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Conserved domains on  [gi|1421734212|gb|AXB56426|]
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cysteine methyltransferase [Flavobacterium fluviale]

Protein Classification

bifunctional helix-turn-helix domain-containing protein/methylated-DNA--[protein]-cysteine S-methyltransferase( domain architecture ID 11454393)

bifunctional helix-turn-helix domain-containing protein/methylated-DNA--[protein]-cysteine S-methyltransferase with similarity to bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada that has a functional switch mechanism from a DNA methyltransferase to a transcriptional regulator

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 117-280 2.03e-61

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


:

Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 191.62  E-value: 2.03e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 117 INYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNASfTRKLDLAQQNALFIFQNDwRKLSEIKLHLKGTD 196
Cdd:COG0350     2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAALRED-PPLLAEAARQLDAYFAGE-RKDFDLPLDLRGTP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 197 FQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEGA 276
Cdd:COG0350    80 FQRRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGA 159


                  ....
gi 1421734212 277 QVNQ 280
Cdd:COG0350   160 LAAA 163
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
28-116 4.26e-23

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


:

Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 95.23  E-value: 4.26e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  28 NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVNIEGMTPAE 107
Cdd:COG2207   170 TLEELARELGLSPRTLSRLFKEETGTSPKQYLRELRLERAKRLLAETDLSISEIAYELGFSSQSHFSRAFKKRFGVTPSE 249


                  ....*....
gi 1421734212 108 YKNGGKNLE 116
Cdd:COG2207   250 YRKRLRARA 258
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 117-280 2.03e-61

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 191.62  E-value: 2.03e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 117 INYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNASfTRKLDLAQQNALFIFQNDwRKLSEIKLHLKGTD 196
Cdd:COG0350     2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAALRED-PPLLAEAARQLDAYFAGE-RKDFDLPLDLRGTP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 197 FQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEGA 276
Cdd:COG0350    80 FQRRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGA 159


                  ....
gi 1421734212 277 QVNQ 280
Cdd:COG0350   160 LAAA 163
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
24-277 1.47e-48

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 164.58  E-value: 1.47e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  24 KEQP-NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRaTVSETAYETGLSGTSRLHDLFVNIEG 102
Cdd:PRK15435   96 QETPvTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLREALAKGE-SVTTSILNAGFPDSSSYYRKADETLG 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 103 MTPAEYKNGGKNLEINYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNA-------SFTRKLdlAQQNAL 175
Cdd:PRK15435  175 MTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDAALISELQQMFPAAdnapadlTFQQHV--REVIAS 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 176 FifqNDWRKLSEIKLHLKGTDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSG 255
Cdd:PRK15435  253 L---NQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDG 329

                         250       260
                  ....*....|....*....|..
gi 1421734212 256 IFGGYMWGNTRKTAIIGWEGAQ 277
Cdd:PRK15435  330 ALSGYRWGVSRKAQLLRREAEN 351
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 196-276 1.25e-36

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 125.17  E-value: 1.25e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 196 DFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEG 275
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 1421734212 276 A 276
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 197-275 8.26e-35

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 120.28  E-value: 8.26e-35
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1421734212 197 FQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEG 275
Cdd:cd06445     1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 195-268 5.89e-32

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 113.18  E-value: 5.89e-32
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1421734212 195 TDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKT 268
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKE 74
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
28-116 4.26e-23

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 95.23  E-value: 4.26e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  28 NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVNIEGMTPAE 107
Cdd:COG2207   170 TLEELARELGLSPRTLSRLFKEETGTSPKQYLRELRLERAKRLLAETDLSISEIAYELGFSSQSHFSRAFKKRFGVTPSE 249


                  ....*....
gi 1421734212 108 YKNGGKNLE 116
Cdd:COG2207   250 YRKRLRARA 258
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
26-109 4.32e-21

