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Conserved domains on  [gi|1537797680|gb|AZN75416|]
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TENA/THI-4 family protein [Bifidobacterium pseudocatenulatum]

Protein Classification

TenA family protein( domain architecture ID 14443657)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 26-229 3.66e-66

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


:

Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 203.19  E-value: 3.66e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQG--IMAHAIELAPRQETKDMLAKQSQWFADNEA 103
Cdd:cd19358     2 SDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFlrLLGKAVAKAPDLEAKLRLARFLGFLANDEN 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAgvVQQIPAHKGWVD 182
Cdd:cd19358    82 DYFERAFAALGVSEADREAPPLLPATRAFIDLMLEAARsGSYAEILTVLLVAEWLYLDWASRAAAA--APLRFKHQEWID 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1537797680 183 LHEGDYFRAWTGRLIALVNEYASVDGSEADV---FRTIVHLERRFFEDSY 229
Cdd:cd19358   160 LHSGPEFEAWVDFLRDEVDRVGPTEEERERLeavFARAVELEIAFFDAAY 209
 
Name Accession Description Interval E-value
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 26-229 3.66e-66

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 203.19  E-value: 3.66e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQG--IMAHAIELAPRQETKDMLAKQSQWFADNEA 103
Cdd:cd19358     2 SDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFlrLLGKAVAKAPDLEAKLRLARFLGFLANDEN 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAgvVQQIPAHKGWVD 182
Cdd:cd19358    82 DYFERAFAALGVSEADREAPPLLPATRAFIDLMLEAARsGSYAEILTVLLVAEWLYLDWASRAAAA--APLRFKHQEWID 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1537797680 183 LHEGDYFRAWTGRLIALVNEYASVDGSEADV---FRTIVHLERRFFEDSY 229
Cdd:cd19358   160 LHSGPEFEAWVDFLRDEVDRVGPTEEERERLeavFARAVELEIAFFDAAY 209
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 23-229 7.74e-45

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 149.26  E-value: 7.74e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  23 GSVADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRQETKDMLAKQSQWFAD 100
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHfaRALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 101 NEATYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAGVVQQiPAHKG 179
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAAsGDYAELLAALLPCEWGYAEIGKRLAERPLPPD-HPYAE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1537797680 180 WVDLHEGDYFRAWTGRLIALVNEYASVDGSE-----ADVFRTIVHLERRFFEDSY 229
Cdd:COG0819   160 WIETYASEEFQELVEWLIALLDRLAATASEAererlEEAFRTASRLEYAFWDMAY 214
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 37-224 3.58e-14

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 68.92  E-value: 3.58e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  37 WNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIELA--PRQETKDMLAKQSQWFADNEATYFTGFLKEYG 114
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASksPDLEVRREWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 115 ITDEEYNNSEQTPANREYCEYLTKLV-QGTWEELITALCCMEWIYLAWAKRTIDAGVVQQIPAHKGWVDLHEGDYFRA-- 191
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFArRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEYFRAav 167

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1537797680 192 -WTGRLIALVNEYASVDGSEA--DVFRTIVHLERRF 224
Cdd:pfam03070 168 eEAKRLLDRLLAYVSDEQLEElqEIFLKALEFEYSF 203
 
Name Accession Description Interval E-value
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 26-229 3.66e-66

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 203.19  E-value: 3.66e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQG--IMAHAIELAPRQETKDMLAKQSQWFADNEA 103
Cdd:cd19358     2 SDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFlrLLGKAVAKAPDLEAKLRLARFLGFLANDEN 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAgvVQQIPAHKGWVD 182
Cdd:cd19358    82 DYFERAFAALGVSEADREAPPLLPATRAFIDLMLEAARsGSYAEILTVLLVAEWLYLDWASRAAAA--APLRFKHQEWID 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 1537797680 183 LHEGDYFRAWTGRLIALVNEYASVDGSEADV---FRTIVHLERRFFEDSY 229
Cdd:cd19358   160 LHSGPEFEAWVDFLRDEVDRVGPTEEERERLeavFARAVELEIAFFDAAY 209
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 23-229 7.74e-45

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 149.26  E-value: 7.74e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  23 GSVADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRQETKDMLAKQSQWFAD 100
Cdd:COG0819     1 MSFSERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHfaRALALAAAKAPDLEDMRFLAGLAAGLLE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 101 NEATYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAGVVQQiPAHKG 179
Cdd:COG0819    81 VELALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAAsGDYAELLAALLPCEWGYAEIGKRLAERPLPPD-HPYAE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1537797680 180 WVDLHEGDYFRAWTGRLIALVNEYASVDGSE-----ADVFRTIVHLERRFFEDSY 229
Cdd:COG0819   160 WIETYASEEFQELVEWLIALLDRLAATASEAererlEEAFRTASRLEYAFWDMAY 214
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 37-226 1.64e-25

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 98.58  E-value: 1.64e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  37 WNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHA--IELAPRQETKDMLAKQSQwFADNEATYFTGFLKEYG 114
Cdd:cd16099     2 WDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALAlaAAKAPDLELRTFLAELIN-VLDDELELHEKLLAELG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 115 ITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAGVVqqIPAHKGWVDLHEGDYFRAWT 193
Cdd:cd16099    81 ISEEDLSEAEPNPATLAYTNHLLRVAArGTPAEGLAALLPCYWSYGEIGRRLAASLPE--HPPYRFWIDFYASDEYQELV 158

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1537797680 194 GRLIALVNEYASVDGSE----ADVFRTIVHLERRFFE 226
Cdd:cd16099   159 EELLQLLDQLAAAGEEEkeelKEIFLTSLRYELMFWD 195
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 32-227 3.57e-18

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 79.67  E-value: 3.57e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  32 SAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGI--MAHAIELAPRQETkDMLAKQSQWFADN------EA 103
Cdd:cd19357     7 LHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIrfLGSLLARAPLPSS-SLNQRLLDVLLGAlanlrrEL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFLKEYGItDEEYNNSEQTPANREYCEYLTKLVQG--TWEELITALCCMEWIYL-AWAK-RTIDAGVVQQIPAHKG 179
Cdd:cd19357    86 AFFEETAAEYGL-DLPGLGVPPSPATRAYVDFLASLASEgvSYLEGLVVLWATEKVYLdAWSYaRSFLPSDADGGALYRE 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1537797680 180 WVDLHEGDYFRAWTGRLIALVNEYASVDGSEA-----DVFRTIVHLERRFFED 227
Cdd:cd19357   165 FIPNWTSPEFAAFVDRLGDLVDEALEQAGEEVleraeEVWRRVLELEEAFWPM 217
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 26-229 2.55e-17

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 77.21  E-value: 2.55e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIELAPRQETKDMlakqsQWFAD-NEAT 104
Cdd:cd19366     2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETM-----GRFAElLHGT 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 105 YFT------GFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITA-LCCMeWIYLAWAKRTIDAGVVQQIPA 176
Cdd:cd19366    77 LNTemdlhrQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQtGTLAELLAAlLPCA-WGYAEIGKRLAEQGGALEHNP 155

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1537797680 177 HKGWVDLHEGDYFRAWTGRLIALVNEYASvDGSEA------DVFRTIVHLERRFFEDSY 229
Cdd:cd19366   156 YREWIEMYSSDEFTELADWLIDLLDELAE-GKSEAelerleEIFLTSSRYEYMFWDMAY 213
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 25-229 3.91e-17

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 76.59  E-value: 3.91e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  25 VADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIeLAPRQETKDmLAKQSQWFADNEAT 104
Cdd:cd19363     1 ITGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSI-LASKAESLD-LMRELLELAYGEAT 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 105 Y----FTGFLKEYGITDEEYNNSEQTPANREYCEYL-TKLVQGTWEELITALCCMEWIYLAWAKRTIDAGVVQQIPAHKG 179
Cdd:cd19363    79 TefanYEELLDELGLSLEDAIKVEPFPTNVAYMNFLlSTSSLGSFYEGLAALLPCFWSYLEIAEYHKDKLSENPNDIYRD 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1537797680 180 WVDLHEGDYFRAWTGRLIALVNEYASVDGSEA--DVFRTIVHLERRFFEDSY 229
Cdd:cd19363   159 WASVYLSKEYKELVERLRRIVDKYGEGEPFEKlkRIFKTASKYEYMFWDAAY 210
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 37-224 3.58e-14

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 68.92  E-value: 3.58e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  37 WNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIELA--PRQETKDMLAKQSQWFADNEATYFTGFLKEYG 114
Cdd:pfam03070   8 WKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASksPDLEVRREWENRIVDHDGNEIELHLRLAEALG 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 115 ITDEEYNNSEQTPANREYCEYLTKLV-QGTWEELITALCCMEWIYLAWAKRTIDAGVVQQIPAHKGWVDLHEGDYFRA-- 191
Cdd:pfam03070  88 LSREDLSAYKPLPATRAYVRYLLDFArRGSWLEAVAALLPCLFVYQEIASRLGEKIRALEGPEYYEWVKTYASEYFRAav 167

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1537797680 192 -WTGRLIALVNEYASVDGSEA--DVFRTIVHLERRF 224
Cdd:pfam03070 168 eEAKRLLDRLLAYVSDEQLEElqEIFLKALEFEYSF 203
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 27-227 1.54e-13

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 66.77  E-value: 1.54e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  27 DQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRQETKDMLAKqsqwFADN--- 101
Cdd:cd19365     2 EALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDyaRALALLAARAPDPEEQVFFAR----FAAGaie 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 102 -EATYFTGFLKEYGITDEEynnsEQTPANREYCEYLTKLV-QGTWEELITA-LCCMeWIYLAWAKRTIDAGVvqqiPAH- 177
Cdd:cd19365    78 vERELHRSFLGEFGIDAAA----EPSPVTLAYTSFLLATAaTGPYAVAVAAvLPCF-WIYAEVGKRLAAAAS----PNHp 148

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1537797680 178 -KGWVDLHEGDYFRAWTGRLIALVNEYASVDGSE-----ADVFRTIVHLERRFFED 227
Cdd:cd19365   149 yQDWIDTYSDEEFAEAVRRAIAIVDRLAAEASPEerarmLEAFLRASRLEWMFWDA 204
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 27-229 6.90e-13

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 65.28  E-value: 6.90e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  27 DQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQD---FKFFNQgIMAHAIELAPRQETKDMLAKQSQWFADNEA 103
Cdd:cd19364     3 EELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDayyLKHFAK-LHALAAAKADDPAIKALLLEGAQGLAEGEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFLKEYGITDEEYNNSEQTPANREYCEYL-TKLVQGTWEELITALCCMEWIYLAWAKRTIDAGvvQQIPAHKGWVD 182
Cdd:cd19364    82 ALRETFFKELGITEEEIAQTPPAPTAYHYVSHMyRQLNEGSVAEAVAALLPCYWLYQEIGERLADAG--SPVPLYQRWID 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1537797680 183 LHEGDYFRAWTGRLIALVNEYASvDGSEA------DVFRTIVHLERRFFEDSY 229
Cdd:cd19364   160 TYASDEFTESVQQQIDLVDRLAE-EASEEerekmkQAFLISSYYELQFWEMAY 211
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 37-226 5.70e-12

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 62.63  E-value: 5.70e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  37 WNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRqeTKDMlakqsQWFAD-----NEATYFT-- 107
Cdd:cd19369     3 WEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEyaKVFAMGIYKSRT--MKEM-----QFFYSslsfvNEDETATri 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 108 GFLKEYGITDEEYNNSEQTPANREYCEY-LTKLVQGTWEELITA-LCCM---EWIYLAWAKRTIDagVVQQIPaHKGWVD 182
Cdd:cd19369    76 KYLKEFGLTDEDIEKIEPLPENKAYTDYmLGIAKTGDVKEILMAvLPCMlsyYYIFKELVKKYKD--NLESNP-YKDWIE 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1537797680 183 LHEGDYFRAWTGRLIALVNE-YASVDGSE----ADVFRTIVHLERRFFE 226
Cdd:cd19369   153 DYASEEYAEYCKEWIDFADRlCENLSEEEkeklKEIFRKASLYELKFWD 201
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 35-229 1.78e-11

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 60.99  E-value: 1.78e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  35 DEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIelaprqetkdmLAKQSQWFAD------------NE 102
Cdd:cd19367     1 PIWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHAL-----------AAYKAPTLEDiraaaaillailEE 69

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 103 ATYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKL-VQGTWEELITAL--CCMEwiYLAWAKR-TIDAGVVQQIPAHK 178
Cdd:cd19367    70 MELHVKYCAEWGISEEELEATPESPATLAYTRYVLDVgLSGDLLDLLVALapCLLG--YGEIGLRlAADPATKLEGNPYW 147

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1537797680 179 GWVDLHEGDYFRAWTGRLIALVNEYASVDGSEA------DVFRTIVHLERRFFEDSY 229
Cdd:cd19367   148 SWIETYASDEYQEAVREGIELLEKLAAERPSPArleelvKIFATATRLEIGFWDMGL 204
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 25-226 4.61e-11

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 60.29  E-value: 4.61e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  25 VADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRQETKDMLAKQSQWFADNE 102
Cdd:cd19361     1 LSERLYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDyaKVFALGVAKAKDEEVMRFFADLINAILNEE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 103 ATYFTGFLKEYGITDEEYNNSEQTPANREYCEYLTKLVQ-GTWEELITA-LCCMeWIYLAWAKRTIDA-GVVQQIPAHKG 179
Cdd:cd19361    81 MDIHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYeGGIAEILAAiLSCS-WSYEYIAKKLVERyPAALEHEFYGE 159

