SLD2 Sld2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SLD2
Official Full Name: Sld2p
Primary source: SGD:S000001591
Locus tag: YKL108W
See related: AllianceGenome:SGD:S000001591; FungiDB:YKL108W; VEuPathDB:YKL108W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DRC1
Summary: Enables DNA replication origin binding activity and single-stranded DNA binding activity. Involved in DNA metabolic process; mitotic DNA replication checkpoint signaling; and negative regulation of protein-containing complex assembly. Located in cytoplasm and nucleus. Part of DNA replication preinitiation complex. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SLD2 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (234426..235787)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Repression of CDC6 and SLD2 during meiosis coincides with expression of shortened isoforms.
Title: Transcriptional repression of CDC6 and SLD2 during meiosis is associated with production of short heterogeneous RNA isoforms.
Dma1/2 mediate rapid Sld2 degradation by recognizing a complex phospho-degron
Title: Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2.
These data suggest that Sld2 association with DNA is required for CMG assembly in S phase.
Title: The replication initiation protein Sld2 regulates helicase assembly.
origin ssDNA may stimulate the formation of the CMG complex by alleviating inhibitory interactions between Sld2 with Mcm2-7.
Title: Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA.
CDK promotes the formation of a newly identified fragile complex, the preloading complex (pre-LC) containing DNA polymerase epsilon (Pol epsilon), GINS, Sld2, and Dpb11
Title: CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast.
MSA1 is a high copy suppressor of a drc1-1 mutant.
Title: Identification of MSA1, a cell cycle-regulated, dosage suppressor of drc1/sld2 and dpb11 mutants.
Sld2 and Sld3 are the minimal set of cyclin-dependent kinase targets required for DNA replication
Title: Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast.
Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS
Title: CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.
Phosphorylation of canonical motifs in Sld2 does not play a direct role in complex formation with Dpb11, but rather regulates phosphorylation of another residue, Thr84.
Title: A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2-Dpb11.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA replication origin binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA replication preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication preinitiation complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of DNA replication preinitiation complex	IGI Inferred from Genetic Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Sld2p

NP_012814.1

Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

234426..235787

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179674.1 → NP_012814.1 TPA: Sld2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012814.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXH9, P34252

UniProtKB/TrEMBL

A6ZZK8, G2WHS5, N1P046

Conserved Domains (1) summary

cl26235
Location:9 → 453

RecQL4_SLD2_NTD; N-terminal homeodomain-like domain of metazoan RecQ protein-like 4 (RecQL4), fungal DNA replication regulator SLD2 and similar proteins