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 84.91  E-value: 4.32e-21
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212   26 QPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVNIEGMTP 105
Cdd:smart00342   1 PLTLEDLAEALGVSPRHLQRLFKKETGTTPKQYLRDRRLERARRLLRDTDLSVTEIALRVGFSSQSYFSRAFKKLFGVTP 80


                   ....
gi 1421734212  106 AEYK 109
Cdd:smart00342  81 SEYR 84
HTH_18 pfam12833
Helix-turn-helix domain;
32-110 3.11e-19

Helix-turn-helix domain;


Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 79.94  E-value: 3.11e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  32 VAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRA-TVSETAYETGLSGTSRLHDLFVNIEGMTPAEYKN 110
Cdd:pfam12833   1 LAAALGMSPRTLSRLFKRELGLSPKEYLRRLRLERARRLLLEDTGlSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRR 80
PRK10371 PRK10371
transcriptional regulator MelR;
7-109 9.44e-11

transcriptional regulator MelR;


Pssm-ID: 182416 [Multi-domain]  Cd Length: 302  Bit Score: 60.99  E-value: 9.44e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212   7 INYnrIAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETG 86
Cdd:PRK10371  190 QFY--VSQMLGFIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAG 267

                          90       100
                  ....*....|....*....|...
gi 1421734212  87 LSGTSRLHDLFVNIEGMTPAEYK 109
Cdd:PRK10371  268 FRSSSRFYSTFGKYVGMSPQQYR 290
 
Name Accession Description Interval E-value
AdaB COG0350
DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, ...
 117-280 2.03e-61

DNA repair enzyme Ada (O6-methylguanine-DNA--protein-cysteine methyltransferase) [Replication, recombination and repair];


Pssm-ID: 440119 [Multi-domain]  Cd Length: 163  Bit Score: 191.62  E-value: 2.03e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 117 INYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNASfTRKLDLAQQNALFIFQNDwRKLSEIKLHLKGTD 196
Cdd:COG0350     2 IRYAIFDTPLGPLLIAATDRGLCALSFGDDREEALLARFPAALRED-PPLLAEAARQLDAYFAGE-RKDFDLPLDLRGTP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 197 FQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEGA 276
Cdd:COG0350    80 FQRRVWEALRKIPYGETVTYGELARAIGRPKAARAVGSACGANPIPIIIPCHRVIGADGSLGGYAGGLERKRALLELEGA 159


                  ....
gi 1421734212 277 QVNQ 280
Cdd:COG0350   160 LAAA 163
AdaA COG2169
Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), ...
 12-277 7.16e-56

Methylphosphotriester-DNA--protein-cysteine methyltransferase (N-terminal fragment of Ada), contains Zn-binding and two AraC-type DNA-binding domains [Replication, recombination and repair];


Pssm-ID: 441772 [Multi-domain]  Cd Length: 358  Bit Score: 183.72  E-value: 7.16e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  12 IAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRaTVSETAYETGLSGTS 91
Cdd:COG2169    86 VARACRLIEAGAEDRPSLEDLAARLGLSPRHLRRLFKAHTGVTPKAYARARRLLRARQLLQTGL-SVTDAAYAAGFGSLS 164

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  92 RLHDLFVNIEGMTPAEYKNGGKNLEINYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNASFT-RKLDLA 170
Cdd:COG2169   165 RFYEAFKKLLGMTPSAYRRGGAGAAIRFAPTPCSLGLLLVAASARGVCAILLGDDPEALLRDLQDRFPAAELIgGDAAFE 244

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 171 QQNALFIFQNDWRKLSEI-KLHLKGTDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHR 249
Cdd:COG2169   245 QLVAEVVGFVEGPLLGLDlPLDLRGTAFQQRVWQALRAIPAGETASYAEIAARIGAPKAVRAVAAACAANQLAVAIPCHR 324

                         250       260
                  ....*....|....*....|....*...
gi 1421734212 250 VIQSSGIFGGYMWGNTRKTAIIGWEGAQ 277
Cdd:COG2169   325 VVRADGALSGYRWGVERKRALLEREAAA 352
PRK15435 PRK15435
bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;
24-277 1.47e-48

bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada;


Pssm-ID: 185333 [Multi-domain]  Cd Length: 353  Bit Score: 164.58  E-value: 1.47e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  24 KEQP-NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRaTVSETAYETGLSGTSRLHDLFVNIEG 102
Cdd:PRK15435   96 QETPvTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLREALAKGE-SVTTSILNAGFPDSSSYYRKADETLG 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 103 MTPAEYKNGGKNLEINYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKFPNA-------SFTRKLdlAQQNAL 175
Cdd:PRK15435  175 MTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDAALISELQQMFPAAdnapadlTFQQHV--REVIAS 252

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 176 FifqNDWRKLSEIKLHLKGTDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSG 255
Cdd:PRK15435  253 L---NQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDG 329

                         250       260
                  ....*....|....*....|..
gi 1421734212 256 IFGGYMWGNTRKTAIIGWEGAQ 277
Cdd:PRK15435  330 ALSGYRWGVSRKAQLLRREAEN 351
DNA_binding_1 pfam01035
6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal ...
 196-276 1.25e-36

6-O-methylguanine DNA methyltransferase, DNA binding domain; This is the C-terminal DNA-binding domain of 6-O-methylguanine-DNA methyltransferases.


Pssm-ID: 460036  Cd Length: 81  Bit Score: 125.17  E-value: 1.25e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 196 DFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEG 275
Cdd:pfam01035   1 PFQRRVWEALRQIPYGKTTTYGEIAKLLGRPKAARAVGNALGANPIPIIVPCHRVVGSDGSLGGYAGGLERKRALLELEG 80


                  .
gi 1421734212 276 A 276
Cdd:pfam01035  81 V 81
ATase cd06445
The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT ...
 197-275 8.26e-35

The DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase; also known as AGT, AGAT and MGMT) reverses O6-alkylation DNA damage by transferring O6-alkyl adducts to an active site cysteine irreversibly, without inducing DNA strand breaks. ATases are specific for repair of guanines with O6-alkyl adducts, however human ATase is not limited to O6-methylguanine, repairing many other adducts at the O6-position of guanine as well. ATase is widely distributed among species. Most ATases have N- and C-terminal domains. The C-terminal domain contains the conserved active-site cysteine motif (PCHR), the O6-alkylguanine binding channel, and the helix-turn-helix (HTH) DNA-binding motif. The active site is located near the recognition helix of the HTH motif. While the C-terminal domain of ATase contains residues that are necessary for DNA binding and alkyl transfer, the function of the N-terminal domain is still unknown. Removal of the N-terminal domain abolishes the activity of the C-terminal domain, suggesting an important structural role for the N-terminal domain in orienting the C-terminal domain for proper catalysis. Some ATase C-terminal domain homologs are either single-domain proteins that lack an N-terminal domain, or have a tryptophan substituted in place of the acceptor cysteine (i.e. the motif PCHR is replaced by PWHR). ATase null mutant mice are viable, fertile, and have a normal lifespan.


Pssm-ID: 119438 [Multi-domain]  Cd Length: 79  Bit Score: 120.28  E-value: 8.26e-35
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1421734212 197 FQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGWEG 275
Cdd:cd06445     1 FQRRVWEALRQIPYGEVTTYGQIAKLAGTPKAARAVGSALARNPIPILIPCHRVVRSDGGLGGYRGGLERKRELLELEG 79
ogt TIGR00589
O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are ...
 195-268 5.89e-32

O-6-methylguanine DNA methyltransferase; All proteins in this family for which functions are known are involved alkyl-DNA transferases which remove alkyl groups from DNA as part of alkylation DNA repair. Some of the proteins in this family are also transcription regulators and have a distinct transcription regulatory domain. This family is based on the phylogenomic analysis of JA Eisen (1999, Ph.D. Thesis, Stanford University). [DNA metabolism, DNA replication, recombination, and repair]


