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SRX6433807: RNA-Seq of Sillago sihama: reoxygnation 4h-treated fish gill bioreplicate 1
1 ILLUMINA (Illumina HiSeq 2500) run: 23.5M spots, 7.1G bases, 2.2Gb downloads

Design: Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Leading Edge Genomic Services & Solutions Reverse Transcriptase(RNase H - ). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system
Submitted by: Guangdong Ocean University
Study: raw transcriptome sequence data of sillago sihama: hypoxia fish gill
show Abstracthide Abstract
Compared the transcriptome data of the hypoxic treatment group and the normal group to search hypoxia-related genes.
Sample:
SAMN12257704 • SRS5088412 • All experiments • All runs
Organism: Sillago sihama
Library:
Name: RO_G_1
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 23.5M spots, 7.1G bases, 2.2Gb
Run# of Spots# of BasesSizePublished
SRR967334423,503,8157.1G2.2Gb2020-08-09

ID:
8523958

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