Mettl3 methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mettl3provided by MGI
Official Full Name: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunitprovided by MGI
Primary source: MGI:MGI:1927165
See related: Ensembl:ENSMUSG00000022160 AllianceGenome:MGI:1927165
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: M6A; Spo8; 2310024F18Rik
Summary: Enables mRNA m(6)A methyltransferase activity. Involved in several processes, including RNA metabolic process; forebrain radial glial cell differentiation; and mRNA destabilization. Located in nucleus. Part of RNA N6-methyladenosine methyltransferase complex. Is expressed in brain; genitourinary system; hindlimb; intestine; and liver. Orthologous to human METTL3 (methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 8.6), CNS E14 (RPKM 7.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 C2; 14 26.88 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (52532298..52548555, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (52294841..52308741, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

METTL3-mediated m6A modification enhances lncRNA H19 stability to promote endothelial cell inflammation and pyroptosis to aggravate atherosclerosis.
Title: METTL3-mediated m6A modification enhances lncRNA H19 stability to promote endothelial cell inflammation and pyroptosis to aggravate atherosclerosis.
METTL3's role in cervical cancer development through m[6]A modification.
Title: METTL3's role in cervical cancer development through m(6)A modification.
Mettl3-m6A-YTHDF1 axis promotion of mitochondrial dysfunction in metabolic dysfunction-associated steatotic liver disease.
Title: Mettl3-m6A-YTHDF1 axis promotion of mitochondrial dysfunction in metabolic dysfunction-associated steatotic liver disease.
METTL3 regulates cartilage development and homeostasis by affecting Lats1 mRNA stability in an m[6]A-YTHDF2-dependent manner.
Title: METTL3 regulates cartilage development and homeostasis by affecting Lats1 mRNA stability in an m(6)A-YTHDF2-dependent manner.
METTL3 deficiency leads to ovarian insufficiency due to IL-1beta overexpression in theca cells.
Title: METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells.
METTL3-mediated pre-miR-665/DLX3 m[6]A methylation facilitates the committed differentiation of stem cells from apical papilla.
Title: METTL3-mediated pre-miR-665/DLX3 m(6)A methylation facilitates the committed differentiation of stem cells from apical papilla.
Inhibition of Mettl3 ameliorates osteoblastic senescence by mitigating m6A modifications on Slc1a5 via Igf2bp2-dependent mechanisms.
Title: Inhibition of Mettl3 ameliorates osteoblastic senescence by mitigating m6A modifications on Slc1a5 via Igf2bp2-dependent mechanisms.
[Mechanisms by which Mettl3 regulates pericyte-myofibroblast transdifferentiation through PI3K/AKT signaling pathway].
Title: [Mechanisms by which Mettl3 regulates pericyte-myofibroblast transdifferentiation through PI3K/AKT signaling pathway].
METTL3-mediated NDUFB5 m6A modification promotes cell migration and mitochondrial respiration to promote the wound healing of diabetic foot ulcer.
Title: METTL3-mediated NDUFB5 m6A modification promotes cell migration and mitochondrial respiration to promote the wound healing of diabetic foot ulcer.
METTL3 regulates thyroid cancer differentiation and chemosensitivity by modulating PAX8.
Title: METTL3 regulates thyroid cancer differentiation and chemosensitivity by modulating PAX8.

Submit: New GeneRIF Correction See all GeneRIFs (172)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (14) 1 citation
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables S-adenosyl-L-methionine binding	ISO Inferred from Sequence Orthology more info
enables S-adenosyl-L-methionine binding	ISS Inferred from Sequence or Structural Similarity more info
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables mRNA m(6)A methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA m(6)A methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables mRNA m(6)A methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA m(6)A methyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables mRNA m(6)A methyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein heterodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein heterodimerization activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
involved_in DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
involved_in RNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within RNA methylation	ISO Inferred from Sequence Orthology more info	PubMed
involved_in RNA methylation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to UV	ISO Inferred from Sequence Orthology more info
involved_in cellular response to UV	ISS Inferred from Sequence or Structural Similarity more info
involved_in circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dosage compensation by inactivation of X chromosome	ISO Inferred from Sequence Orthology more info
involved_in dosage compensation by inactivation of X chromosome	ISS Inferred from Sequence or Structural Similarity more info
involved_in endothelial to hematopoietic transition	ISS Inferred from Sequence or Structural Similarity more info
involved_in forebrain radial glial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gliogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in innate immune response	IEA Inferred from Electronic Annotation more info
involved_in mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA modification	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA modification	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within mRNA modification	ISO Inferred from Sequence Orthology more info	PubMed
involved_in mRNA modification	ISO Inferred from Sequence Orthology more info
involved_in mRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	ISO Inferred from Sequence Orthology more info
NOT involved_in mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA splicing, via spliceosome	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of Notch signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of type I interferon-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of type I interferon-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cap-independent translational initiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cap-independent translational initiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mRNA catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in primary miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in primary miRNA processing	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of hematopoietic stem cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
part_of RNA N6-methyladenosine methyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA N6-methyladenosine methyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA N6-methyladenosine methyltransferase complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: N6-adenosine-methyltransferase subunit METTL3

Names: MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; m6a methyltransferase; methyltransferase like 3; methyltransferase-like protein 3

NP_062695.2

EC 2.1.1.348

XP_006519374.1

EC 2.1.1.348

XP_017171585.1

EC 2.1.1.348

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019721.2 → NP_062695.2 N6-adenosine-methyltransferase subunit METTL3

See identical proteins and their annotated locations for NP_062695.2

Status: VALIDATED

Source sequence(s)

AK085189, BE979768, BY108113

Consensus CDS

CCDS27053.1

UniProtKB/Swiss-Prot

Q8C3P7, Q9CV54, Q9ERS9, Q9WUI4

UniProtKB/TrEMBL

A0A0R4J041

Related

ENSMUSP00000022767.10, ENSMUST00000022767.16

Conserved Domains (2) summary

pfam05063
Location:389 → 550

MT-A70; MT-A70

pfam08619
Location:17 → 79

Nha1_C; Alkali metal cation/H+ antiporter Nha1 C terminus

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

52532298..52548555 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006519311.5 → XP_006519374.1 N6-adenosine-methyltransferase subunit METTL3 isoform X1

Conserved Domains (1) summary

pfam05063
Location:234 → 395

MT-A70
XM_017316096.3 → XP_017171585.1 N6-adenosine-methyltransferase subunit METTL3 isoform X1

Conserved Domains (1) summary

pfam05063
Location:234 → 395

MT-A70