Dynll1 dynein light chain LC8-type 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dynll1provided by RGD
Official Full Name: dynein light chain LC8-type 1provided by RGD
Primary source: RGD:619866
See related: EnsemblRapid:ENSRNOG00000083010 AllianceGenome:RGD:619866
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pin; Dlc8; 8kDLC; Dnclc1
Summary: Enables several functions, including identical protein binding activity; nitric-oxide synthase inhibitor activity; and scaffold protein binding activity. Involved in positive regulation of insulin secretion involved in cellular response to glucose stimulus and spermatid development. Acts upstream of or within negative regulation of nitric oxide biosynthetic process. Located in axon cytoplasm and secretory granule. Part of cytoplasmic dynein complex. Used to study hypertension and impotence. Biomarker of cardiac arrest; congestive heart failure; and middle cerebral artery infarction. Orthologous to human DYNLL1 (dynein light chain LC8-type 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 1546.2), Lung (RPKM 1162.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12q16

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (46945291..46976867)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (41312365..41316157)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (47074200..47076573)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

It has been found that APC2 localizes as distinct clusters along microtubule bundles in dendrites, and that this localization is driven by LC8-binding and two separate microtubule-interacting domains.
Title: APC2 controls dendrite development by promoting microtubule dynamics.
silencing of Dynein light chain LC8 or class I transcription factor A2 resulted in a loss of CITFA from RNA Pol I promoters
Title: Dynein Light Chain LC8 Is Required for RNA Polymerase I-Mediated Transcription in Trypanosoma brucei, Facilitating Assembly and Promoter Binding of Class I Transcription Factor A.
Data suggest that redox-sensitive and protein inhibitor of nNOS (PIN)-mediated neuronal NO synthase (nNOS) uncoupling is engaged in a vicious cycle that sustains the production of reactive oxygen species in rostral ventrolateral medulla (RVLM).
Title: Role of nitric oxide synthase uncoupling at rostral ventrolateral medulla in redox-sensitive hypertension associated with metabolic syndrome.
results suggest that PIN is involved in the development of hypertension.
Title: RNA silencing targeting PIN (protein inhibitor of neuronal nitric oxide synthase) attenuates the development of hypertension in young spontaneously hypertensive rats.
Our results show a novel intermediary mechanism that leads to decreased levels of active nNOS in the PVN, involved in subsequent reduction in sympathoinhibition during CHF, offering a new target for the treatment of CHF and other cardiovascular diseases.
Title: Angiotensin II-mediated posttranslational modification of nNOS in the PVN of rats with CHF: role for PIN.
In the glaucoma model DYNLL1 was strongly and persistently downregulated at all timepoints
Title: The expression of dynein light chain DYNLL1 (LC8-1) is persistently downregulated in glaucomatous rat retinal ganglion cells.
Our studies suggest an unexpected role for LC8 and provide new mechanistic insights into how SNPH and LC8 together immobilize mitochondria through a dynamic interaction between the docking receptor and axonal cytoskeleton.
Title: Dynein light chain LC8 regulates syntaphilin-mediated mitochondrial docking in axons.
Data show that Bassoon functions as a cargo adapter for retrograde transport and that disruption of the Bassoon-dynein light chain 1/2 interactions leads to impaired trafficking of Bassoon and affects distribution of Bassoon and Piccolo among synapses.
Title: Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon.
Peptide fragments synthesized to determine binding sites of other proteins.
Title: Cellulose membrane supported peptide arrays for deciphering protein-protein interaction sites: the case of PIN, a protein with multiple natural partners.
the backbone dynamics of DLC8, both in its apo- and target-peptide bound forms, were characterized by 15N NMR relaxation studies
Title: Backbone dynamics of the 8 kDa dynein light chain dimer reveals molecular basis of the protein's functional diversity.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BF418128 (e-PCR)
- UniSTS:249911

Dynll1 (e-PCR), detects polymorphism
- UniSTS:472905

RH128914 (e-PCR)
- UniSTS:212222

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables deoxyribonuclease inhibitor activity	ISO Inferred from Sequence Orthology more info
enables dynein intermediate chain binding	IBA Inferred from Biological aspect of Ancestor more info
enables dynein intermediate chain binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme inhibitor activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables nitric-oxide synthase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables nitric-oxide synthase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in intraciliary retrograde transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intraciliary retrograde transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitocytosis	ISO Inferred from Sequence Orthology more info
involved_in motile cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within motile cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA strand resection involved in replication fork processing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA strand resection involved in replication fork processing	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion involved in cellular response to glucose stimulus	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of non-motile cilium assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of mitochondrial membrane potential	ISO Inferred from Sequence Orthology more info
involved_in spermatid development	IEP Inferred from Expression Pattern more info	PubMed
involved_in substantia nigra development	ISO Inferred from Sequence Orthology more info	PubMed

Component	Evidence Code	Pubs
part_of COP9 signalosome	ISO Inferred from Sequence Orthology more info
located_in axon cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cilium	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
part_of cytoplasmic dynein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cytoplasmic dynein complex	IDA Inferred from Direct Assay more info	PubMed
part_of cytoplasmic dynein complex	ISO Inferred from Sequence Orthology more info
part_of cytoplasmic dynein complex	ISS Inferred from Sequence or Structural Similarity more info
part_of cytoplasmic dynein complex	TAS Traceable Author Statement more info	PubMed
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
part_of dynein complex	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISO Inferred from Sequence Orthology more info
located_in kinetochore	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	ISO Inferred from Sequence Orthology more info
located_in mitotic spindle	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of double-strand break	ISO Inferred from Sequence Orthology more info
is_active_in site of double-strand break	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: dynein light chain 1, cytoplasmic

Names: 8 kDa dynein light chain; 8kD LC; dynein LC8; dynein, cytoplasmic, light chain 1; protein inhibitor of neuronal nitric oxide synthase

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053319.5 → NP_445771.1 dynein light chain 1, cytoplasmic

See identical proteins and their annotated locations for NP_445771.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000012

UniProtKB/Swiss-Prot

P63170

UniProtKB/TrEMBL

A6J1V5

Related

ENSRNOP00000106438.1, ENSRNOT00000165275.1

Conserved Domains (1) summary

cd21452
Location:5 → 88

DLC-like_DYNLL1_DYNLL2; dynein light chain (DLC)-like domain found in the cytoplasmic dynein light chain 1 (DYNLL1) family