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Conserved domains on  [gi|157889259|dbj|BAF81076|]
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2,4-dichlorophenol hydroxylase, partial [Burkholderia sp. M10-VN8-1W]

Protein Classification

Rossmann-fold NAD(P)-binding domain-containing protein( domain architecture ID 229380)

Rossmann-fold NAD(P)-binding domain-containing protein may function as an oxidoreductase

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NADB_Rossmann super family cl21454
Rossmann-fold NAD(P)(+)-binding proteins; A large family of proteins that share a ...
 1-160 6.62e-48

Rossmann-fold NAD(P)(+)-binding proteins; A large family of proteins that share a Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The NADB domain is found in numerous dehydrogenases of metabolic pathways such as glycolysis, and many other redox enzymes. NAD binding involves numerous hydrogen-bonds and van der Waals contacts, in particular H-bonding of residues in a turn between the first strand and the subsequent helix of the Rossmann-fold topology. Characteristically, this turn exhibits a consensus binding pattern similar to GXGXXG, in which the first 2 glycines participate in NAD(P)-binding, and the third facilitates close packing of the helix to the beta-strand. Typically, proteins in this family contain a second domain in addition to the NADB domain, which is responsible for specifically binding a substrate and catalyzing a particular enzymatic reaction.


The actual alignment was detected with superfamily member pfam01494:

Pssm-ID: 473865 [Multi-domain]  Cd Length: 348  Bit Score: 158.26  E-value: 6.62e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259    1 EILRDLGLEAEARLYAVPNDLMGENTICaslageefgrirtfgtdVRRRADYD-ECSPTSVCDLPQNYLEPILVKSAALD 79
Cdd:pfam01494  51 ELLRQAGLEDRILAEGVPHEGMGLAFYN-----------------TRRRADLDfLTSPPRVTVYPQTELEPILVEHAEAR 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   80 GCKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGK-SGSINLLFEA-DLDR 157
Cdd:pfam01494 114 GAQVRFGTEVLSLEQDGDGVTAVVRDRRDGEEYTVRAKYLVGCDGGRSPVRKTLGIEFEGFEGVpFGSLDVLFDApDLSD 193


                  ...
gi 157889259  158 YVA 160
Cdd:pfam01494 194 PVE 196
 
Name Accession Description Interval E-value
FAD_binding_3 pfam01494
FAD binding domain; This domain is involved in FAD binding in a number of enzymes.
 1-160 6.62e-48

FAD binding domain; This domain is involved in FAD binding in a number of enzymes.


Pssm-ID: 396193 [Multi-domain]  Cd Length: 348  Bit Score: 158.26  E-value: 6.62e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259    1 EILRDLGLEAEARLYAVPNDLMGENTICaslageefgrirtfgtdVRRRADYD-ECSPTSVCDLPQNYLEPILVKSAALD 79
Cdd:pfam01494  51 ELLRQAGLEDRILAEGVPHEGMGLAFYN-----------------TRRRADLDfLTSPPRVTVYPQTELEPILVEHAEAR 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   80 GCKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGK-SGSINLLFEA-DLDR 157
Cdd:pfam01494 114 GAQVRFGTEVLSLEQDGDGVTAVVRDRRDGEEYTVRAKYLVGCDGGRSPVRKTLGIEFEGFEGVpFGSLDVLFDApDLSD 193


                  ...
gi 157889259  158 YVA 160
Cdd:pfam01494 194 PVE 196
PRK06126 PRK06126
hypothetical protein; Provisional
 1-164 1.57e-26

hypothetical protein; Provisional


Pssm-ID: 235704 [Multi-domain]  Cd Length: 545  Bit Score: 104.30  E-value: 1.57e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   1 EILRDLGLEAEARLYAVPNDLMGENTICASLAGEEFGRIRTFGTDVRRRADYDECSPTSVCDL----PQNYLEPILVKSA 76
Cdd:PRK06126  57 EHFRRLGIADEVRSAGLPVDYPTDIAYFTRLTGYELARFRLPSAREAITPVGGPDGSWPSPELphriPQKYLEPILLEHA 136

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259  77 -ALDGCKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGKSGSINLLFEA-D 154
Cdd:PRK06126 137 aAQPGVTLRYGHRLTDFEQDADGVTATVEDLDGGESLTIRADYLVGCDGARSAVRRSLGISYEGTSGLQRDLSIYIRApG 216

                        170
                 ....*....|
gi 157889259 155 LDRYVAHRPS 164
Cdd:PRK06126 217 LAALVGHDPA 226
UbiH COG0654
2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme ...
 1-163 7.05e-18

