Dapk3 death-associated protein kinase 3 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dapk3provided by RGD
Official Full Name: death-associated protein kinase 3provided by RGD
Primary source: RGD:621766
See related: EnsemblRapid:ENSRNOG00000020383 AllianceGenome:RGD:621766
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Dapkl
Summary: Enables ATP binding activity; protein C-terminus binding activity; and protein serine/threonine kinase activity. Involved in several processes, including intracellular signal transduction; neuron differentiation; and positive regulation of extrinsic apoptotic signaling pathway in absence of ligand. Located in several cellular components, including actin filament; membrane raft; and nucleus. Orthologous to human DAPK3 (death associated protein kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Heart (RPKM 100.4), Adrenal (RPKM 94.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q11

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (9174903..9183272, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (8524182..8532552, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (11392436..11400855, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Death-associated protein kinase 3 regulates the myogenic reactivity of cerebral arteries.
Title: Death-associated protein kinase 3 regulates the myogenic reactivity of cerebral arteries.
DAPK3 modulated VSMC calcification through AMPK-mediated ERS signaling
Title: Death-associated protein kinase 3 deficiency alleviates vascular calcification via AMPK-mediated inhibition of endoplasmic reticulum stress.
Estrogen promotes ethanol-evoked myocardial oxidative stress and left ventricular dysfunction via DAPK3/Akt/ERK activation in male rats.
Title: Estrogen modulation of the ethanol-evoked myocardial oxidative stress and dysfunction via DAPK3/Akt/ERK activation in male rats.
Data suggest that intestinal edema inhibits MLC phosphorylation by decreasing inhibitory phosphorylation of MYPT1 via decreased ROCK and ZIPK activities, resulting in more MLC phosphatase activity.
Title: Decreased myosin phosphatase target subunit 1(MYPT1) phosphorylation via attenuated rho kinase and zipper-interacting kinase activities in edematous intestinal smooth muscle.
ZIPK is involved in the regulation of smooth muscle contraction through direct phosphorylation of LC(20).
Title: Chemical genetics of zipper-interacting protein kinase reveal myosin light chain as a bona fide substrate in permeabilized arterial smooth muscle.
ZIPK may act as a cardiac RLC kinase and thereby affect contractility.
Title: Cardiac myosin is a substrate for zipper-interacting protein kinase (ZIPK).
Results suggest that CPI-17 and ZIPK participate in the regulation of PKC-alpha and PKC-epsilon on vascular calcium sensitivity after hemorrhagic shock.
Title: Involvement of Cpi-17 and zipper-interacting protein kinase in the regulation of protein kinase C-alpha, protein kinase C-epsilon on vascular calcium sensitivity after hemorrhagic shock.
Par-4 facilitates contraction by targeting ZIPK to the vicinity of its substrates, myosin light chain and MYPT, which are located on the actin filaments.
Title: The pro-apoptotic protein Par-4 facilitates vascular contractility by cytoskeletal targeting of ZIPK.
Ca2+-independent contraction of longitudinal ileal smooth muscle is potentiated by a zipper-interacting protein kinase pseudosubstrate peptide.
Title: Ca2+-independent contraction of longitudinal ileal smooth muscle is potentiated by a zipper-interacting protein kinase pseudosubstrate peptide.
results demonstrate that DAPK is a S6 kinase and provide evidence for a novel role of DAPK in the regulation of translation
Title: Death-associated protein kinase phosphorylates mammalian ribosomal protein S6 and reduces protein synthesis.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH139169 (e-PCR)
- UniSTS:221255

RH127807 (e-PCR)
- UniSTS:211117

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables leucine zipper domain binding	IEA Inferred from Electronic Annotation more info
enables leucine zipper domain binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cellular response to type II interferon	IEA Inferred from Electronic Annotation more info
involved_in cellular response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in neuron differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of extrinsic apoptotic signaling pathway in absence of ligand	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autophosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell shape	ISO Inferred from Sequence Orthology more info
involved_in regulation of focal adhesion assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of myosin II filament organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of myosin II filament organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in PML body	IEA Inferred from Electronic Annotation more info
located_in PML body	ISO Inferred from Sequence Orthology more info
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule organizing center	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: death-associated protein kinase 3

Names: DAP kinase 3; DAP-like kinase; Death-associated like kinase; MYPT1 kinase; ZIP-kinase; dlk

NP_071991.1

EC 2.7.11.1

XP_006241053.1

EC 2.7.11.1

XP_006241055.1

EC 2.7.11.1

XP_017450588.1

EC 2.7.11.1

XP_038935784.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_022546.2 → NP_071991.1 death-associated protein kinase 3

See identical proteins and their annotated locations for NP_071991.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

O88764

UniProtKB/TrEMBL

A0A8I6GL62, A6K8B5

Related

ENSRNOP00000027634.5, ENSRNOT00000027634.6

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

9174903..9183272 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006240991.3 → XP_006241053.1 death-associated protein kinase 3 isoform X1

UniProtKB/TrEMBL

A0A8I6GL62

Related

ENSRNOP00000081984.2, ENSRNOT00000096134.2

Conserved Domains (2) summary

smart00220
Location:29 → 291

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14105
Location:23 → 291

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
XM_017595099.3 → XP_017450588.1 death-associated protein kinase 3 isoform X2

UniProtKB/Swiss-Prot

O88764

UniProtKB/TrEMBL

A0A8I6GL62, A6K8B5

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
XM_006240993.4 → XP_006241055.1 death-associated protein kinase 3 isoform X2

See identical proteins and their annotated locations for XP_006241055.1

UniProtKB/Swiss-Prot

O88764

UniProtKB/TrEMBL

A0A8I6GL62, A6K8B5

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase
XM_039079856.2 → XP_038935784.1 death-associated protein kinase 3 isoform X2

UniProtKB/Swiss-Prot

O88764

UniProtKB/TrEMBL

A0A8I6GL62, A6K8B5

Conserved Domains (1) summary

cd14105
Location:7 → 275

STKc_DAPK; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase