Larval Sea Lamprey Liver - SRA

SRX109766: Larval Sea Lamprey Liver
1 LS454 (454 GS FLX) run: 155,729 spots, 24.9M bases, 14.4Mb downloads

Design: Total RNA was extracted using VersaGene RNA Tissue Kit (Gentra, Inc). The quality of the extracted RNA was verified by gel electrophoresis and quantified using a NanoDrop ND-1000 spectrophotometer. 1ug of total RNA was used as a template for first strand cDNA synthesis using the SMART™ cDNA Synthesis Kit (Clonetech Laboratories, Inc.). 13 cycles of LD-PCR was performed to amplify single-strand cDNA using the Advantage® 2 PCR Kit (Clonetech Laboratories, Inc.) according to manufacturer’s instructions. PCR products were purified using QIAquick PCR Purification Kit (Qiagen, Inc) and concentrated on Millipore YM-30 (MWCO 30,000) columns. 5200ng of cDNA were submitted to MSU RTSF for 454 sequencing.

Submitted by: MICHIGAN STATE UNIVERSITY

Study: Supplementary Sequencing Data

• SRP009480 • All experiments • All runs

show Abstracthide Abstract

Sequencing data from sea lamprey RNA using 454 and Illumina RNASeq.

Sample: Larval Sea Lamprey Liver

SAMN00761038 • SRS281247 • All experiments • All runs

Organism: Petromyzon marinus

Library:

Name: GS-FLX Li03 05/2008

Instrument: 454 GS FLX

Strategy: EST