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DRX233881: DNBSEQ-G400 paired end sequencing of SAMD00245770
1 DNBSEQ (DNBSEQ-G400) run: 9.9M spots, 3G bases, 2.1Gb downloads

Submitted by: GUNMA
Study: Characterization of soil Actinobacteria that degrades polyesters
show Abstracthide Abstract
An aliphatic aromatic copolyester, poly(butylene adipate-co-terephthalate) (PBAT) is a chemically synthetic and biodegradable polymer that exhibits good mechanical properties, similar to those of low-density polyethylene (LDPE), and can be a replacement for LDPE as biodegradable mulch films in the agricultural field. Biodegradability of PBAT is stable at high temperature conditions such as compost environments compared to mild temperature conditions such as soil environments. Considering that PBAT could be used in biodegradable agricultural applications such as mulching film, it is important to investigate the biodegradation mechanism at mild temperatures. A PBAT-degrading bacterium isolated from a soil environment, designated as strain NKCM2511, was studied in detail. Genetic and biochemical analysis revealed that the strain belongs to the genus Rhodococcus in the phylum Actinobacteria.
Sample: PBAT-degrading_bacteria
SAMD00245770 • DRS159184 • All experiments • All runs
Library:
Name: 2511
Instrument: DNBSEQ-G400
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: The concentration of a genomic DNA solution was measured by Synergy H1 (Bio Tek) and QuantiFluor dsDNA System (Promega). A genomic DNA was fractionalized with 500 bp. The whole-genome sequence library was constructed using MGIEasy Universal DNA Library Prep Set according to the manual. The library of high-throughput sequencing platforms was prepared using MGIEasy DNA Adapters-96 (Plate) kit. The concentration of the library was measured by Qubit and Qubit dsDNA HS assay kit. The quality of the library was confirmed by Fragment Analyzer and dsDNA 915 Reagent Kit (Advanced Analytical Technologies). Circularize DNA was prepared using MGI Circularization Kit according to the manual. DNA nano ball (DNB) was made using MGISEQ-2000RS High-throughput Sequencing Set. DNB (2x150 bp) was sequenced using DNBSEQ-G400.
Spot descriptor:
forward151  reverse

Runs: 1 run, 9.9M spots, 3G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
DRR2440849,920,5533G2.1Gb2020-10-14

ID:
12123856

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