Pssm-ID: 273157  Cd Length: 80  Bit Score: 113.18  E-value: 5.89e-32
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1421734212 195 TDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKT 268
Cdd:TIGR00589   1 TPFQQKVWKALRTIPYGETKSYGQLAKAIGNPKAARAVGGANGRNPLAILVPCHRVVGKNGTLTGYGGGLERKE 74
PRK00901 PRK00901
methylated-DNA--protein-cysteine methyltransferase; Provisional
 118-263 2.48e-28

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 234860 [Multi-domain]  Cd Length: 155  Bit Score: 106.29  E-value: 2.48e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 118 NYSFAESPFGNIIVASTQKGVCFMAFAEDETIGFVDLKNKfpnaSFTRKLDlAQQNALFIFQndwRKLSEIKLHLKGTDF 197
Cdd:PRK00901    3 NIYFYETPIGKIGIAENGTAITHLCFGEDKIPKDVTILET----DLLKEAN-KQLEEYFEGK---RKKFDLPLAPQGTEF 74

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1421734212 198 QLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWG 263
Cdd:PRK00901   75 QKKVWKALQEIPYGETRSYKEIAVNIGNPKACRAVGLANNKNPIPIFIPCHRVIGANGKLVGYAGG 140
PRK10286 PRK10286
methylated-DNA--[protein]-cysteine S-methyltransferase;
194-275 1.35e-23

methylated-DNA--[protein]-cysteine S-methyltransferase;


Pssm-ID: 182355 [Multi-domain]  Cd Length: 171  Bit Score: 94.17  E-value: 1.35e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212 194 GTDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSGIFGGYMWGNTRKTAIIGW 273
Cdd:PRK10286   86 GTPFQREVWQTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRH 165


                  ..
gi 1421734212 274 EG 275
Cdd:PRK10286  166 EG 167
AraC COG2207
AraC-type DNA-binding domain and AraC-containing proteins [Transcription];
28-116 4.26e-23

AraC-type DNA-binding domain and AraC-containing proteins [Transcription];


Pssm-ID: 441809 [Multi-domain]  Cd Length: 258  Bit Score: 95.23  E-value: 4.26e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  28 NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVNIEGMTPAE 107
Cdd:COG2207   170 TLEELARELGLSPRTLSRLFKEETGTSPKQYLRELRLERAKRLLAETDLSISEIAYELGFSSQSHFSRAFKKRFGVTPSE 249


                  ....*....
gi 1421734212 108 YKNGGKNLE 116
Cdd:COG2207   250 YRKRLRARA 258
HTH_ARAC smart00342
helix_turn_helix, arabinose operon control protein;
26-109 4.32e-21

helix_turn_helix, arabinose operon control protein;


Pssm-ID: 197666 [Multi-domain]  Cd Length: 84  Bit Score: 84.91  E-value: 4.32e-21
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212   26 QPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVNIEGMTP 105
Cdd:smart00342   1 PLTLEDLAEALGVSPRHLQRLFKKETGTTPKQYLRDRRLERARRLLRDTDLSVTEIALRVGFSSQSYFSRAFKKLFGVTP 80


                   ....
gi 1421734212  106 AEYK 109
Cdd:smart00342  81 SEYR 84
GlxA COG4977
Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH ...
 11-108 1.39e-20

Transcriptional regulator GlxA, contains an amidase domain and an AraC-type DNA-binding HTH domain [Transcription];


Pssm-ID: 444002 [Multi-domain]  Cd Length: 318  Bit Score: 89.45  E-value: 1.39e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  11 RIAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGT 90
Cdd:COG4977   211 RLARAQAWMEANLEEPLSVDELARRAGMSPRTLERRFRAATGTTPARYLQRLRLERARRLLETTDLSIEEIAAACGFGSA 290

                          90
                  ....*....|....*...
gi 1421734212  91 SRLHDLFVNIEGMTPAEY 108
Cdd:COG4977   291 SHFRRAFRRRFGVSPSAY 308
HTH_18 pfam12833
Helix-turn-helix domain;
32-110 3.11e-19

