CDC25B cell division cycle 25B [Homo sapiens (human)] - Gene

Summary

Official Symbol: CDC25Bprovided by HGNC
Official Full Name: cell division cycle 25Bprovided by HGNC
Primary source: HGNC:HGNC:1726
See related: Ensembl:ENSG00000101224 MIM:116949; AllianceGenome:HGNC:1726
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary: CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in lymph node (RPKM 21.4), appendix (RPKM 15.3) and 24 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 20p13

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	20	NC_000020.11 (3786951..3806115)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	20	NC_060944.1 (3817891..3837037)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	20	NC_000020.10 (3767598..3786762)

Chromosome 20 - NC_000020.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcription factor E2F3 activates CDC25B to regulate DNA damage and promote mitoxantrone resistance in stomach adenocarcinoma.
Title: Transcription factor E2F3 activates CDC25B to regulate DNA damage and promote mitoxantrone resistance in stomach adenocarcinoma.
Exploring the mechanism of C473D mutation on CDC25B causing weak binding affinity with CDK2/CyclinA by molecular dynamics study.
Title: Exploring the mechanism of C473D mutation on CDC25B causing weak binding affinity with CDK2/CyclinA by molecular dynamics study.
Abeta monomers protect lens epithelial cells against oxidative stress by upregulating CDC25B.
Title: Aβ monomers protect lens epithelial cells against oxidative stress by upregulating CDC25B.
CDC25B induces cellular senescence and correlates with tumor suppression in a p53-dependent manner.
Title: CDC25B induces cellular senescence and correlates with tumor suppression in a p53-dependent manner.
Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B.
Title: Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B.
CDC25B is associated with the risk of hepatocellular carcinoma, but not related to persistent infection of hepatitis B virus in a Chinese population.
Title: CDC25B is associated with the risk of hepatocellular carcinoma, but not related to persistent infection of hepatitis B virus in a Chinese population.
Pharmacophore-guided discovery of CDC25 inhibitors causing cell cycle arrest and tumor regression.
Title: Pharmacophore-guided discovery of CDC25 inhibitors causing cell cycle arrest and tumor regression.
Increased expression of CDC25B or CDC25C are mechanisms by which skin cancers evade apoptotic cell death.
Title: CDC25B and CDC25C overexpression in nonmelanoma skin cancer suppresses cell death.
Depletion of TIAR accelerates mitotic entry and leads to chromosomal instability in response to replication stress, in a manner that can be alleviated by the concomitant depletion of Cdc25B or inhibition of CDK1. Since TIAR retains CDK1 in GMGs and attenuates CDK1 activity, we propose that the assembly of GMGs may represent a so far unrecognized mechanism that contributes to the activation of the G2/M checkpoint
Title: TIAR marks nuclear G2/M transition granules and restricts CDK1 activity under replication stress.
These findings indicate that CDC25B facilitates the dephosphorylation of nucleoprotein (NP), which is vital for regulating NP functions and the life cycle of influenza a virus.
Title: CDC25B promotes influenza A virus replication by regulating the phosphorylation of nucleoprotein.

Submit: New GeneRIF Correction See all GeneRIFs (74)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls. EBI GWAS Catalog EBI GWAS CatalogPubMed
Propensity score-based nonparametric test revealing genetic variants underlying bipolar disorder. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vpr	vpr	Kinase Srk1 is required for the Vpr-mediated nuclear export of Cdc25	PubMed
	vpr	HIV-1 Vpr induces subcellular relocalization of Cdc25 from nuclear to cytoplasm and Vpr-induced nuclear exclusion of Cdc25 depends on the serine/threonine phosphorylation of Cdc25 and the presence of Rad24/14-3-3 protein	PubMed

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D20S1012 (e-PCR)
- UniSTS:32857

