Parasitic Sea Lamprey Olfactory Epithelium - SRA

SRX109761: Parasitic Sea Lamprey Olfactory Epithelium
1 LS454 (454 GS 20) run: 182,324 spots, 19.2M bases, 15.4Mb downloads

Design: Olfactory epithelia were flash frozen in liquid nitrogen and stored at -80*C until extraction. Total RNA was extracted using VersaGene RNA Tissue Kit (Gentra, Inc). The quality of the extracted RNA was verified by gel electrophoresis and quantified using a NanoDrop ND-1000 spectrophotometer. 1ug of total RNA was used as a template for first strand cDNA synthesis using the SMART™ cDNA Synthesis Kit (Clonetech Laboratories, Inc.). 13 cycles of LD-PCR was performed to amplify single-strand cDNA using the Advantage* 2 PCR Kit (Clonetech Laboratories, Inc.) according to manufacturer’s instructions. PCR products were purified using QIAquick PCR Purification Kit (Qiagen, Inc) and concentrated on Millipore YM-30 (MWCO 30,000) columns. 5200ng of cDNA were submitted to MSU RTSF for 454 sequencing.

Submitted by: MICHIGAN STATE UNIVERSITY

Study: Supplementary Sequencing Data

• SRP009480 • All experiments • All runs

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Sequencing data from sea lamprey RNA using 454 and Illumina RNASeq.

Sample: Parasitic Petromyzon marinus olfactory epithelium

SAMN00760978 • SRS281187 • All experiments • All runs

Organism: Petromyzon marinus

Library:

Name: GS20 04/2007 Lib1

Instrument: 454 GS 20

Strategy: EST