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Conserved domains on  [gi|1519586809|gb|AZB33863|]
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sugar transferase [Chryseobacterium bernardetii]

Protein Classification

sugar transferase( domain architecture ID 10493287)

sugar transferase catalyzes the transfer of a sugar from a donor such as UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate

EC:  2.7.8.-
Gene Ontology:  GO:0006486|GO:0016740
SCOP:  4007826

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Bac_transf pfam02397
Bacterial sugar transferase; This Pfam family represents a conserved region from a number of ...
 43-188 1.66e-48

Bacterial sugar transferase; This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.


:

Pssm-ID: 460547 [Multi-domain]  Cd Length: 180  Bit Score: 155.59  E-value: 1.66e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT--INPKKGKSSS----------VGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGYY 110
Cdd:pfam02397  36 FFRQERVGKNGKPFTIYKFRTmvVDAEKRGPLFklkndpritrVGRFLRKTSLDELPQLINVLKGDMSLVGPRPELPEFE 115

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1519586809 111 DTLKGDD-RKVLLLKPGLTSEASIKysnedvllkaqkdplSYNDNIIFPDKVKLNLNYLENISFKEDLRILWRTFFKIL 188
Cdd:pfam02397 116 YELYERDqRRRLSVKPGITGLAQVN---------------GGRSELSFEEKLELDLYYIENWSLWLDLKILLKTVKVVL 179
 
Name Accession Description Interval E-value
Bac_transf pfam02397
Bacterial sugar transferase; This Pfam family represents a conserved region from a number of ...
 43-188 1.66e-48

Bacterial sugar transferase; This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.


Pssm-ID: 460547 [Multi-domain]  Cd Length: 180  Bit Score: 155.59  E-value: 1.66e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT--INPKKGKSSS----------VGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGYY 110
Cdd:pfam02397  36 FFRQERVGKNGKPFTIYKFRTmvVDAEKRGPLFklkndpritrVGRFLRKTSLDELPQLINVLKGDMSLVGPRPELPEFE 115

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1519586809 111 DTLKGDD-RKVLLLKPGLTSEASIKysnedvllkaqkdplSYNDNIIFPDKVKLNLNYLENISFKEDLRILWRTFFKIL 188
Cdd:pfam02397 116 YELYERDqRRRLSVKPGITGLAQVN---------------GGRSELSFEEKLELDLYYIENWSLWLDLKILLKTVKVVL 179
WcaJ COG2148
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane ...
 43-188 2.61e-47

Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441751 [Multi-domain]  Cd Length: 322  Bit Score: 157.21  E-value: 2.61e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKTINPKKGKSSS-------------VGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGY 109
Cdd:COG2148   174 FFRQERVGRNGRPFTIYKFRTMRVDAEKLLGavfklkndpritrVGRFLRKTSLDELPQLWNVLKGDMSLVGPRPELPEE 253

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 110 YDTLKGDD-RKVLLLKPGLTSEASIKYSNEdvllkaqkdplsyndnIIFPDKVKLNLNYLENISFKEDLRILWRTFFKIL 188
Cdd:COG2148   254 VELYEEEEyRRRLLVKPGITGLAQVNGRNG----------------ETFEERVELDLYYIENWSLWLDLKILLKTVLVVL 317
EPS_sugtrans TIGR03025
exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family ...
 43-188 1.87e-30

exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.