2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 440419 [Multi-domain]  Cd Length: 326  Bit Score: 78.44  E-value: 7.05e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   1 EILRDLGLEAEARLYAVPNDLMgenTICASLAGEEFGRIrtfgtdvrrraDYDECSPTSVCDLPQNYLEPILVKSAALDG 80
Cdd:COG0654   53 ELLRRLGLWDRLLARGAPIRGI---RVRDGSDGRVLARF-----------DAAETGLPAGLVVPRADLERALLEAARALG 118

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259  81 CKVRFDTEYLGHEQDADGVSSRLRDrlnGEefTVRSKYLIGADGANSRVVSDLDLPLEGTMGKSGSINLLFEADLDRYVA 160
Cdd:COG0654  119 VELRFGTEVTGLEQDADGVTVTLAD---GR--TLRADLVVGADGARSAVRRLLGIGFTGRDYPQRALWAGVRTELRARLA 193


                 ...
gi 157889259 161 HRP 163
Cdd:COG0654  194 AAG 196
GG-red-SF TIGR02032
geranylgeranyl reductase family; This model represents a subfamily which includes ...
 38-138 1.40e-07

geranylgeranyl reductase family; This model represents a subfamily which includes geranylgeranyl reductases involved in chlorophyll and bacteriochlorophyll biosynthesis as well as other related enzymes which may also act on geranylgeranyl groups or related substrates. [Biosynthesis of cofactors, prosthetic groups, and carriers, Chlorophyll and bacteriochlorphyll]


Pssm-ID: 273936 [Multi-domain]  Cd Length: 295  Bit Score: 49.62  E-value: 1.40e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   38 RIRTFGTDVRRRADYDECsptsvcdlpqnylepiLVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRdrlnGEEFTVRSK 117
Cdd:TIGR02032  79 PIETELAYVIDRDAFDEQ----------------LAERAQEAGAELRLGTRVLDVEIHDDRVVVIVR----GSEGTVTAK 138

                          90       100
                  ....*....|....*....|.
gi 157889259  118 YLIGADGANSRVVSDLDLPLE 138
Cdd:TIGR02032 139 IVIGADGSRSIVAKKLGLKKE 159
 
Name Accession Description Interval E-value
FAD_binding_3 pfam01494
FAD binding domain; This domain is involved in FAD binding in a number of enzymes.
 1-160 6.62e-48

FAD binding domain; This domain is involved in FAD binding in a number of enzymes.


Pssm-ID: 396193 [Multi-domain]  Cd Length: 348  Bit Score: 158.26  E-value: 6.62e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259    1 EILRDLGLEAEARLYAVPNDLMGENTICaslageefgrirtfgtdVRRRADYD-ECSPTSVCDLPQNYLEPILVKSAALD 79
Cdd:pfam01494  51 ELLRQAGLEDRILAEGVPHEGMGLAFYN-----------------TRRRADLDfLTSPPRVTVYPQTELEPILVEHAEAR 113

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   80 GCKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGK-SGSINLLFEA-DLDR 157
Cdd:pfam01494 114 GAQVRFGTEVLSLEQDGDGVTAVVRDRRDGEEYTVRAKYLVGCDGGRSPVRKTLGIEFEGFEGVpFGSLDVLFDApDLSD 193


                  ...
gi 157889259  158 YVA 160
Cdd:pfam01494 194 PVE 196
PRK06126 PRK06126
hypothetical protein; Provisional
 1-164 1.57e-26

hypothetical protein; Provisional


Pssm-ID: 235704 [Multi-domain]  Cd Length: 545  Bit Score: 104.30  E-value: 1.57e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   1 EILRDLGLEAEARLYAVPNDLMGENTICASLAGEEFGRIRTFGTDVRRRADYDECSPTSVCDL----PQNYLEPILVKSA 76
Cdd:PRK06126  57 EHFRRLGIADEVRSAGLPVDYPTDIAYFTRLTGYELARFRLPSAREAITPVGGPDGSWPSPELphriPQKYLEPILLEHA 136

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259  77 -ALDGCKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGKSGSINLLFEA-D 154
Cdd:PRK06126 137 aAQPGVTLRYGHRLTDFEQDADGVTATVEDLDGGESLTIRADYLVGCDGARSAVRRSLGISYEGTSGLQRDLSIYIRApG 216

                        170
                 ....*....|
gi 157889259 155 LDRYVAHRPS 164
Cdd:PRK06126 217 LAALVGHDPA 226
UbiH COG0654
2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme ...
 1-163 7.05e-18

