GSM1669051: JC1532_MCF7_Input_Full_Media_3hr r1; Homo sapiens; ChIP-S... - SRA

SRX1012634: GSM1669051: JC1532_MCF7_Input_Full_Media_3hr r1; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.8M spots, 952.7M bases, 614.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: ChIP-seq of ER, PR, p300 in two ER+ breast cancer cell lines treated with estrogen and with and without progestins

PRJNA282508 • SRP057755 • All experiments • All runs

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Exploring effect of progesterone/progestin treatment on ER and PR binding. Overall design: Two cell lines, three conditions (Full Media with E2, E2+ Progesterone, Full Media + R5020 Progestin), three factors (ER, PR, p300), all with three replicates, each with a matched Input control.

Sample: JC1532_MCF7_Input_Full_Media_3hr r1

SAMN03570661 • SRS924434 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Experiment attributes:

GEO Accession: GSM1669051

Links:

External link: GEO Sample GSM1669051

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 23.8M spots, 952.7M bases, 614.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR2000750	23,816,251	952.7M	614.1Mb	2015-05-22

ID:: 1467486

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