Pssm-ID: 274398 [Multi-domain]  Cd Length: 445  Bit Score: 114.99  E-value: 1.87e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT--INPKKGKSS-----------SVGLVLRKYKLDELPQLFNILKGDMSFVGPRP----D 105
Cdd:TIGR03025 293 FFRQERVGLNGKPFTVYKFRSmrVDAEEGGGPvqatkndpritRVGRFLRRTSLDELPQLFNVLKGDMSLVGPRPerpaE 372

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 106 IEGYYDTLKGDDRKvLLLKPGLTSEASIKysnedvllkaqkdplSYNDNIIFPDKVKLNLNYLENISFKEDLRILWRTFF 185
Cdd:TIGR03025 373 VEKYEQEIPGYMLR-HKVKPGITGWAQVS---------------GRGETSTMEERVEYDLYYIENWSLWLDLKILLKTVK 436


                  ...
gi 1519586809 186 KIL 188
Cdd:TIGR03025 437 VVL 439
PRK10124 PRK10124
putative UDP-glucose lipid carrier transferase; Provisional
 43-186 2.68e-15

putative UDP-glucose lipid carrier transferase; Provisional


Pssm-ID: 182254 [Multi-domain]  Cd Length: 463  Bit Score: 72.83  E-value: 2.68e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT----------INPKKGKS--SSVGLVLRKYKLDELPQLFNILKGDMSFVGPRPD----- 105
Cdd:PRK10124  308 IFRQTRYGMDGKPIKVWKFRSmkvmendkvvTQATQNDPrvTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHavahn 387

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 106 ------IEGYYDTLKgddrkvllLKPGLTSEASIK-YSNE-DVLLKAQKdplsyndniifpdKVKLNLNYLENISFKEDL 177
Cdd:PRK10124  388 eqyrqlIEGYMLRHK--------VKPGITGWAQINgWRGEtDTLEKMEK-------------RVEFDLEYIREWSVWFDI 446


                  ....*....
gi 1519586809 178 RILWRTFFK 186
Cdd:PRK10124  447 KIVFLTVFK 455
 
Name Accession Description Interval E-value
Bac_transf pfam02397
Bacterial sugar transferase; This Pfam family represents a conserved region from a number of ...
 43-188 1.66e-48

Bacterial sugar transferase; This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.


Pssm-ID: 460547 [Multi-domain]  Cd Length: 180  Bit Score: 155.59  E-value: 1.66e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT--INPKKGKSSS----------VGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGYY 110
Cdd:pfam02397  36 FFRQERVGKNGKPFTIYKFRTmvVDAEKRGPLFklkndpritrVGRFLRKTSLDELPQLINVLKGDMSLVGPRPELPEFE 115

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1519586809 111 DTLKGDD-RKVLLLKPGLTSEASIKysnedvllkaqkdplSYNDNIIFPDKVKLNLNYLENISFKEDLRILWRTFFKIL 188
Cdd:pfam02397 116 YELYERDqRRRLSVKPGITGLAQVN---------------GGRSELSFEEKLELDLYYIENWSLWLDLKILLKTVKVVL 179
WcaJ COG2148
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane ...
 43-188 2.61e-47

Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441751 [Multi-domain]  Cd Length: 322  Bit Score: 157.21  E-value: 2.61e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKTINPKKGKSSS-------------VGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGY 109
Cdd:COG2148   174 FFRQERVGRNGRPFTIYKFRTMRVDAEKLLGavfklkndpritrVGRFLRKTSLDELPQLWNVLKGDMSLVGPRPELPEE 253

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 110 YDTLKGDD-RKVLLLKPGLTSEASIKYSNEdvllkaqkdplsyndnIIFPDKVKLNLNYLENISFKEDLRILWRTFFKIL 188
Cdd:COG2148   254 VELYEEEEyRRRLLVKPGITGLAQVNGRNG----------------ETFEERVELDLYYIENWSLWLDLKILLKTVLVVL 317
EPS_sugtrans TIGR03025
exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family ...
 43-188 1.87e-30

exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase; Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.


Pssm-ID: 274398 [Multi-domain]  Cd Length: 445  Bit Score: 114.99  E-value: 1.87e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT--INPKKGKSS-----------SVGLVLRKYKLDELPQLFNILKGDMSFVGPRP----D 105
Cdd:TIGR03025 293 FFRQERVGLNGKPFTVYKFRSmrVDAEEGGGPvqatkndpritRVGRFLRRTSLDELPQLFNVLKGDMSLVGPRPerpaE 372

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 106 IEGYYDTLKGDDRKvLLLKPGLTSEASIKysnedvllkaqkdplSYNDNIIFPDKVKLNLNYLENISFKEDLRILWRTFF 185
Cdd:TIGR03025 373 VEKYEQEIPGYMLR-HKVKPGITGWAQVS---------------GRGETSTMEERVEYDLYYIENWSLWLDLKILLKTVK 436


                  ...
gi 1519586809 186 KIL 188
Cdd:TIGR03025 437 VVL 439
WcaJ_sugtrans TIGR03023
Undecaprenyl-phosphate glucose phosphotransferase; This family of proteins encompasses the E. ...
 43-188 1.01e-25

Undecaprenyl-phosphate glucose phosphotransferase; This family of proteins encompasses the E. coli WcaJ protein involved in colanic acid biosynthesis, the Methylobacillus EpsB protein involved in methanolan biosynthesis, as well as the GumD protein involved in the biosynthesis of xanthan. All of these are closely related to the well-characterized WbaP (formerly RfbP) protein, which is the first enzyme in O-antigen biosynthesis in Salmonella typhimurium. The enzyme transfers galactose from UDP-galactose (NOTE: not glucose) to a polyprenyl carrier (utilizing the highly conserved C-terminal sugar transferase domain, pfam02397) a reaction which takes place at the cytoplasmic face of the inner membrane. The N-terminal hydrophobic domain is then believed to facilitate the "flippase" function of transferring the liposaccharide unit from the cytoplasmic face to the periplasmic face of the inner membrane. Most of these genes are found within large operons dedicated to the production of complex exopolysaccharides such as the enterobacterial O-antigen. Colanic acid biosynthesis utilizes a glucose-undecaprenyl carrier, knockout of EpsB abolishes incorporation of UDP-glucose into the lipid phase, and the C-terminal portion of GumD has been shown to be responsible for the glucosyl-1-transferase activity.


Pssm-ID: 274396 [Multi-domain]  Cd Length: 450  Bit Score: 102.28  E-value: 1.01e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFF-QKRVGQNGKLFIIYKLKTINP----------KKGKS--SSVGLVLRKYKLDELPQLFNILKGDMSFVGPRP----- 104
Cdd:TIGR03023 294 VLFrQERYGLDGRPFMVYKFRSMRVhaegdgvtqaTRNDPrvTRVGAFLRRTSLDELPQFFNVLKGDMSIVGPRPhavah 373

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 105 ------DIEGYYdtLKgddRKVlllKPGLTSEASIK-YSNE-DVLLKAQKdplsyndniifpdKVKLNLNYLENISFKED 176
Cdd:TIGR03023 374 neqyrkLIPGYM--LR---HKV---KPGITGWAQVNgLRGEtDTLEKMEK-------------RVEYDLYYIENWSLWLD 432

                         170
                  ....*....|..
gi 1519586809 177 LRILWRTFFKIL 188
Cdd:TIGR03023 433 LKIILLTVFKGF 444
EpsB_2 TIGR03013
sugar transferase, PEP-CTERM system associated; Members of this protein family belong to the ...
 43-188 2.18e-24

sugar transferase, PEP-CTERM system associated; Members of this protein family belong to the family of bacterial sugar transferases (pfam02397). Nearly all are found in species that encode the PEP-CTERM/exosortase system predicted to act in protein sorting in a number of Gram-negative bacteria (notable exceptions appear to include Magnetococcus sp. MC-1 and Myxococcus xanthus DK 1622 ). These genes are generally found near one or more of the PrsK, PrsR or PrsT genes that have been related to the PEP-CTERM system by phylogenetic profiling methods. The nature of the sugar transferase reaction catalyzed by members of this clade is unknown and may conceivably be variable with respect to substrate by species. These proteins are homologs of the EpsB protein found in Methylobacillus sp. strain 12S, which is also associated with a PEP-CTERM system, but of a distinct type. A name which appears attached to a number of genes (by transitive annotation) in this family is "undecaprenyl-phosphate galactose phosphotransferase", which comes from relatively distant characterized enterobacterial homologs, and is considerably more specific than warranted from the currently available evidence.


Pssm-ID: 274390 [Multi-domain]  Cd Length: 442  Bit Score: 98.61  E-value: 2.18e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFF-QKRVGQNGKLFIIYKLKTINPKKGKS------------SSVGLVLRKYKLDELPQLFNILKGDMSFVGPRPDIEGY 109
Cdd:TIGR03013 290 VLYrQERVGLNGRPFNLIKFRSMRADAEKNgavwaqkddprvTRVGRFLRKTRIDELPQIFNVLRGDMSFVGPRPERPEF 369

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 110 YDTLK------GDDRKVlllKPGLTSEASIKY----SNEDVLLKAQKDplsyndniifpdkvklnLNYLENISFKEDLRI 179
Cdd:TIGR03013 370 VEKLSeeipyyNERHRV---KPGITGWAQIKYpygaSVADAKEKLRYD-----------------LYYIKNMSLLLDLII 429


                  ....*....
gi 1519586809 180 LWRTFFKIL 188
Cdd:TIGR03013 430 LIQTFEVVL 438
PRK10124 PRK10124
putative UDP-glucose lipid carrier transferase; Provisional
 43-186 2.68e-15

putative UDP-glucose lipid carrier transferase; Provisional


Pssm-ID: 182254 [Multi-domain]  Cd Length: 463  Bit Score: 72.83  E-value: 2.68e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  43 IFFQKRVGQNGKLFIIYKLKT----------INPKKGKS--SSVGLVLRKYKLDELPQLFNILKGDMSFVGPRPD----- 105
Cdd:PRK10124  308 IFRQTRYGMDGKPIKVWKFRSmkvmendkvvTQATQNDPrvTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHavahn 387

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809 106 ------IEGYYDTLKgddrkvllLKPGLTSEASIK-YSNE-DVLLKAQKdplsyndniifpdKVKLNLNYLENISFKEDL 177
Cdd:PRK10124  388 eqyrqlIEGYMLRHK--------VKPGITGWAQINgWRGEtDTLEKMEK-------------RVEFDLEYIREWSVWFDI 446


                  ....*....
gi 1519586809 178 RILWRTFFK 186
Cdd:PRK10124  447 KIVFLTVFK 455
PRK15204 PRK15204
undecaprenyl-phosphate galactose phosphotransferase; Provisional
 42-183 6.60e-15

undecaprenyl-phosphate galactose phosphotransferase; Provisional


Pssm-ID: 185126 [Multi-domain]  Cd Length: 476  Bit Score: 71.96  E-value: 6.60e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  42 GIFFQKRVGQNGKLFIIYKLKTI--------------NP-------------KKGKSSSVGLVLRKYKLDELPQLFNILK 94
Cdd:PRK15204  313 AIYGHQRVGRHGKLFPCYKFRSMvmnsqevlkellanDPiaraewekdfklkNDPRITAVGRFIRKTSLDELPQLFNVLK 392

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519586809  95 GDMSFVGPRPDIEGYYDTLKGDDRKVLLLKPGLTSEASIKYSNEdvllkaqkdpLSYNDNIIFPDKvklnlnYLENISFK 174
Cdd:PRK15204  393 GDMSLVGPRPIVSDELERYCDDVDYYLMAKPGMTGLWQVSGRND----------VDYDTRVYFDSW------YVKNWTLW 456


                  ....*....
gi 1519586809 175 EDLRILWRT 183
Cdd:PRK15204  457 NDIAILFKT 465
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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