NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|34483098|emb|CAE10097|]
View 

hypothetical protein WS0994 [Wolinella succinogenes]

Protein Classification

MoaD/ThiS family protein( domain architecture ID 10495630)

MoaD/ThiS family protein is a ubiquitin-like protein, may be involved in sulfur transfer

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
ThiS pfam02597
ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in ...
 20-80 6.80e-11

ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalyzed by IscS. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.


:

Pssm-ID: 396932 [Multi-domain]  Cd Length: 74  Bit Score: 53.06  E-value: 6.80e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 34483098   20 IGYLGELYECK-------EGITVKELVAQLGFRDEEVEG---AFVNYMITPK---NFTLKEGDRVALVPPGTPG 80
Cdd:pfam02597  1 VLLNAELRELAgkdeelaEGATVAELLEALGLRYPELRErvaVAVNGEIVPRlwlDTPLKDGDEVAIIPPVGGG 74
 
Name Accession Description Interval E-value
ThiS pfam02597
ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in ...
 20-80 6.80e-11

ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalyzed by IscS. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.


Pssm-ID: 396932 [Multi-domain]  Cd Length: 74  Bit Score: 53.06  E-value: 6.80e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 34483098   20 IGYLGELYECK-------EGITVKELVAQLGFRDEEVEG---AFVNYMITPK---NFTLKEGDRVALVPPGTPG 80
Cdd:pfam02597  1 VLLNAELRELAgkdeelaEGATVAELLEALGLRYPELRErvaVAVNGEIVPRlwlDTPLKDGDEVAIIPPVGGG 74
MoaD COG1977
Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; ...
 2-80 1.48e-08

Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; Molybdopterin synthase sulfur carrier subunit MoaD is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 441580 [Multi-domain]  Cd Length: 82  Bit Score: 47.51  E-value: 1.48e-08
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 34483098  2 KLSLKAFSTLRPHLKEQNIgylgELyECKEGITVKELVAQLGFRDEEVEGA--------FVNYMITPKNFTLKEGDRVAL 73
Cdd:COG1977  1 KVTVRYFAALREAAGKSEE----EV-ELPEGATVGDLLEALAARYPGLAERllrrrvrvAVNGEDVRLDTPLKDGDEVAL 75


               ....*..
gi 34483098 74 VPPGTPG 80
Cdd:COG1977 76 FPPVAGG 82
thiS TIGR01683
thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like ...
 24-74 5.59e-06

thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like thiamine biosynthesis protein related to MoaD, a molybdenum cofactor biosynthesis protein. Both proteins are involved in sulfur transfer. ThiS has a conserved Gly-Gly C-terminus that is modified, in reactions requiring ThiI, ThiF, IscS, and a sulfur atom from Cys, into the thiocarboxylate that provides the sulfur for thiazole biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Thiamine]


Pssm-ID: 273754 [Multi-domain]  Cd Length: 64  Bit Score: 40.24  E-value: 5.59e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 34483098   24 GELYECKEGITVKELVAQLGFRDEEVEGAfVNYMITPK----NFTLKEGDRVALV 74
Cdd:TIGR01683  5 GEPVEVEDGTTLAALLESLGLDPRRVAVA-VNGEIVPRsewdDTILKEGDRIEIV 58
Ubl_MoaD cd00754
ubiquitin-like (Ubl) domain found in molybdenum cofactor biosynthesis protein D (MoaD) and ...
 28-76 9.68e-04

ubiquitin-like (Ubl) domain found in molybdenum cofactor biosynthesis protein D (MoaD) and similar proteins; MoaD, also termed molybdopterin synthase sulfur carrier subunit, or MPT synthase subunit 1, or MPT synthase small subunit, or molybdopterin-converting factor small subunit, or molybdopterin-converting factor subunit 1, is a conserved small sulfur carrier protein that has beta-grasp ubiquitin-like (Ubl) fold involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor of a diverse group of redox enzymes. MoaD is activated in an ATP-dependent manner by sulfurtransferases similar to the activation mechanism of ubiquitin-activating enzyme E1.


Pssm-ID: 340452  Cd Length: 79  Bit Score: 34.86  E-value: 9.68e-04
                       10        20        30        40        50
               ....*....|....*....|....*....|....*....|....*....|....*
gi 34483098 28 ECKEGITVKELVAQLGFRDEEVEGAF------VNYMITPKNFTLKEGDRVALVPP 76
Cdd:cd00754 21 ELPAGTTVEELLEELAAKYPALAAILksvrvaVNQEYVDDDTELKDGDEVAFIPP 75
 
Name Accession Description Interval E-value
ThiS pfam02597
ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in ...
 20-80 6.80e-11

ThiS family; ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalyzed by IscS. MoaD, a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.


