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SRX1064225: Parus Major Bisulfite Seq blood data
1 ILLUMINA (Illumina HiSeq 2500) run: 292.4M spots, 59.1G bases, 38.8Gb downloads

Design: Blood and brain DNA libraries were constructed according to the Epitect whole genome bisulfite sequencing workflow (Illumina) with 18 PCR cycles. Whole-genome sequencing data were generated using the Illumina HiSeq 2500 platform at Business Unit Bioscience, Wageningen UR. The number of paired-end reads (101bp) were 358M and 292M for brain and blood, respectively. Raw sequencing reads were trimmed for quality (≥ 20) and adaptor sequence using trim_galore v.0.1.4
Submitted by: Netherlands Institute of Ecology
Study: Parus major Genome sequencing and assembly
show Abstracthide Abstract
Seasonal timing is a key life-history trait with major fitness consequences. The small songbird Parus major (great tit) for decades has been a model to study fitness traits like e.g. avian timing of reproduction. The research is closely linked to the impact of global climate change on timing and its consequences. Linking quantitative genetic variation in life-history traits with polymorphisms in specific genes is essential for better understanding the causes and consequences of the diversity in these traits. Genetic variation in life-history traits in wild songbirds has been demonstrated in many, often long-term, studies throughout the world. Linking this variation to genomic information requires the development of the necessary genomics tools specifically aimed at these non-model species. The assembly and annotation of the genome of the great tit will greatly enhance the further use of the great tit as a model species in this research field.
Sample: Bisufite converted reads from Blood
SAMN03781031 • SRS964345 • All experiments • All runs
Organism: Parus major
Library:
Instrument: Illumina HiSeq 2500
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Spot descriptor:
forward102  reverse

Pipeline: show...hide...
ProgramVersion
trim_galorev0.1.4
BSseeker2v2.0.6
Runs: 1 run, 292.4M spots, 59.1G bases, 38.8Gb
Run# of Spots# of BasesSizePublished
SRR2070791292,381,01859.1G38.8Gb2016-01-25

ID:
1543523

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