3CER,2J4R,1T6C


Conserved Protein Domain Family
ASKHA_NBD_AaPPX-GppA_MtPPX2-like
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cd24054: ASKHA_NBD_AaPPX-GppA_MtPPX2-like 
nucleotide-binding domain (NBD) of Aquifex aeolicus PPX/GppA, Mycobacterium tuberculosis PPX2, Fusobacterium nucleatum AroB, and similar proteins
The PPX/GppA family proteins play essential roles in bacterial survival and metabolism. Guanosine pentaphosphate (pppGpp) phosphohydrolase (GppA; EC 3.6.1.40) plays a key role in (p)ppGpp homeostasis. It specifically catalyzes the conversion of pppGpp to ppGpp (guanosine tetraphosphate). Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX; EC 3.6.1.11), which mediates the metabolism of cellular inorganic polyphosphate. Especially, it is responsible for the maintenance of appropriate levels of cellular inorganic polyphosphate (PolyP). The family corresponds to a group of proteins similar to Aquifex aeolicus exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (AaPPX/GppA), Mycobacterium tuberculosis exopolyphosphatase 2 (MtPPX2), Fusobacterium nucleatum bifunctional 3-dehydroquinate synthase/phosphatase (AroB) and similar proteins.
Links
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Statistics
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PSSM-Id: 466904
Aligned: 256 rows
Threshold Bit Score: 188.072
Created: 19-Dec-2022
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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catalytic sitenucleotideactive site
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:The four catalytic residues are conserved among PPX/GppA homologs.
  • Comment:The PPX/GPPA enzymes use metal ions for catalysis.
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1            #                                                                           
3CER_A        18 VAGIDCGTNSIRLKIARVdadgxhevVPRILRVIRLGqdvdktHRFADEALERAYVAAREFAGVIAehpiDGLRFVATSA 97  Bifidobacterium...
2J4R_A         9 VASIDIGSNSVRLTIAQIkdgk-lsiILERGRITSLGtkvketGRLQEDRIEETIQVLKEYKKLIDefkvERVKAVATEA 87  Aquifex aeolicus
OUW44038       8 VAAIDCGSNSTRLLIADVssge-lvsIFKTHKVTKTSeglegsNEISNDAKNRLIKALREYQKKINlenvDQIFITGTAV 86  Acidimicrobiace...
OUR71753       4 VTTIDLGSNSFRVLVYNYkkh---vvLKEYNEIVGTAddlintGLISNEAIHRVIGAINKSSSELDyn-pRNSICVTTAA 79  Arcobacter sp. ...
RUM58071       1 MVAIDLGSNTIRFVEYDCnkk---qiVNSYEEIVRTAdnliktKKISLEAIERIINAIKNAKEILNf---DEVKAISTEA 74  Nautilia sp.
OQX74226       1 MVACDLGSNTFRVVEFDCrkk---vrLKEYEKIVRLAeglsesGKISKAALQRLLKAIEEANGIFDfp-rRPHRCVATAA 76  Campylobacterac...
WP_067176156   1 MIGCDLGSNTLRIVKIDCqsk---arILAFERIVRTAkdlhitGRIGEQAKENIFNALHEASALFDfk-nERCFCVTTEA 76  Sulfurospirillu...
WP_089188019   2 VSSIDLGSNTLRICVMDEnk----niISSSEAIVGSArnlkkdGKLDNEAKKRIMSALEKFTHKYDl---CKSKAVATEA 74  Campylobacter s...
RZP25846       4 YVALDFGSNSTRVLIASVknge-inkIYKKHIVTKMAeetsssGRISKNAISRVMDALSFFFKEISrhevSEIYAIGTSA 82  Candidatus Acti...
KKR88280       7 FALIDLGSSTVKLNVYEYetk---nvFHKDVRTVNMAenfypkLLITEEAMERVLAVLKRYKKYLEgekiENIKLVTTGI 83  Candidatus Wolf...

Feature 1                                     #                         #      #                 
3CER_A        98 TRDAeNREEFEDEIErilGVRPEVIPGTEEADLSFLGATSVvnrd-dlpaPYLVVDLGGGSTELVIggdgvsapttqVQG 176 Bifidobacterium...
2J4R_A        88 IRRAkNAEEFLERVKrevGLVVEVITPEQEGRYAYLAVAYSlkp----egEVCVVDQGGGSTEYVFgkg------ykVRE 157 Aquifex aeolicus
OUW44038      87 FRDAqNSEKIIEEIKnklDLEXXVIXGEEEGFLTSLGVLSTnti----eeNFFIVDIGGRSTELIYdf-------ekRTN 155 Acidimicrobiace...
OUR71753      80 MRMAkNSDEVFKKVFtstGVKLNIIDGEEEARLTLLAIKHAlkrqklqseKFVLLDIGGGSVELTIhs-------nnQYI 152 Arcobacter sp. ...
RUM58071      75 LRQAeNKEEVINKIKeatNIEFEVIDASLEAYYTSLAASKCink---efkKMFLVDIGGGSTELIVynn-----rnkEIL 146 Nautilia sp.
OQX74226      77 MRLAkNGLQIREEILkktGLDFEIIDAKEEAELTRLAVQTRlrelgmrddSCVLLDLGGGSTELTLce-------geIHI 149 Campylobacterac...
WP_067176156  77 MRVAsNAQEILNEIEttfGLTFRIITGKEEAFLTSLAIENAlkregfdptIYALFDLGGGSTEFTFck-------ngDKQ 149 Sulfurospirillu...
WP_089188019  75 FRMAsDSDEFFREIYakfGIKFEIISGESEAKLTALAIKNRleklqiydkNALFIDLGGGSSEISF-----------GDN 143 Campylobacter s...
RZP25846      83 MRDSeNSLEIREVINeqfNVDVEIISGSEEAKLTLDGALCGfee----nlKTILFDIGGGSTEVIYtd-------qkNVS 151 Candidatus Acti...
KKR88280      84 ARKAkNSDLLVKKIKeilNWNLEIISGEKEAELFYLGVTNDfsp----dlRLAAINVGGGSTEVTIgtr------dsINK 153 Candidatus Wolf...

