show Abstracthide AbstractNannochloropsis gaditana is a marine microalga widely used in various industrial applications as food and feed additive. It has been shown to be suitable for production of algal biofuel, due to its ease of growth and high oil content. It has also been shown to be suitable for genetic manipulation. Because of this traits, this microalga is now studied in many laboratories and is has become a model organism for investigating algal biofuel. In this project we sequenced the nuclear genome and the genomes of the organelles of Nannochloropsis gaditana strain B-31, which is the original strain isolated by Lubi n and conserved at the Instituto Espa ol de Oceanograf a (IEO). We assembled the genome almost at chromosome level and we provided an annotation of the genes based on both experimental evidence (RNA-Seq data) and gene prediction.