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SRX1012625: GSM1669042: JC1523_MCF7_ER_Full_Media_3hr r1; Homo sapiens; ChIP-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 23.1M spots, 922.1M bases, 558.2Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: ChIP-seq of ER, PR, p300 in two ER+ breast cancer cell lines treated with estrogen and with and without progestins
show Abstracthide Abstract
Exploring effect of progesterone/progestin treatment on ER and PR binding. Overall design: Two cell lines, three conditions (Full Media with E2, E2+ Progesterone, Full Media + R5020 Progestin), three factors (ER, PR, p300), all with three replicates, each with a matched Input control.
Sample: JC1523_MCF7_ER_Full_Media_3hr r1
SAMN03570650 • SRS924430 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations
Experiment attributes:
GEO Accession: GSM1669042
Links:
External link:
Runs: 2 runs, 23.1M spots, 922.1M bases, 558.2Mb
Run# of Spots# of BasesSizePublished
SRR200073212,416,425496.7M299.9Mb2015-05-22
SRR200073310,634,901425.4M258.3Mb2015-05-22

ID:
1467477

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