Feature 1                                                                                        
3CER_A       177 AFSXNIGSVRXTERHltndpp-tqtqiDEAVADVDEHIDeafrt----vdagKARTIIGVSGTVTTXTALAxglkeydht 251 Bifidobacterium...
2J4R_A       158 VISLPIGIVNLTETFfkqdpp-teeevKRFFEFLEKELSkv---------kkPVDTIVGLGGTITTLAALEynvypydpq 227 Aquifex aeolicus
OUW44038     156 VHSLDIGVVSLTERVantn-----pysSSDKEEADDLINknidl----eidtHQTFMIGVSGTFTSIASIFleqkiynee 226 Acidimicrobiace...
OUR71753     153 VKSFDFGIVTMTQKFqnd------silISELSKQTKKILsylnd---lnielSNFEFIATAGTPTTIAAVKhgqnfldyn 223 Arcobacter sp. ...
RUM58071     147 SESFKIGIVTISQKYksi------ealEYGIRKDTKEFKdfidl---ikityKPDIFVASSGTPTTIAALKiglnystyd 217 Nautilia sp.
OQX74226     150 DQSLDIGIVTILEEEsqngl---rrkiDEYYQPLAQFFNqslq-----kigsRPQYFVATAGTPTTIAAFLqgvdynhyd 221 Campylobacterac...
WP_067176156 150 SQSFPFGIINVAERYktdleqniasivGSIDGFIQKQKTti---------psNFLQLVTTAGTPTTVAAFLegldynhyd 220 Sulfurospirillu...
WP_089188019 144 FKSFKFGIVTFFNEFnslek--mqenaDCVVKNATKFIQk-----------fNFDKVVLTSGVPTTLAALKlkmdyqsyd 210 Campylobacter s...
RZP25846     152 PISYQLGVVRMSEDVfpsr-----pvsEIEESRAVELINslisiddmefsitDTTSAIGTAGTFTSLAAISlrldiydpe 226 Candidatus Acti...
KKR88280     154 RYSFPIGVSKLNEQFlqvd-----pptDAMIEEMFSAINevmefe--dnneyTPDVLIHTGGELDYMEITKhpleesfls 226 Candidatus Wolf...

Feature 1                                                                                        
3CER_A       252 --vvdghrlsfEDAYAVDDKFLRXTRAERReyk-tiHPGRIDVVGGGAVVWSRVLARVseaakadhgeaiDSFVASEhGL 328 Bifidobacterium...
2J4R_A       228 --kvhgkvltyGQIKKWFDTFKEIPSEERSkrfrqvEDRRAKVILAGIGIFLKTLEIFe----------kDCLIVSDwGL 295 Aquifex aeolicus
OUW44038     227 --eihltslsySWISNLSSKLNQMSEAQIIskypslDPKRAKTLSAGVLIVCNIMKKFk----------fDELKVSKsDI 294 Acidimicrobiace...
OUR71753     224 keivngtvinlSDINTCLSLLKNKSDFELNki---fGPNSKDFIKVGILIYKSFFEILn----------kTESTVFDdGL 290 Arcobacter sp. ...
RUM58071     218 pqkvnnteltiDDLDYWMDRLLKMDMKEREkl---vGIGRGDLIVSGIMIFKEIFRLSg----------fDKCIVCDmGV 284 Nautilia sp.
OQX74226     222 ykkingmvitrEMLHKALERLLKMGEEERRrw---vGVGREDLIIAGVLMLEKIIAICg----------yEEMIVIDdGL 288 Campylobacterac...
WP_067176156 221 anvvngkvlhiNDFEEAYERLTTMNLDEAEry---tGTNRRDLVIVGILIVKAIMKKLg----------fETCIVIDdGL 287 Sulfurospirillu...
WP_089188019 211 anlvngtklryEEIKNAINEISILDVSLATkl---lGKDRQILMIAGCILLTNMLKNFk----------dREFIVIDdGL 277 Campylobacter s...
RZP25846     227 --kthlhtiekSWLREFYYELRSLSPEEIInkysclDPARATTITPGALIALQLMEIFk----------iNKLVISEnDI 294 Candidatus Acti...
KKR88280     227 --pshpkqitkSKFLEKYNEIKKMPKSRLYefm-pqNPKWMDGAVACTAIALAIADKLg----------vNLIVPSNkNL 293 Candidatus Wolf...

Feature 1               
3CER_A       329 LDGIVLD 335 Bifidobacterium longum NCC2705
2J4R_A       296 REGVLVS 302 Aquifex aeolicus
OUW44038     295 LEGLILK 301 Acidimicrobiaceae bacterium TMED189
OUR71753     291 KEGVAIN 297 Arcobacter sp. 31_11_sub10_T18
RUM58071     285 REGVAFD 291 Nautilia sp.
OQX74226     289 REGVALK 295 Campylobacteraceae bacterium 4484_4
WP_067176156 288 REGVVLE 294 Sulfurospirillum sp. UCH001
WP_089188019 278 REGVCLY 284 Campylobacter sputorum
RZP25846     295 LEGLILN 301 Candidatus Actinomarinales bacterium
KKR88280     294 NDGLLLK 300 Candidatus Wolfebacteria bacterium GW2011_GWB1_41_12

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