Helix-turn-helix domain;


Pssm-ID: 432818 [Multi-domain]  Cd Length: 81  Bit Score: 79.94  E-value: 3.11e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  32 VAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRA-TVSETAYETGLSGTSRLHDLFVNIEGMTPAEYKN 110
Cdd:pfam12833   1 LAAALGMSPRTLSRLFKRELGLSPKEYLRRLRLERARRLLLEDTGlSVAEIALALGFSDASHFSRAFRRLFGLTPSEYRR 80
Atl1 COG3695
Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like ...
 195-266 1.05e-12

Alkylated DNA nucleotide flippase Atl1, participates in nucleotide excision repair, Ada-like DNA-binding domain [Transcription];


Pssm-ID: 442910  Cd Length: 104  Bit Score: 62.90  E-value: 1.05e-12
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1421734212 195 TDFQLKVWETLLKIPMGQLSTYGSIAQQIEKPNASRAVGTAIGSNPVAFLIPCHRVIQSSG-IFGGYMWGNTR 266
Cdd:COG3695     4 EEFYERVYEVVAQIPPGRVATYGDIAALAGLPRGARQVGRALRALPEGSDLPWHRVVNADGrLSPGHAGGAEE 76
PRK10371 PRK10371
transcriptional regulator MelR;
7-109 9.44e-11

transcriptional regulator MelR;


Pssm-ID: 182416 [Multi-domain]  Cd Length: 302  Bit Score: 60.99  E-value: 9.44e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212   7 INYnrIAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETG 86
Cdd:PRK10371  190 QFY--VSQMLGFIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAG 267

                          90       100
                  ....*....|....*....|...
gi 1421734212  87 LSGTSRLHDLFVNIEGMTPAEYK 109
Cdd:PRK10371  268 FRSSSRFYSTFGKYVGMSPQQYR 290
PRK03887 PRK03887
methylated-DNA--protein-cysteine methyltransferase; Provisional
 195-256 1.29e-09

methylated-DNA--protein-cysteine methyltransferase; Provisional


Pssm-ID: 167628 [Multi-domain]  Cd Length: 175  Bit Score: 56.28  E-value: 1.29e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1421734212 195 TDFQLKVWETLLK-IPMGQLSTYGSIAQQIEkpNASRAVGTAIGSNPVAFLIPCHRVIQSSGI 256
Cdd:PRK03887   91 TPFERKVYEWLTKnVKRGEVITYGELAKALN--TSPRAVGGAMKRNPYPIIVPCHRVVGRKNP 151
ftrA PRK09393
transcriptional activator FtrA; Provisional
 11-109 4.46e-09

transcriptional activator FtrA; Provisional


Pssm-ID: 181818 [Multi-domain]  Cd Length: 322  Bit Score: 56.13  E-value: 4.46e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  11 RIAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGL--S 88
Cdd:PRK09393  219 RLGPLIDWMRAHLAEPHTVASLAARAAMSPRTFLRRFEAATGMTPAEWLLRERLARARDLLESSALSIDQIAERAGFgsE 298

                          90       100
                  ....*....|....*....|.
gi 1421734212  89 GTSRLHdlFVNIEGMTPAEYK 109
Cdd:PRK09393  299 ESLRHH--FRRRAATSPAAYR 317
PRK10572 PRK10572
arabinose operon transcriptional regulator AraC;
10-119 3.47e-07

arabinose operon transcriptional regulator AraC;


Pssm-ID: 236717 [Multi-domain]  Cd Length: 290  Bit Score: 50.36  E-value: 3.47e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  10 NRIAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSG 89
Cdd:PRK10572  183 PRVREACQYISDHLASEFDIESVAQHVCLSPSRLAHLFRQQLGISVLRWREDQRISRAKLLLQTTRMPIATIGRNVGYDD 262