SHGC-30160 (e-PCR)
- UniSTS:79344

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables histone H2AXY142 phosphatase activity	IEA Inferred from Electronic Annotation more info
enables non-membrane spanning protein tyrosine phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphoprotein phosphatase activity	TAS Traceable Author Statement more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine phosphatase activity	TAS Traceable Author Statement more info	PubMed
enables protein tyrosine phosphatase activity, metal-dependent	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in G2/M transition of mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in female meiosis I	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in oocyte maturation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of G2/M transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of G2/M transition of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of G2/MI transition of meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cell population proliferation	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: M-phase inducer phosphatase 2

Names: CDC25 homolog B; dual specificity phosphatase Cdc25B

NP_001274445.1

EC 3.1.3.48

NP_001274446.1

EC 3.1.3.48

NP_001274447.1

EC 3.1.3.48

NP_001274448.1

EC 3.1.3.48

NP_001274449.1

EC 3.1.3.48

NP_001274451.1

EC 3.1.3.48

NP_001274453.1

EC 3.1.3.48

NP_004349.1

EC 3.1.3.48

NP_068658.1

EC 3.1.3.48

NP_068659.1

EC 3.1.3.48

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029040.2 RefSeqGene

Range

14539..24344

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001287516.2 → NP_001274445.1 M-phase inducer phosphatase 2 isoform 4

See identical proteins and their annotated locations for NP_001274445.1

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at an upstream start codon, compared to variant 1. The resulting protein (isoform 4) has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AK295573, AL109804, DA107932, DB477384

Consensus CDS

CCDS74700.1

UniProtKB/TrEMBL

B4DIG0, B4DQZ3, E9PCK4

Related

ENSP00000339125.6, ENST00000344256.10

Conserved Domains (2) summary

cd01530
Location:348 → 467

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:49 → 319

M-inducer_phosp; M-phase inducer phosphatase
NM_001287517.2 → NP_001274446.1 M-phase inducer phosphatase 2 isoform 5

See identical proteins and their annotated locations for NP_001274446.1

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR which results in initiation of translation at an upstream start codon, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 5) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

AK299192, AL109804, DA107932, DB477384

UniProtKB/TrEMBL

B4DQZ3, B4DRC3

Conserved Domains (2) summary

cd01530
Location:334 → 453

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:35 → 305

M-inducer_phosp; M-phase inducer phosphatase
NM_001287518.2 → NP_001274447.1 M-phase inducer phosphatase 2 isoform 6

Status: REVIEWED

Description

Transcript Variant: This variant (6) differs in the 5' UTR, initiates translation at an upstream start codon, and lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 6) has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AK299028, AL109804, DA107932, DB477384

Consensus CDS

CCDS74701.1

UniProtKB/TrEMBL

B4DQZ3, G8JLH2

Related

ENSP00000368918.5, ENST00000379598.9

Conserved Domains (2) summary

cd01530
Location:321 → 440

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:49 → 292

M-inducer_phosp; M-phase inducer phosphatase
NM_001287519.2 → NP_001274448.1 M-phase inducer phosphatase 2 isoform 7

See identical proteins and their annotated locations for NP_001274448.1

Status: REVIEWED

Description

Transcript Variant: This variant (7) uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 7) has a shorter N-terminus compared to isoform 1. Variants 7 and 8 encode the same isoform.

Source sequence(s)

AL561439, BC051711, BM793120, BX647988

UniProtKB/TrEMBL

B3KS38, Q6MZW8

Conserved Domains (2) summary

cd01530
Location:300 → 419

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:1 → 271

M-inducer_phosp; M-phase inducer phosphatase
NM_001287520.2 → NP_001274449.1 M-phase inducer phosphatase 2 isoform 7

See identical proteins and their annotated locations for NP_001274449.1

Status: REVIEWED

Description

Transcript Variant: This variant (8), also known as CDC25B6, uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 7) has a shorter N-terminus compared to isoform 1. Variants 7 and 8 encode the same isoform.

