory system with similarity to the bacterial bgl system, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and fructose systems.


Pssm-ID: 99910  Cd Length: 85  Bit Score: 40.95  E-value: 4.92e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  2 KRIVVACGSGIATSGLVANKINNLLEER---GMVGKARADSIDIKSIDLEIGTADIFVSITPTFDLSnvriptfsgiPFL 78
Cdd:cd05568   1 KKALVVCPSGIGTSRLLKSKLKKLFPEIeiiDVISLRELEEVDLDDYDLIISTVPLEDTDKPVIVVS----------PIL 70


                ....*...
gi 288869468 79 TgigEEEI 86
Cdd:cd05568  71 T---EEDI 75
PRK10310 PRK10310
PTS galactitol transporter subunit IIB;
1-94 6.41e-06

PTS galactitol transporter subunit IIB;


Pssm-ID: 182372  Cd Length: 94  Bit Score: 40.62  E-value: 6.41e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 288869468  1 MKR-IVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEIGTADIfVSITPTFDLSNVRIPTFSGIPFLT 79
Cdd:PRK10310  1 MKRkIIVACGGAVATSTMAAEEIKELCQSHNI--PVELIQCRVNEIETYMDGVHL-ICTTARVDRSFGDIPLVHGMPFVS 77

                        90
                ....*....|....*
gi 288869468 80 GIGEEEIMDQFVGLL 94
Cdd:PRK10310 78 GVGIEALQNKILTIL 92
BglG COG3711
Transcriptional antiterminator [Transcription];
2-54 7.34e-04

Transcriptional antiterminator [Transcription];


Pssm-ID: 442925 [Multi-domain]  Cd Length: 618  Bit Score: 36.76  E-value: 7.34e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 288869468   2 KRIVVACGSGIATSGLVANKINNLLEE---RGMVGKARADSIDIKSIDLEIGTADI 54
Cdd:COG3711  392 KRVLVVCSSGIGTSRLLKSRLKKLFPEieiIDVISYRELEEIDLEDYDLIISTVPL 447
PTS_IIB_ascorbate cd05563
PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific ...
 3-56 2.90e-03

PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.


Pssm-ID: 99905  Cd Length: 86  Bit Score: 33.65  E-value: 2.90e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....
gi 288869468  3 RIVVACGSGIATSGLVANKINNLLEERGMvgKARADSIDIKSIDLEigTADIFV 56
Cdd:cd05563   1 KILAVCGSGLGSSLMLKMNVEKVLKELGI--EAEVEHTDLGSAKAS--SADIIV 50
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH