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Conserved domains on  [gi|1331402499|gb|AUR50568|]
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3-aminobutyryl-CoA ammonia lyase [Porphyromonas gingivalis ATCC 33277]

Protein Classification

hotdog fold domain-containing protein( domain architecture ID 10130798)

hotdog fold domain-containing protein belonging to the hotdog fold superfamily of thioesterases and dehydratases, similar to beta-alanyl-CoA:ammonia lyase that catalyzes the deamination of beta-alanyl-CoA to reversibly form ammonia and acrylyl-CoA

CATH:  3.10.129.10
Gene Ontology:  GO:0016836
PubMed:  15307895

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YciA super family cl43465
Acyl-CoA hydrolase [Lipid transport and metabolism];
54-97 3.34e-05

Acyl-CoA hydrolase [Lipid transport and metabolism];


The actual alignment was detected with superfamily member COG1607:

Pssm-ID: 441215 [Multi-domain]  Cd Length: 146  Bit Score: 40.93  E-value: 3.34e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1331402499  54 DNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARkVIRPRTD 97
Cdd:COG1607    55 DSVDFLRPVRVGDIVELYARVVRVGRTSMEVGVEVW-AEDLRTG 97
hot_dog cd03440
The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl ...
 7-89 3.63e-05

The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated proteins also share the hotdog fold. These proteins have related, but distinct, catalytic activities that include metabolic roles such as thioester hydrolysis in fatty acid metabolism, and degradation of phenylacetic acid and the environmental pollutant 4-chlorobenzoate. This superfamily also includes the PaaI-like protein FapR, a non-catalytic bacterial homolog involved in transcriptional regulation of fatty acid biosynthesis.


:

Pssm-ID: 239524 [Multi-domain]  Cd Length: 100  Bit Score: 39.77  E-value: 3.63e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331402499   7 IRLRMSAHDAH-YGGNLVDGARMLQLFgDVATELLIMQDGDEGLFCAY--DNVEFLAPVFAGDYIEAVGEITHVGNTSRK 83
Cdd:cd03440     1 FVLRLTVTPEDiDGGGIVHGGLLLALA-DEAAGAAAARLGGRGLGAVTlsLDVRFLRPVRPGDTLTVEAEVVRVGRSSVT 79


                  ....*.
gi 1331402499  84 MKFEAR 89
Cdd:cd03440    80 VEVEVR 85
 
Name Accession Description Interval E-value
YciA COG1607
Acyl-CoA hydrolase [Lipid transport and metabolism];
54-97 3.34e-05

Acyl-CoA hydrolase [Lipid transport and metabolism];


Pssm-ID: 441215 [Multi-domain]  Cd Length: 146  Bit Score: 40.93  E-value: 3.34e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1331402499  54 DNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARkVIRPRTD 97
Cdd:COG1607    55 DSVDFLRPVRVGDIVELYARVVRVGRTSMEVGVEVW-AEDLRTG 97
hot_dog cd03440
The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl ...
 7-89 3.63e-05

The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated proteins also share the hotdog fold. These proteins have related, but distinct, catalytic activities that include metabolic roles such as thioester hydrolysis in fatty acid metabolism, and degradation of phenylacetic acid and the environmental pollutant 4-chlorobenzoate. This superfamily also includes the PaaI-like protein FapR, a non-catalytic bacterial homolog involved in transcriptional regulation of fatty acid biosynthesis.


Pssm-ID: 239524 [Multi-domain]  Cd Length: 100  Bit Score: 39.77  E-value: 3.63e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331402499   7 IRLRMSAHDAH-YGGNLVDGARMLQLFgDVATELLIMQDGDEGLFCAY--DNVEFLAPVFAGDYIEAVGEITHVGNTSRK 83
Cdd:cd03440     1 FVLRLTVTPEDiDGGGIVHGGLLLALA-DEAAGAAAARLGGRGLGAVTlsLDVRFLRPVRPGDTLTVEAEVVRVGRSSVT 79


                  ....*.
gi 1331402499  84 MKFEAR 89
Cdd:cd03440    80 VEVEVR 85
4HBT pfam03061
Thioesterase superfamily; This family contains a wide variety of enzymes, principally ...
 20-90 4.41e-05

Thioesterase superfamily; This family contains a wide variety of enzymes, principally thioesterases. This family includes 4HBT (EC 3.1.2.23) which catalyzes the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3. This family includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyze the hydrolysis of other long chain fatty acyl-CoA thioesters.


Pssm-ID: 427116 [Multi-domain]  Cd Length: 79  Bit Score: 39.16  E-value: 4.41e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1331402499  20 GNLVDGARMLQLFGDVATELLIMQDGDEGLFCAYD-NVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARK 90
Cdd:pfam03061   1 GGVVHGGVYLALADEAAGAAARRLGGSQQVVVVVElSIDFLRPARLGDRLTVEARVVRLGRTSAVVEVEVRD 72
BFIT_BACH cd03442
Brown fat-inducible thioesterase (BFIT). Brain acyl-CoA hydrolase (BACH). These enzymes ...
 41-104 2.12e-03

Brown fat-inducible thioesterase (BFIT). Brain acyl-CoA hydrolase (BACH). These enzymes deacylate long-chain fatty acids by hydrolyzing acyl-CoA thioesters to free fatty acids and CoA-SH. Eukaryotic members of this family are expressed in brain, testis, and brown adipose tissues. The archeal and eukaryotic members of this family have two tandem copies of the conserved hot dog fold, while most bacterial members have only one copy.


Pssm-ID: 239526 [Multi-domain]  Cd Length: 123  Bit Score: 35.62  E-value: 2.12e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1331402499  41 IMQDGDEGLF-CAY------------DNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARkVIRPRTDISESAAD 104
Cdd:cd03442    30 LLEWMDELAGiAAYrhaggrvvtasvDRIDFLKPVRVGDVVELSARVVYTGRTSMEVGVEVE-AEDPLTGERRLVTS 105
FadM COG0824
Acyl-CoA thioesterase FadM [Lipid transport and metabolism]; Acyl-CoA thioesterase FadM is ...
 26-120 3.85e-03

Acyl-CoA thioesterase FadM [Lipid transport and metabolism]; Acyl-CoA thioesterase FadM is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440586 [Multi-domain]  Cd Length: 139  Bit Score: 34.87  E-value: 3.85e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331402499  26 ARMLQLFGDVATELLI--------MQDGDEGLFCAYDNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARKvirprtd 97
Cdd:COG0824    26 ANYLRYFEEARTEFLRalglsyaeLEEEGIGLVVVEAEIDYLRPARYGDELTVETRVVRLGGSSLTFEYEIFR------- 98

                          90       100
                  ....*....|....*....|...
gi 1331402499  98 isesaaDVLDEPIVVCRATGTCV 120
Cdd:COG0824    99 ------ADDGELLATGETVLVFV 115
 
Name Accession Description Interval E-value
YciA COG1607
Acyl-CoA hydrolase [Lipid transport and metabolism];
54-97 3.34e-05

Acyl-CoA hydrolase [Lipid transport and metabolism];


Pssm-ID: 441215 [Multi-domain]  Cd Length: 146  Bit Score: 40.93  E-value: 3.34e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1331402499  54 DNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARkVIRPRTD 97
Cdd:COG1607    55 DSVDFLRPVRVGDIVELYARVVRVGRTSMEVGVEVW-AEDLRTG 97
hot_dog cd03440
The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl ...
 7-89 3.63e-05

The hotdog fold was initially identified in the E. coli FabA (beta-hydroxydecanoyl-acyl carrier protein (ACP)-dehydratase) structure and subsequently in 4HBT (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A number of other seemingly unrelated proteins also share the hotdog fold. These proteins have related, but distinct, catalytic activities that include metabolic roles such as thioester hydrolysis in fatty acid metabolism, and degradation of phenylacetic acid and the environmental pollutant 4-chlorobenzoate. This superfamily also includes the PaaI-like protein FapR, a non-catalytic bacterial homolog involved in transcriptional regulation of fatty acid biosynthesis.


Pssm-ID: 239524 [Multi-domain]  Cd Length: 100  Bit Score: 39.77  E-value: 3.63e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331402499   7 IRLRMSAHDAH-YGGNLVDGARMLQLFgDVATELLIMQDGDEGLFCAY--DNVEFLAPVFAGDYIEAVGEITHVGNTSRK 83
Cdd:cd03440     1 FVLRLTVTPEDiDGGGIVHGGLLLALA-DEAAGAAAARLGGRGLGAVTlsLDVRFLRPVRPGDTLTVEAEVVRVGRSSVT 79


                  ....*.
gi 1331402499  84 MKFEAR 89
Cdd:cd03440    80 VEVEVR 85
4HBT pfam03061
Thioesterase superfamily; This family contains a wide variety of enzymes, principally ...
 20-90 4.41e-05

Thioesterase superfamily; This family contains a wide variety of enzymes, principally thioesterases. This family includes 4HBT (EC 3.1.2.23) which catalyzes the final step in the biosynthesis of 4-hydroxybenzoate from 4-chlorobenzoate in the soil dwelling microbe Pseudomonas CBS-3. This family includes various cytosolic long-chain acyl-CoA thioester hydrolases. Long-chain acyl-CoA hydrolases hydrolyse palmitoyl-CoA to CoA and palmitate, they also catalyze the hydrolysis of other long chain fatty acyl-CoA thioesters.


Pssm-ID: 427116 [Multi-domain]  Cd Length: 79  Bit Score: 39.16  E-value: 4.41e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1331402499  20 GNLVDGARMLQLFGDVATELLIMQDGDEGLFCAYD-NVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARK 90
Cdd:pfam03061   1 GGVVHGGVYLALADEAAGAAARRLGGSQQVVVVVElSIDFLRPARLGDRLTVEARVVRLGRTSAVVEVEVRD 72
PaaI COG2050
Acyl-CoA thioesterase PaaI, contains HGG motif [Secondary metabolites biosynthesis, transport ...
 55-122 1.85e-03

Acyl-CoA thioesterase PaaI, contains HGG motif [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441653 [Multi-domain]  Cd Length: 138  Bit Score: 36.07  E-value: 1.85e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1331402499  55 NVEFLAPVFAGDYIEAVGEITHVGNTsrkmkfearkvirprtdISESAADVLDE-PIVVCRATGTCVVP 122
Cdd:COG2050    83 NINFLRPARLGDRLTAEARVVRRGRR-----------------LAVVEVEVTDEdGKLVATATGTFAVL 134
BFIT_BACH cd03442
Brown fat-inducible thioesterase (BFIT). Brain acyl-CoA hydrolase (BACH). These enzymes ...
 41-104 2.12e-03

Brown fat-inducible thioesterase (BFIT). Brain acyl-CoA hydrolase (BACH). These enzymes deacylate long-chain fatty acids by hydrolyzing acyl-CoA thioesters to free fatty acids and CoA-SH. Eukaryotic members of this family are expressed in brain, testis, and brown adipose tissues. The archeal and eukaryotic members of this family have two tandem copies of the conserved hot dog fold, while most bacterial members have only one copy.


Pssm-ID: 239526 [Multi-domain]  Cd Length: 123  Bit Score: 35.62  E-value: 2.12e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1331402499  41 IMQDGDEGLF-CAY------------DNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARkVIRPRTDISESAAD 104
Cdd:cd03442    30 LLEWMDELAGiAAYrhaggrvvtasvDRIDFLKPVRVGDVVELSARVVYTGRTSMEVGVEVE-AEDPLTGERRLVTS 105
FadM COG0824
Acyl-CoA thioesterase FadM [Lipid transport and metabolism]; Acyl-CoA thioesterase FadM is ...
 26-120 3.85e-03

Acyl-CoA thioesterase FadM [Lipid transport and metabolism]; Acyl-CoA thioesterase FadM is part of the Pathway/BioSystem: Menaquinone biosynthesis


Pssm-ID: 440586 [Multi-domain]  Cd Length: 139  Bit Score: 34.87  E-value: 3.85e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1331402499  26 ARMLQLFGDVATELLI--------MQDGDEGLFCAYDNVEFLAPVFAGDYIEAVGEITHVGNTSRKMKFEARKvirprtd 97
Cdd:COG0824    26 ANYLRYFEEARTEFLRalglsyaeLEEEGIGLVVVEAEIDYLRPARYGDELTVETRVVRLGGSSLTFEYEIFR------- 98

                          90       100
                  ....*....|....*....|...
gi 1331402499  98 isesaaDVLDEPIVVCRATGTCV 120
Cdd:COG0824    99 ------ADDGELLATGETVLVFV 115
MaoC COG2030
Acyl-CoA dehydratase PaaZ [Lipid transport and metabolism];
13-77 4.80e-03

Acyl-CoA dehydratase PaaZ [Lipid transport and metabolism];


Pssm-ID: 441633 [Multi-domain]  Cd Length: 140  Bit Score: 34.86  E-value: 4.80e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1331402499  13 AHDAHYGGNLVDG----ARMLQLFGDVATellimqdGDEGLFCAYDNVEFLAPVFAGDYIEAVGEITHV 77
Cdd:COG2030    43 AAATGFGGRIAHGmltlSLASGLLVDDLP-------GTAVANLGLQEVRFLRPVRVGDTLRARVEVLEK 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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