XmnI digested monomers from Cercopithecus solatus - SRA

SRX1595681: XmnI digested monomers from Cercopithecus solatus
1 ION_TORRENT (Ion Torrent PGM) run: 204,990 spots, 30M bases, 22.9Mb downloads

Design: XmnI was used to digest Cercopithecus solatus DNA in vitro. 10 µg of Cercopithecus solatus genomic DNA were digested for 4 h 30 min at 37°C with 60 units of XmnI activity (New England Biolabs) in a total volume of 34 µL. The enzyme was inactivated for 20 min at 65°C. The sample was loaded on a 1% agarose gel after addition of 6.8 µL loading buffer (50% glycerol) and electrophoresis was performed in 0.5X Tris-borate-EDTA buffer, at room temperature for 2 h 45 min at 100 V. The gel was briefly stained with ethidium bromide and then imaged by UV transillumination. Band corresponding to alpha satellite monomers (~170 bp) was cut and DNA was extracted from the gel with the Omega Biotek Gel extraction kit and resuspended in 100 µl of elution buffer. About 220 ng were obtained.

Submitted by: National Museum of Natural History (France)

Study: Cercopithecini alpha satellite DNA

PRJNA311515 • SRP070633 • All experiments • All runs

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We have undertaken the targeted sequencing of the alpha satellite component of Cercopithecini species. Alpha satellite monomers and dimers were obtained by enzymatic digestion of genomic DNA and gel purification, then submitted to high throughput sequencing (Ion Torrent technology).

Sample: XmnI digested monomers from Cercopithecus solatus

SAMN04484901 • SRS1305791 • All experiments • All runs

Organism: Allochrocebus solatus

Library:

Instrument: Ion Torrent PGM

Strategy: OTHER

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 204,990 spots, 30M bases, 22.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR3180910	204,990	30M	22.9Mb	2016-02-22

ID:: 2250212

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