2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases [Coenzyme transport and metabolism, Energy production and conversion]; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 440419 [Multi-domain]  Cd Length: 326  Bit Score: 78.44  E-value: 7.05e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   1 EILRDLGLEAEARLYAVPNDLMgenTICASLAGEEFGRIrtfgtdvrrraDYDECSPTSVCDLPQNYLEPILVKSAALDG 80
Cdd:COG0654   53 ELLRRLGLWDRLLARGAPIRGI---RVRDGSDGRVLARF-----------DAAETGLPAGLVVPRADLERALLEAARALG 118

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259  81 CKVRFDTEYLGHEQDADGVSSRLRDrlnGEefTVRSKYLIGADGANSRVVSDLDLPLEGTMGKSGSINLLFEADLDRYVA 160
Cdd:COG0654  119 VELRFGTEVTGLEQDADGVTVTLAD---GR--TLRADLVVGADGARSAVRRLLGIGFTGRDYPQRALWAGVRTELRARLA 193


                 ...
gi 157889259 161 HRP 163
Cdd:COG0654  194 AAG 196
mhpA PRK06183
bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase;
76-140 9.52e-12

bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase;


Pssm-ID: 235727 [Multi-domain]  Cd Length: 500  Bit Score: 61.85  E-value: 9.52e-12
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 157889259  76 AALDGCKVRFDTEYLGHEQDADGVSSRLRDRlNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGT 140
Cdd:PRK06183 124 ARFPHVRVRFGHEVTALTQDDDGVTVTLTDA-DGQRETVRARYVVGCDGANSFVRRTLGVPFEDL 187
PRK06184 PRK06184
hypothetical protein; Provisional
 64-162 1.73e-11

hypothetical protein; Provisional


Pssm-ID: 235728 [Multi-domain]  Cd Length: 502  Bit Score: 61.15  E-value: 1.73e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259  64 PQNYLEPILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRDRLNGEefTVRSKYLIGADGANSRVVSDLDLPLEgtmGK 143
Cdd:PRK06184 107 PQWRTERILRERLAELGHRVEFGCELVGFEQDADGVTARVAGPAGEE--TVRARYLVGADGGRSFVRKALGIGFP---GE 181

                         90       100
                 ....*....|....*....|....
gi 157889259 144 SGSINLLFEAD-----LDRYVAHR 162
Cdd:PRK06184 182 TLGIDRMLVADvsltgLDRDAWHQ 205
GG-red-SF TIGR02032
geranylgeranyl reductase family; This model represents a subfamily which includes ...
 38-138 1.40e-07

geranylgeranyl reductase family; This model represents a subfamily which includes geranylgeranyl reductases involved in chlorophyll and bacteriochlorophyll biosynthesis as well as other related enzymes which may also act on geranylgeranyl groups or related substrates. [Biosynthesis of cofactors, prosthetic groups, and carriers, Chlorophyll and bacteriochlorphyll]


Pssm-ID: 273936 [Multi-domain]  Cd Length: 295  Bit Score: 49.62  E-value: 1.40e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   38 RIRTFGTDVRRRADYDECsptsvcdlpqnylepiLVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRdrlnGEEFTVRSK 117
Cdd:TIGR02032  79 PIETELAYVIDRDAFDEQ----------------LAERAQEAGAELRLGTRVLDVEIHDDRVVVIVR----GSEGTVTAK 138

                          90       100
                  ....*....|....*....|.
gi 157889259  118 YLIGADGANSRVVSDLDLPLE 138
Cdd:TIGR02032 139 IVIGADGSRSIVAKKLGLKKE 159
PRK08244 PRK08244
monooxygenase;
63-140 1.47e-07

monooxygenase;


Pssm-ID: 236199 [Multi-domain]  Cd Length: 493  Bit Score: 49.74  E-value: 1.47e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157889259  63 LPQNYLEPILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRDRlNGEEfTVRSKYLIGADGANSRVVSDLDLPLEGT 140
Cdd:PRK08244  97 LPQAETEKVLEEHARSLGVEIFRGAEVLAVRQDGDGVEVVVRGP-DGLR-TLTSSYVVGADGAGSIVRKQAGIAFPGT 172
PRK08132 PRK08132
FAD-dependent oxidoreductase; Provisional
 63-140 9.62e-07

FAD-dependent oxidoreductase; Provisional


Pssm-ID: 236158 [Multi-domain]  Cd Length: 547  Bit Score: 47.17  E-value: 9.62e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157889259  63 LPQNYLEPILVKSA-ALDGCKVRFDTEYLGHEQDADGVssRLRDRLNGEEFTVRSKYLIGADGANSRVVSDLDLPLEGT 140
Cdd:PRK08132 122 LQQYYVEGYLVERAqALPNIDLRWKNKVTGLEQHDDGV--TLTVETPDGPYTLEADWVIACDGARSPLREMLGLEFEGR 198
FixC COG0644
Dehydrogenase (flavoprotein) [Energy production and conversion];
68-135 7.02e-06

