RNA-Seq of Sillago sihama: hypoxia 1h-treated fish gill bioreplicate... - SRA

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SRX6433805: RNA-Seq of Sillago sihama: hypoxia 1h-treated fish gill bioreplicate 3
1 ILLUMINA (Illumina HiSeq 2500) run: 24M spots, 7.2G bases, 2.2Gb downloads

Design: Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Leading Edge Genomic Services & Solutions Reverse Transcriptase(RNase H - ). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system

Submitted by: Guangdong Ocean University

Study: raw transcriptome sequence data of sillago sihama: hypoxia fish gill

PRJNA552934 • SRP214401 • All experiments • All runs

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Compared the transcriptome data of the hypoxic treatment group and the normal group to search hypoxia-related genes.

Sample:

SAMN12257700 • SRS5088409 • All experiments • All runs

Organism: Sillago sihama

Library:

Name: HI_G_3

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 24M spots, 7.2G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR9673346	24,040,134	7.2G	2.2Gb	2020-08-09

ID:: 8523956

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