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1421734212  90 T---SRlhdLFVNIEGMTPAEYKNGgkNLEINY 119
Cdd:PRK10572  263 QlyfSR---VFKKCTGASPSEFRAR--CEEKNN 290
HTH_AraC pfam00165
Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the ...
 19-60 1.06e-05

Bacterial regulatory helix-turn-helix proteins, AraC family; In the absence of arabinose, the N-terminal arm of AraC binds to the DNA binding domain (pfam00165) and helps to hold the two DNA binding domains in a relative orientation that favours DNA looping. In the presence of arabinose, the arms bind over the arabinose on the dimerization domain, thus freeing the DNA-binding domains. The freed DNA-binding domains are then able to assume a conformation suitable for binding to the adjacent DNA sites that are utilized when AraC activates transcription, and hence AraC ceases looping the DNA when arabinose is added.


Pssm-ID: 425497 [Multi-domain]  Cd Length: 42  Bit Score: 41.76  E-value: 1.06e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1421734212  19 IKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQ 60
Cdd:pfam00165   1 LRENLSTNLTIADIADELGFSRSYFSRLFKKYTGVTPSQYRH 42
PRK13500 PRK13500
HTH-type transcriptional activator RhaR;
16-110 8.88e-05

HTH-type transcriptional activator RhaR;


Pssm-ID: 184091 [Multi-domain]  Cd Length: 312  Bit Score: 43.17  E-value: 8.88e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  16 IDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHD 95
Cdd:PRK13500  212 ITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSV 291

                          90
                  ....*....|....*
gi 1421734212  96 LFVNIEGMTPAEYKN 110
Cdd:PRK13500  292 VFTRETGMTPSQWRH 306
PRK11511 PRK11511
MDR efflux pump AcrAB transcriptional activator MarA;
12-109 1.61e-04

MDR efflux pump AcrAB transcriptional activator MarA;


Pssm-ID: 236920 [Multi-domain]  Cd Length: 127  Bit Score: 40.47  E-value: 1.61e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  12 IAEAIDYIKANFKEQPNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTS 91
Cdd:PRK11511   11 IHSILDWIEDNLESPLSLEKVSERSGYSKWHLQRMFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQ 90

                          90
                  ....*....|....*...
gi 1421734212  92 RLHDLFVNIEGMTPAEYK 109
Cdd:PRK11511   91 TLTRTFKNYFDVPPHKYR 108
PRK13501 PRK13501
HTH-type transcriptional activator RhaR;
19-109 2.55e-04

HTH-type transcriptional activator RhaR;


Pssm-ID: 184092 [Multi-domain]  Cd Length: 290  Bit Score: 41.81  E-value: 2.55e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  19 IKANFkeqpNLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFV 98
Cdd:PRK13501  189 LGAYF----DMADFCHKNQLVERSLKQLFRQQTGMSISHYLRQIRLCHAKCLLRGSEHRISDIAARCGFEDSNYFSAVFT 264

                          90
                  ....*....|.
gi 1421734212  99 NIEGMTPAEYK 109
Cdd:PRK13501  265 REAGMTPRDYR 275
PRK13502 PRK13502
HTH-type transcriptional activator RhaR;
21-110 4.68e-04

HTH-type transcriptional activator RhaR;


Pssm-ID: 184093 [Multi-domain]  Cd Length: 282  Bit Score: 40.81  E-value: 4.68e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1421734212  21 ANFKEQP-NLDEVAEKVHLSPFHFQRLFSEWAGTSPKKFLQYTSIEHAKKLLKENRATVSETAYETGLSGTSRLHDLFVN 99
Cdd:PRK13502  186 ANSLECPfALDAFCQQEQCSERVLRQQFRAQTGMTINQYLRQVRICHAQYLLQHSPLMISEISMQCGFEDSNYFSVVFTR 265

                          90
                  ....*....|.
gi 1421734212 100 IEGMTPAEYKN 110
Cdd:PRK13502  266 ETGMTPSQWRH 276
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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