Source sequence(s)

DA107932, FR695900

UniProtKB/TrEMBL

B3KS38, Q6MZW8

Conserved Domains (2) summary

cd01530
Location:300 → 419

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:1 → 271

M-inducer_phosp; M-phase inducer phosphatase
NM_001287522.2 → NP_001274451.1 M-phase inducer phosphatase 2 isoform 8

See identical proteins and their annotated locations for NP_001274451.1

Status: REVIEWED

Description

Transcript Variant: This variant (9) uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 8) has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AL561439, FR695901

UniProtKB/TrEMBL

E1YMX4, Q6MZW8

Conserved Domains (2) summary

cd01530
Location:259 → 378

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:1 → 230

M-inducer_phosp; M-phase inducer phosphatase
NM_001287524.2 → NP_001274453.1 M-phase inducer phosphatase 2 isoform 9

Status: REVIEWED

Description

Transcript Variant: This variant (10) differs in the 5' UTR and has multiple coding region differences, compared to variant 1, which causes translation initiation at a downstream start codon. The resulting protein (isoform 9) has a shorter N-terminus and lacks an internal region compared to isoform 1.

Source sequence(s)

AL109804, BC051711, BX640836, DA065196, DA107932

UniProtKB/TrEMBL

Q6MZW8

Conserved Domains (2) summary

cd01530
Location:220 → 339

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:1 → 191

M-inducer_phosp; M-phase inducer phosphatase
NM_004358.5 → NP_004349.1 M-phase inducer phosphatase 2 isoform 2

See identical proteins and their annotated locations for NP_004349.1

Status: REVIEWED

Description

Transcript Variant: This variant (2), also known as CDC25B1, uses an alternate splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1.

Source sequence(s)

BC051711, BX640836, S78187

Consensus CDS

CCDS13065.1

UniProtKB/TrEMBL

B4DQZ3

Related

ENSP00000405972.2, ENST00000439880.6

Conserved Domains (2) summary

cd01530
Location:398 → 517

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:99 → 369

M-inducer_phosp; M-phase inducer phosphatase
NM_021872.4 → NP_068658.1 M-phase inducer phosphatase 2 isoform 3

See identical proteins and their annotated locations for NP_068658.1

Status: REVIEWED

Description

Transcript Variant: This variant (3), also known as CDC25B2, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3) compared to isoform 1.

Source sequence(s)

BC051711, BX640836, S78187, Z68092

Consensus CDS

CCDS13066.1

UniProtKB/TrEMBL

B4DQZ3

Related

ENSP00000339170.4, ENST00000340833.4

Conserved Domains (2) summary

cd01530
Location:371 → 490

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:113 → 342

M-inducer_phosp; M-phase inducer phosphatase
NM_021873.4 → NP_068659.1 M-phase inducer phosphatase 2 isoform 1

See identical proteins and their annotated locations for NP_068659.1

Status: REVIEWED

Description

Transcript Variant: This variant (1), also known as CDC25B3, represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

BC051711, BX640836, S78187

Consensus CDS

CCDS13067.1

UniProtKB/Swiss-Prot

D3DVY1, D3DVY2, D3DVY3, D3DVY4, O43551, P30305, Q13971, Q5JX77, Q6RSS1, Q9BRA6

UniProtKB/TrEMBL

B4DQZ3

Related

ENSP00000245960.5, ENST00000245960.10

Conserved Domains (2) summary

cd01530
Location:412 → 531

Cdc25; Cdc25 phosphatases are members of the Rhodanese Homology Domain superfamily. They activate the cell division kinases throughout the cell cycle progression. Cdc25 phosphatases dephosphorylate phosphotyrosine and phosphothreonine residues, in order to ...

pfam06617
Location:113 → 383

M-inducer_phosp; M-phase inducer phosphatase

RNA

NR_136335.2 RNA Sequence

Status: REVIEWED

Source sequence(s)

AL109804, DA107932
NR_136336.2 RNA Sequence

Status: REVIEWED

Source sequence(s)

AL109804, DA107932, DB477384

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000020.11 Reference GRCh38.p14 Primary Assembly

Range

3786951..3806115

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

NC_060944.1 Alternate T2T-CHM13v2.0

Range

3817891..3837037

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_021874.2: Suppressed sequence

Description

NM_021874.2: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
NM_212530.1: Suppressed sequence

Description

NM_212530.1: This RefSeq was permanently suppressed because currently there is support for the transcript but not for the protein.