Actn1 actinin, alpha 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Actn1provided by RGD
Official Full Name: actinin, alpha 1provided by RGD
Primary source: RGD:70907
See related: EnsemblRapid:ENSRNOG00000056756 AllianceGenome:RGD:70907
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables cytoskeletal regulatory protein binding activity and protein domain specific binding activity. Predicted to be involved in several processes, including actin cytoskeleton organization; focal adhesion assembly; and platelet formation. Located in actin cytoskeleton; dendritic spine; and plasma membrane. Is active in postsynaptic actin cytoskeleton. Human ortholog(s) of this gene implicated in platelet-type bleeding disorder 15. Orthologous to human ACTN1 (actinin alpha 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Spleen (RPKM 260.1), Uterus (RPKM 235.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 6q24

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	6	NC_086024.1 (104731485..104826312, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	6	NC_051341.1 (98998553..99093334, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	6	NC_005105.4 (103376557..103470497, complement)

Chromosome 6 - NC_086024.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

that a mechanism by which Hippo pathway and F-actin synergize to modulate YAP activation and localization in the context of intervertebral disc degeneration and help to control nucleus pulposus cells proliferation
Title: AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration.
After 6 hours of antiorthostatic suspension alpha-actinin 1 and alpha-actinin 4 levels decreased in the membranous fraction of proteins of cardiomyocytes and soleus muscle fibers.
Title: Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.
Desmin and alpha-actinin-2 content in rat soleus muscle in the dynamics of gravitational unloading and subsequent reloading.
Title: Desmin and α-actinin-2 content in rat soleus muscle in the dynamics of gravitational unloading and subsequent reloading.
The alpha-actinin-1 form a complex with CLP36 and RIL.
Title: CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.
Data describe alterations of myocardial intercellular and cell-matrix contacts in hypertrophic tissue, and show intracellular translocation of beta-catenin, alpha-actinin and chondroitin sulfate proteoglycan 6 in both an animal model and in LVH patients.
Title: Expression and tissue localization of beta-catenin, alpha-actinin and chondroitin sulfate proteoglycan 6 is modulated during rat and human left ventricular hypertrophy.
Alpha-Actinin mediates the formation of the template and organization of adhesions associated with actin filaments, suggesting that actin crosslinking has a major role in this process.
Title: Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner.
two sm-titin Zq domains interact with each other and with the two R2-R3 sites in the alpha-actinin central rod
Title: Smooth muscle titin Zq domain interaction with the smooth muscle alpha-actinin central rod.
Cardiac dysfunction induced by peroxynitrite involves degradation of alpha-actinin that may be mediated by the proteolytic action of MMP-2.
Title: Matrix metalloproteinase-2 degrades the cytoskeletal protein alpha-actinin in peroxynitrite mediated myocardial injury.
PINCH1, Ilk and actinin from a complex to costameres in neonatal rat ventricular myocytes. This complex is stimulated by fibronectins and phenylephrine, and by both by drug synergism, to regulate cardiac myocyte hypertrophy.
Title: Role of the integrin-linked kinase/PINCH1/alpha-parvin complex in cardiac myocyte hypertrophy.
an alpha-actinin-dependent association between the actin cytoskeleton and A2AR trafficking
Title: The adenosine A2A receptor interacts with the actin-binding protein alpha-actinin.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH142024 (e-PCR)
- UniSTS:224015

