Arbutus unedo nuclear Restriction-Site Associated DNA (RAD) fragments... - SRA

SRX341237: Arbutus unedo nuclear Restriction-Site Associated DNA (RAD) fragments sequencing
1 ION_TORRENT (Ion Torrent PGM) run: 198,856 spots, 24.6M bases, 19.5Mb downloads

Design: Arbutus unedo DNA was extracted from purified nuclei and digested with restriction enzymes Csp6I and TasI (Fermentas). Subsequently, end repair of fragments, ligation of adapters and nick repair were carried out using the Ion Plus Fragment Library Kit (Life Technologies), being the fragments purified after each reaction using silica column based kits. Finally, fragments were separated by agarose gel electrophoresis and size-selection (200 to 400bp) was made by gel slicing followed by purification using the GeneJET Gell Extraction Kit (Fermentas).

Submitted by: UNIVERSIDADE DO ALGARVE

Study: Arbutus unedo Random survey

PRJNA217405 • SRP029321 • All experiments • All runs

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The goal of this study was to randomly obtain genomic sequences of Arbutus unedo for selecting and testing microsatellite markers. Strawberry tree located inside Gambelas Campus, University of Algarve, Faro, Portugal.

Sample: General Sample for Arbutus unedo

SAMN02338389 • SRS474415 • All experiments • All runs

Organism: Arbutus unedo

Library:

Name: A-unedo_LGGI

Instrument: Ion Torrent PGM

Strategy: OTHER

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Spot descriptor:

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Runs: 1 run, 198,856 spots, 24.6M bases, 19.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR959291	198,856	24.6M	19.5Mb	2013-08-31

ID:: 480955

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