RNA-Seq of Cynoglossus semilaevis: disease tolerance lliver - SRA

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SRX24176456: RNA-Seq of Cynoglossus semilaevis: disease tolerance lliver
1 ILLUMINA (Illumina NovaSeq X) run: 39.1M spots, 10.8G bases, 3.4Gb downloads

Design: Total RNA of gills were isolated and purified using TRIzol reagent, quantified with NanoDrop ND-1000, and assessed for integrity with Bioanalyzer 2100. Poly(A) RNA was purified from 1g total RNA using Dynabeads Oligo (dT)25-61005, then fragmented with Magnesium RNA Fragmentation Module. Cleaved RNA fragments were reverse-transcribed with SuperScript II Reverse Transcriptase and synthesized into U-labeled second-stranded DNAs with E. coli DNA polymerase I, RNase H, and dUTP Solution. Adapters were ligated to the ends of each strand, followed by size selection with AMPureXP beads. After treatment with heat-labile UDG enzyme, ligated products were PCR amplified, and the final cDNA library had an average insert size of 30050 bp. Finally, paired-end sequencing (PE150) was performed on an Illumina Novaseq X.

Submitted by: Qingdao University

Study: Cynoglossus semilaevis Transcriptome or Gene expression

PRJNA1096978 • SRP500110 • All experiments • All runs

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Liver and spleen tissue transcriptome sequencing data from disease-sensitive and disease-tolerant individuals of half-smooth tongue sole (Cynoglossus semilaevis)

Sample:

SAMN40859932 • SRS20952128 • All experiments • All runs

Organism: Cynoglossus semilaevis

Library:

Name: DT-L1

Instrument: Illumina NovaSeq X

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 39.1M spots, 10.8G bases, 3.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR28576967	39,095,341	10.8G	3.4Gb	2024-04-07

ID:: 32487455

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