BE098731 (e-PCR)
- UniSTS:245290

BE119221 (e-PCR)
- UniSTS:249133

C77473 (e-PCR)
- UniSTS:208829

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	ISO Inferred from Sequence Orthology more info
enables alpha-actinin binding	ISS Inferred from Sequence or Structural Similarity more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal regulatory protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables double-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables integrin binding	IEA Inferred from Electronic Annotation more info
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables nuclear receptor coactivator activity	IEA Inferred from Electronic Annotation more info
enables nuclear receptor coactivator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables structural constituent of postsynapse	IEA Inferred from Electronic Annotation more info
enables structural constituent of postsynapse	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	IEA Inferred from Electronic Annotation more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info
enables vinculin binding	ISO Inferred from Sequence Orthology more info
enables vinculin binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament bundle assembly	IEA Inferred from Electronic Annotation more info
involved_in actin filament bundle assembly	ISO Inferred from Sequence Orthology more info
involved_in actin filament network formation	IEA Inferred from Electronic Annotation more info
involved_in actin filament network formation	ISO Inferred from Sequence Orthology more info
involved_in actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in focal adhesion assembly	IEA Inferred from Electronic Annotation more info
involved_in focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in muscle cell development	IBA Inferred from Biological aspect of Ancestor more info
NOT involved_in platelet activation	ISO Inferred from Sequence Orthology more info
NOT involved_in platelet aggregation	ISO Inferred from Sequence Orthology more info
involved_in platelet formation	IEA Inferred from Electronic Annotation more info
involved_in platelet formation	ISO Inferred from Sequence Orthology more info
involved_in platelet morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in postsynapse organization	IEA Inferred from Electronic Annotation more info
involved_in sarcomere organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in skeletal muscle fiber development	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
is_active_in Z disc	IBA Inferred from Biological aspect of Ancestor more info
located_in Z disc	IEA Inferred from Electronic Annotation more info
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in Z disc	ISS Inferred from Sequence or Structural Similarity more info
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in actin filament	IEA Inferred from Electronic Annotation more info
located_in actin filament	ISO Inferred from Sequence Orthology more info
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in bicellular tight junction	ISS Inferred from Sequence or Structural Similarity more info
located_in brush border	IEA Inferred from Electronic Annotation more info
located_in brush border	ISO Inferred from Sequence Orthology more info
is_active_in cell junction	IBA Inferred from Biological aspect of Ancestor more info
located_in cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell leading edge	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cell projection	IBA Inferred from Biological aspect of Ancestor more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
is_active_in cortical actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cortical actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in dense body	ISS Inferred from Sequence or Structural Similarity more info
located_in fascia adherens	IEA Inferred from Electronic Annotation more info
located_in fascia adherens	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISS Inferred from Sequence or Structural Similarity more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in inner dense plaque of desmosome	ISS Inferred from Sequence or Structural Similarity more info
located_in lamellipodium	ISS Inferred from Sequence or Structural Similarity more info
located_in lateral plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in outer dense plaque of desmosome	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
located_in ruffle	IEA Inferred from Electronic Annotation more info
located_in ruffle	ISO Inferred from Sequence Orthology more info
located_in ruffle	ISS Inferred from Sequence or Structural Similarity more info
located_in sarcolemma	ISS Inferred from Sequence or Structural Similarity more info
located_in sarcomere	ISO Inferred from Sequence Orthology more info
located_in smooth muscle dense body	ISS Inferred from Sequence or Structural Similarity more info
is_active_in stress fiber	IBA Inferred from Biological aspect of Ancestor more info
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed
located_in stress fiber	ISO Inferred from Sequence Orthology more info
located_in stress fiber	ISS Inferred from Sequence or Structural Similarity more info
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in terminal web	ISS Inferred from Sequence or Structural Similarity more info
located_in zonula adherens	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: alpha-actinin-1

Names: F-actin cross-linking protein; non-muscle alpha-actinin-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031005.4 → NP_112267.1 alpha-actinin-1

See identical proteins and their annotated locations for NP_112267.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000006

UniProtKB/Swiss-Prot

Q9Z1P2

UniProtKB/TrEMBL

A0A8I6AV34

Related

ENSRNOP00000068851.2, ENSRNOT00000091560.3

Conserved Domains (6) summary

cd00051
Location:750 → 817

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd00176
Location:395 → 622

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

pfam00435
Location:634 → 733

Spectrin; Spectrin repeat

pfam08726
Location:822 → 888

EFhand_Ca_insen; Ca2+ insensitive EF hand

cd21214
Location:28 → 132

CH_ACTN_rpt1; first calponin homology (CH) domain found in the alpha-actinin family

cd21216
Location:136 → 250

CH_ACTN_rpt2; second calponin homology (CH) domain found in the alpha-actinin family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086024.1 Reference GRCr8

Range

104731485..104826312 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039112993.2 → XP_038968921.1 alpha-actinin-1 isoform X2

UniProtKB/TrEMBL

A0A8I6AV34, Q6GMN8

Conserved Domains (6) summary

COG5126
Location:743 → 847

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

cd00176
Location:395 → 622

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

pfam00435
Location:634 → 733

Spectrin; Spectrin repeat

pfam08726
Location:817 → 883

EFhand_Ca_insen; Ca2+ insensitive EF hand

cd21214
Location:28 → 132

CH_ACTN_rpt1; first calponin homology (CH) domain found in the alpha-actinin family

cd21216
Location:136 → 250

CH_ACTN_rpt2; second calponin homology (CH) domain found in the alpha-actinin family
XM_039112992.2 → XP_038968920.1 alpha-actinin-1 isoform X1

UniProtKB/TrEMBL

A0A8I6AV34, Q6T487

Related

ENSRNOP00000074946.2, ENSRNOT00000079824.3

Conserved Domains (6) summary

cd00051
Location:750 → 839

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

cd00176
Location:395 → 622

SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...

pfam00435
Location:634 → 733

Spectrin; Spectrin repeat

pfam08726
Location:844 → 910

EFhand_Ca_insen; Ca2+ insensitive EF hand

cd21214
Location:28 → 132

CH_ACTN_rpt1; first calponin homology (CH) domain found in the alpha-actinin family

cd21216
Location:136 → 250

CH_ACTN_rpt2; second calponin homology (CH) domain found in the alpha-actinin family
XM_063262549.1 → XP_063118619.1 alpha-actinin-1 isoform X4
XM_063262548.1 → XP_063118618.1 alpha-actinin-1 isoform X3

Related

ENSRNOP00000085943.1, ENSRNOT00000112260.2