GSM1617801: BIRD1_gluc1; Pseudomonas putida BIRD-1; RNA-Seq - SRA

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SRX886503: GSM1617801: BIRD1_gluc1; Pseudomonas putida BIRD-1; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 5.7M spots, 566.7M bases, 304.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Genome-wide maps of the effect of butanol in P. putida BIRD1

PRJNA276156 • SRP055452 • All experiments • All runs

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We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic response of P. putida BIRD1 in the presence of butanol with the aim to study in more detail the mechanisms involved in butanol response. Our work is the first global transcriptomic analysis done in P. putida BIRD1 in the presence of butanol. Overall design: P. putida BIRD1 mRNA profiles in the presence of control condition (LB) and butanol (carbon source) or butanol shock.

Sample: BIRD1_gluc1

SAMN03366861 • SRS853407 • All experiments • All runs

Organism: Pseudomonas putida BIRD-1

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1617801

Links:

External link: GEO Sample GSM1617801

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 5.7M spots, 566.7M bases, 304.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR1814130	5,667,136	566.7M	304.8Mb	2016-04-08

ID:: 1273362

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