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1537797680 180 WVDLHEGDYFRAWTGRLIALVNEYAsVDGSEA------DVFRTIVHLERRFFE 226
Cdd:cd19361   160 WVKGYSSEEYAEANQELIDLLDRLT-EDISEEqiekleEIFVNCSRYELKFWD 211
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 26-228 1.91e-09

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 55.53  E-value: 1.91e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMA--HAIELAPRQETKDMLakQSQWFADNEA 103
Cdd:cd19362     1 SENLKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLRAllRASSKAPLDKAIKIL--NSVFSGRDKG 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 104 TYFTGFL-KEYGITDEEYNNSEQTPANREYCEYLTKLVQGTWEELITA-LCCMeWIYLAWAKRTIDAgvvqQIPAHKGWV 181
Cdd:cd19362    79 MEVHKFLySELGITEDEIRRTGYNLVNYAYTRHLYYYSTLGWPQFLAAwAPCM-WGYSEIGKYVLNS----PNELYKTWA 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1537797680 182 DLHEGDYFRAWTGRLIALVNEYASVDGSEaDVFRTIVHLERRFFEDS 228
Cdd:cd19362   154 SFYASKDYKKRVEAILEALDSIEDTEDIK-NIFRNSVNFEIMFWDAA 199
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 45-226 1.57e-07

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 50.28  E-value: 1.57e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  45 FVNELLDDTLPESILKSYLIQDFKFFNQ--GIMAHAIELAPRQETKDMLAKQSQWFADNEATYFTGFLKEYGITDEEYNN 122
Cdd:cd19360    21 FVQGIAAGELPKEALIHYVQQDYEYLNAflKVYALAIAKSDTREDMRFFLEQIGFILNGESHAHQNLCEVAGVDYEELQG 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 123 SEQTPANREYCEYLTKLVQ-GTWEELITALCCMEWIYLAWAKRTIDAGVVQqiPAH--KGWVDLHEGDYFRAWTGRLIAL 199
Cdd:cd19360   101 APWAPTADHYIKHMYYAARtGDLGDILAALLPCPWTYVELAKRLIEEGKPT--PDNpfYEWIDFYADDEMDGLTDQLFAR 178

                         170       180       190
                  ....*....|....*....|....*....|..
gi 1537797680 200 VNEYA-SVDGSE----ADVFRTIVHLERRFFE 226
Cdd:cd19360   179 LDRLAeKASEEEreraKQAFLKSCQLEWRFWE 210
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 26-226 2.35e-05

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 43.77  E-value: 2.35e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  26 ADQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNqgIMAHAIELA----PRQETKDMLAkQSQWFADN 101
Cdd:cd19368     2 AKRLWKKNRDEALFALYHPFVVGLAAGNLPLDSFRHYISQDAHFLE--AFARAYELAeakaDDDEDKKAIR-ELRKGVLE 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 102 EATYFTGFLKEYGITDEEYNNSeqTPANREYCEYLTKLVQGTWEELITALC----CME---WIYLAWAKRTIDAgvvqqi 174
Cdd:cd19368    79 ELKLHDSYAEEWGVDLPKEVTP--DPATRKYTDFLLATASGKVKVAAYTLAamapCMRlyaFLGQELARALDDT------ 150

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1537797680 175 PAH--KGWVDLHEGDYFRAWTGRLIALVNEYA-SVDGSEADVFRTIVH----LERRFFE 226
Cdd:cd19368   151 EDHpyKKWIDTYSSQEFEALALQLEDLLDKLSaSLTGEELEALEKLYRramkLEVEFFA 209
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 27-226 6.79e-04

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 39.64  E-value: 6.79e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680  27 DQLYMSAADEWNQAINSRFVNELLDDTLPESILKSYLIQDFKFFNQGIMAHAIEL--APRQETKDMLAKQSQwfadNEAT 104
Cdd:cd19359     2 DKLWNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAakAPDPDLLAFLAAKIK----SYLD 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1537797680 105 YFTGFLKEYGITDEEY---NNSEQTPANREYCEYLTKLVQ-GTWEELITAL--CCMEWIYLawAKRTIDAGVVQQIPAHK 178
Cdd:cd19359    78 YAEDFLKTCHIKLGIPdvvDGVKPSPALKAYVDFERSVAEsEDWFYLLVAMipCIYLWYWL--ANQLNEDPSDKNTNFYK 155

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1537797680 179 GWVDlhEGDYFRAWTGRLIALVNEYASV---DGSEA-DVFRTIVHLERRFFE 226
Cdd:cd19359   156 TWIE--PNLPDPSSAKQLEFFLNANAAWskiDREKAnEIFRQAMQLEINFFN 205
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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