Dehydrogenase (flavoprotein) [Energy production and conversion];


Pssm-ID: 440409 [Multi-domain]  Cd Length: 281  Bit Score: 44.57  E-value: 7.02e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 157889259  68 LEPILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRDrlngeEFTVRSKYLIGADGANSRVVSDLDL 135
Cdd:COG0644   88 FDRWLAEQAEEAGAEVRTGTRVTDVLRDDGRVVVRTGD-----GEEIRADYVVDADGARSLLARKLGL 150
PRK07538 PRK07538
hypothetical protein; Provisional
 81-129 2.61e-05

hypothetical protein; Provisional


Pssm-ID: 236046 [Multi-domain]  Cd Length: 413  Bit Score: 42.96  E-value: 2.61e-05
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 157889259  81 CKVRFDTEYLGHEQDADGVSSRLRDRLNGEEFTVRSKYLIGADGANSRV 129
Cdd:PRK07538 119 DAVRTGHRVVGFEQDADVTVVFLGDRAGGDLVSVRGDVLIGADGIHSAV 167
PRK07190 PRK07190
FAD-binding protein;
63-138 1.31e-04

FAD-binding protein;


Pssm-ID: 235955 [Multi-domain]  Cd Length: 487  Bit Score: 40.95  E-value: 1.31e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 157889259  63 LPQNYLEPILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRdrlNGEefTVRSKYLIGADGANSRVVSDLDLPLE 138
Cdd:PRK07190 106 LGQSYVEKLLDDKLKEAGAAVKRNTSVVNIELNQAGCLTTLS---NGE--RIQSRYVIGADGSRSFVRNHFNVPFE 176
PRK08294 PRK08294
phenol 2-monooxygenase; Provisional
 85-129 2.42e-04

phenol 2-monooxygenase; Provisional


Pssm-ID: 236223 [Multi-domain]  Cd Length: 634  Bit Score: 40.36  E-value: 2.42e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 157889259  85 FDTEYLGHEQDADG---VSSRLR---DRLNGEEFTVRSKYLIGADGANSRV 129
Cdd:PRK08294 162 YGREFVDLEVDEEGeypVTVTLRrtdGEHEGEEETVRAKYVVGCDGARSRV 212
PRK11445 PRK11445
FAD-binding protein;
108-129 7.80e-04

FAD-binding protein;


Pssm-ID: 183139 [Multi-domain]  Cd Length: 351  Bit Score: 38.50  E-value: 7.80e-04
                         10        20
                 ....*....|....*....|..
gi 157889259 108 NGEEFTVRSKYLIGADGANSRV 129
Cdd:PRK11445 138 DGWEQHITARYLVGADGANSMV 159
PRK08163 PRK08163
3-hydroxybenzoate 6-monooxygenase;
6-129 1.24e-03

3-hydroxybenzoate 6-monooxygenase;


Pssm-ID: 181262 [Multi-domain]  Cd Length: 396  Bit Score: 38.09  E-value: 1.24e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 157889259   6 LGLEAEARLYAVPNDLMGENTicaSLAGEEFGRIRTfGTDVRRRADYdecsPTSV---CDLPQNYLEpilvksAALDGCK 82
Cdd:PRK08163  59 LGVGEAARQRAVFTDHLTMMD---AVDAEEVVRIPT-GQAFRARFGN----PYAVihrADIHLSLLE------AVLDHPL 124

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 157889259  83 VRFDT--EYLGHEQDADGVSSRLRDrlnGEEFTVRSkyLIGADGANSRV 129
Cdd:PRK08163 125 VEFRTstHVVGIEQDGDGVTVFDQQ---GNRWTGDA--LIGCDGVKSVV 168
PRK07045 PRK07045
putative monooxygenase; Reviewed
 71-138 1.54e-03

putative monooxygenase; Reviewed


Pssm-ID: 136171 [Multi-domain]  Cd Length: 388  Bit Score: 37.96  E-value: 1.54e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 157889259  71 ILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRdrLNGEEfTVRSKYLIGADGANSRVVSD-LDLPLE 138
Cdd:PRK07045 112 LLAKLDGLPNVRLRFETSIERIERDADGTVTSVT--LSDGE-RVAPTVLVGADGARSMIRDDvLRMPAE 177
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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