Pssm-ID: 396932 [Multi-domain]  Cd Length: 74  Bit Score: 53.06  E-value: 6.80e-11
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 34483098   20 IGYLGELYECK-------EGITVKELVAQLGFRDEEVEG---AFVNYMITPK---NFTLKEGDRVALVPPGTPG 80
Cdd:pfam02597  1 VLLNAELRELAgkdeelaEGATVAELLEALGLRYPELRErvaVAVNGEIVPRlwlDTPLKDGDEVAIIPPVGGG 74
MoaD COG1977
Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; ...
 2-80 1.48e-08

Molybdopterin synthase sulfur carrier subunit MoaD [Coenzyme transport and metabolism]; Molybdopterin synthase sulfur carrier subunit MoaD is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 441580 [Multi-domain]  Cd Length: 82  Bit Score: 47.51  E-value: 1.48e-08
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 34483098  2 KLSLKAFSTLRPHLKEQNIgylgELyECKEGITVKELVAQLGFRDEEVEGA--------FVNYMITPKNFTLKEGDRVAL 73
Cdd:COG1977  1 KVTVRYFAALREAAGKSEE----EV-ELPEGATVGDLLEALAARYPGLAERllrrrvrvAVNGEDVRLDTPLKDGDEVAL 75


               ....*..
gi 34483098 74 VPPGTPG 80
Cdd:COG1977 76 FPPVAGG 82
ThiS COG2104
Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; ...
 24-76 2.80e-08

Sulfur carrier protein ThiS (thiamine biosynthesis) [Coenzyme transport and metabolism]; Sulfur carrier protein ThiS (thiamine biosynthesis) is part of the Pathway/BioSystem: Thiamine biosynthesis


Pssm-ID: 441707 [Multi-domain]  Cd Length: 66  Bit Score: 46.22  E-value: 2.80e-08
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 34483098 24 GELYECKEGITVKELVAQLGFRDE----EVEGAFVnymitPK----NFTLKEGDRVALVPP 76
Cdd:COG2104  7 GEPREVPEGTTLADLLEELGLDPKgvavAVNGEIV-----PRsqwaSTVLKEGDRVEIVTA 62
thiS TIGR01683
thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like ...
 24-74 5.59e-06

thiamine biosynthesis protein ThiS; This model represents ThiS, a small, ubiquitin-like thiamine biosynthesis protein related to MoaD, a molybdenum cofactor biosynthesis protein. Both proteins are involved in sulfur transfer. ThiS has a conserved Gly-Gly C-terminus that is modified, in reactions requiring ThiI, ThiF, IscS, and a sulfur atom from Cys, into the thiocarboxylate that provides the sulfur for thiazole biosynthesis. [Biosynthesis of cofactors, prosthetic groups, and carriers, Thiamine]


Pssm-ID: 273754 [Multi-domain]  Cd Length: 64  Bit Score: 40.24  E-value: 5.59e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 34483098   24 GELYECKEGITVKELVAQLGFRDEEVEGAfVNYMITPK----NFTLKEGDRVALV 74
Cdd:TIGR01683  5 GEPVEVEDGTTLAALLESLGLDPRRVAVA-VNGEIVPRsewdDTILKEGDRIEIV 58
Ubl_MoaD cd00754
ubiquitin-like (Ubl) domain found in molybdenum cofactor biosynthesis protein D (MoaD) and ...
 28-76 9.68e-04

ubiquitin-like (Ubl) domain found in molybdenum cofactor biosynthesis protein D (MoaD) and similar proteins; MoaD, also termed molybdopterin synthase sulfur carrier subunit, or MPT synthase subunit 1, or MPT synthase small subunit, or molybdopterin-converting factor small subunit, or molybdopterin-converting factor subunit 1, is a conserved small sulfur carrier protein that has beta-grasp ubiquitin-like (Ubl) fold involved in biosynthesis of the molybdenum cofactor (Moco), an essential cofactor of a diverse group of redox enzymes. MoaD is activated in an ATP-dependent manner by sulfurtransferases similar to the activation mechanism of ubiquitin-activating enzyme E1.


Pssm-ID: 340452  Cd Length: 79  Bit Score: 34.86  E-value: 9.68e-04
                       10        20        30        40        50
               ....*....|....*....|....*....|....*....|....*....|....*
gi 34483098 28 ECKEGITVKELVAQLGFRDEEVEGAF------VNYMITPKNFTLKEGDRVALVPP 76
Cdd:cd00754 21 ELPAGTTVEELLEELAAKYPALAAILksvrvaVNQEYVDDDTELKDGDEVAFIPP 75
ThiS-like pfam14453
ThiS-like ubiquitin; A member of the ubiquitin superfamily that is often fused to the ...
 24-77 8.92e-03

ThiS-like ubiquitin; A member of the ubiquitin superfamily that is often fused to the ThiF-like (E1)- ubiquitin activating enzyme and is present in gene neighborhoods with components of the thiamine biosynthesis pathway.


Pssm-ID: 464178  Cd Length: 57  Bit Score: 31.76  E-value: 8.92e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 34483098   24 GELYECKEGITVKELVAQLGFrDEEVegAFVNYMITPKNFTLKEGDRVALVPPG 77
Cdd:pfam14453  7 EKELDVNEGTTLYALKNQYKP-DADV--VILNGFPTKEDTLLKEGDEVFLIKKG 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH