mRNA extracted from whole plant mock treated - SRA

SRX423870: mRNA extracted from whole plant mock treated
3 ILLUMINA (Illumina Genome Analyzer IIx) runs: 10.3M spots, 740.2M bases, 457.9Mb downloads

Design: In vitro-grown sugarcane plantlets were maintained at 28°C with an irradiance of 60 µmol photons m-2 s-1 and 12 h photoperiod. After the development of a root system, vigorous and pathogen-free plants were divided in two halves with a scalpel. One half was used as control, immersing the root system in distilled water by 5 minute and then washed twice with distilled water. The other half was inoculated by immersing the root system for 5 minute in a suspension of sugarcane pathogen bacteria in distilled water (106 CFU mL-1), and then washed twice with distilled water in order to eliminate superficial bacteria. Inoculated and mock plants were transferred to MS medium and kept there for seven days. After this period, whole plants were harvested and examined for bacterial colonization by plate counting. Total RNA was isolated from whole plants using Trizol (Invitrogen, CA, USA) as described by the manufacturer. The mRNA-seq library was constructed following the TruSeq RNA Sample Prep Kit.

Submitted by: UNIVERSIDADE FEDERAL DO RIO DE JANEIRO

Study: Saccharum hybrid cultivar cultivar:SP70-1143 Transcriptome or Gene expression

PRJNA226722 • SRP032773 • All experiments • All runs

show Abstracthide Abstract

The sequencing of sugarcane mRNA through the use of NGS in order to investigate the expressed transcripts in certain conditions.

Sample: Generic sample from Saccharum hybrid cultivar SP70-1143

SAMN02570586 • SRS527850 • All experiments • All runs

Organism: Saccharum hybrid cultivar SP70-1143

Library:

Name: MCS1

Instrument: Illumina Genome Analyzer IIx

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 3 runs, 10.3M spots, 740.2M bases, 457.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR1104746	3,268,660	235.3M	145.8Mb	2015-07-22
SRR1104748	3,516,354	253.2M	155.5Mb	2015-07-22
SRR1104749	3,496,199	251.7M	156.6Mb	2015-07-22

ID:: 599344

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity