U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

GNAI2 G protein subunit alpha i2 [ Homo sapiens (human) ]

Gene ID: 2771, updated on 10-Oct-2024

Summary

Official Symbol
GNAI2provided by HGNC
Official Full Name
G protein subunit alpha i2provided by HGNC
Primary source
HGNC:HGNC:4385
See related
Ensembl:ENSG00000114353 MIM:139360; AllianceGenome:HGNC:4385
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
GIP; HG1C; GNAI2B; H_LUCA15.1; H_LUCA16.1
Summary
The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Expression
Ubiquitous expression in spleen (RPKM 106.7), bone marrow (RPKM 93.2) and 25 other tissues See more
Orthologs
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

See GNAI2 in Genome Data Viewer
Location:
3p21.31
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (50227068..50263358)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (50256406..50288697)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (50264500..50296794)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50230799-50231315 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50231316-50231832 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50231833-50232348 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50232401-50232932 Neighboring gene G protein subunit alpha transducin 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14384 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50246621-50247149 Neighboring gene solute carrier family 38 member 3 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50250030-50250839 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_70487 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50257474-50257974 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50257975-50258475 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50263389-50264198 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14385 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19890 Neighboring gene microRNA 5787 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50272293-50272903 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50272904-50273514 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19891 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50275957-50276566 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14387 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19892 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50280231-50280841 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50280842-50281451 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50283071-50283786 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50283787-50284502 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14388 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50297648-50298153 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:50298661-50299165 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:50301209-50301784 Neighboring gene H3K27ac-H3K4me1 hESC enhancers GRCh37_chr3:50301785-50302358 and GRCh37_chr3:50302359-50302934 Neighboring gene SEMA3B antisense RNA 1 (head to head) Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19897 Neighboring gene semaphorin 3B Neighboring gene microRNA 6872

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120-mediated phosphorylation of WAVE2 requires both G(alpha)i-dependent and -independent pathways, and is conserved both in X4 and R5 viral infection of resting CD4 T cells and primary macrophages PubMed
env HIV-1 gp120 induces IL-1beta release from macrophages in a time- and concentration-dependent manner through binding to the chemokine receptor CCR5 and coupling to G(i)alpha protein PubMed
env CXCR4-tropic and CXCR4/CCR5 dual-tropic HIV-1 gp120 induce the cleavage of CD62 ligand by a mechanism dependent on matrix metalloproteinases 1 and 3, CD4, CXCR4, Galpha(i), and p38 MAPK, whereas CCR5-tropic gp120 does not PubMed
Nef nef HIV-1 Nef induced degradation of GNAI2 involves E3 ubiquitin ligases AIP4 and NEDD4 through recruitment of AIP4 and NEDD4 by Nef PubMed
nef HIV-1 Nef interacts with GNAI2 and induces lysosomal degradation of GNAI2 by ubiquitinylating GNAI2 at position Lys-296. The N-terminal myristoyl group, 62EEEE65 motif, and 72PXXP75 motif of Nef are critical for this effect to occur PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables G protein-coupled receptor binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables G-protein beta/gamma-subunit complex binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables GTP binding IEA
Inferred from Electronic Annotation
more info
 
enables GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
involved_in G protein-coupled acetylcholine receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
 
involved_in G protein-coupled adenosine receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in G protein-coupled receptor signaling pathway TAS
Traceable Author Statement
more info
PubMed 
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
 
involved_in adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in cell division IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in cell population proliferation IEA
Inferred from Electronic Annotation
more info
 
involved_in gamma-aminobutyric acid signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in negative regulation of adenylate cyclase activity TAS
Traceable Author Statement
more info
PubMed 
involved_in negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of apoptotic signaling pathway IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of calcium ion-dependent exocytosis IEA
Inferred from Electronic Annotation
more info
 
involved_in negative regulation of synaptic transmission IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of ERK1 and ERK2 cascade IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of cell migration IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of cell population proliferation IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in positive regulation of insulin receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of neural precursor cell proliferation IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of superoxide anion generation IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of urine volume IEA
Inferred from Electronic Annotation
more info
 
involved_in positive regulation of vascular associated smooth muscle cell proliferation IEA
Inferred from Electronic Annotation
more info
 
involved_in regulation of calcium ion transport IEA
Inferred from Electronic Annotation
more info
 
involved_in response to nutrient TAS
Traceable Author Statement
more info
PubMed 
involved_in signal transduction TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
located_in cell body IEA
Inferred from Electronic Annotation
more info
 
located_in centrosome IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 
located_in dendrite IEA
Inferred from Electronic Annotation
more info
 
located_in extracellular exosome HDA PubMed 
located_in extracellular vesicle HDA PubMed 
part_of heterotrimeric G-protein complex IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in hippocampal mossy fiber to CA3 synapse IEA
Inferred from Electronic Annotation
more info
 
located_in membrane HDA PubMed 
located_in midbody IDA
Inferred from Direct Assay
more info
PubMed 
located_in neuronal dense core vesicle IEA
Inferred from Electronic Annotation
more info
 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
located_in plasma membrane TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
guanine nucleotide-binding protein G(i) subunit alpha-2
Names
GTP-binding regulatory protein Gi alpha-2 chain
adenylate cyclase-inhibiting G alpha protein
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
guanine nucleotide-binding protein G(i), alpha-2 subunit
heterotrimeric guanine nucleotide-binding protein 1C

NCBI Reference Sequences (RefSeq)

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_016002.3 RefSeqGene

    Range
    14138..37296
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001166425.2NP_001159897.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2

    See identical proteins and their annotated locations for NP_001159897.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AK055574, AK126708, AK302330, DB044421, U73166
    Consensus CDS
    CCDS54587.1
    UniProtKB/TrEMBL
    B3KX51
    Related
    ENSP00000406369.1, ENST00000451956.1
    Conserved Domains (1) summary
    cd00066
    Location:3312
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
  2. NM_001282617.2NP_001269546.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3

    See identical proteins and their annotated locations for NP_001269546.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.
    Source sequence(s)
    AK055574, AK096595, AK126708, U73166, U73169
    Consensus CDS
    CCDS63644.1
    UniProtKB/TrEMBL
    B3KX51
    Related
    ENSP00000266027.6, ENST00000266027.9
    Conserved Domains (1) summary
    cd00066
    Location:1297
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
  3. NM_001282618.2NP_001269547.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4

    See identical proteins and their annotated locations for NP_001269547.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1.
    Source sequence(s)
    AK055574, AK096595, U73166
    UniProtKB/TrEMBL
    B3KP24
    Conserved Domains (1) summary
    cd00066
    Location:2268
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
  4. NM_001282619.2NP_001269548.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5

    See identical proteins and their annotated locations for NP_001269548.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AK055574, BC016995, U73166
    UniProtKB/TrEMBL
    B3KX51
    Conserved Domains (2) summary
    cd00066
    Location:24333
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
    smart00275
    Location:24337
    G_alpha; G protein alpha subunit
  5. NM_001282620.2NP_001269549.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6

    See identical proteins and their annotated locations for NP_001269549.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (6) has a shorter and distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AK055574, AK096595, U73166
    Consensus CDS
    CCDS63642.1
    UniProtKB/TrEMBL
    B3KX51
    Related
    ENSP00000406871.1, ENST00000422163.5
    Conserved Domains (1) summary
    cd00066
    Location:24333
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)
  6. NM_002070.4NP_002061.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1

    See identical proteins and their annotated locations for NP_002061.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AK055574, BC012138, U73166
    Consensus CDS
    CCDS2813.1
    UniProtKB/Swiss-Prot
    B3KTZ0, B4DYA0, B4E2X5, P04899, Q6B6N3, Q8IZ71
    UniProtKB/TrEMBL
    Q96C71
    Related
    ENSP00000312999.6, ENST00000313601.11
    Conserved Domains (1) summary
    cd00066
    Location:34349
    G-alpha; Alpha subunit of G proteins (guanine nucleotide binding)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    50227068..50263358
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047447979.1XP_047303935.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1

    UniProtKB/Swiss-Prot
    B3KTZ0, B4DYA0, B4E2X5, P04899, Q6B6N3, Q8IZ71

RNA

  1. XR_007095663.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    50256406..50288697
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054346201.1XP_054202176.1  guanine nucleotide-binding protein G(i) subunit alpha-2 isoform X1

    UniProtKB/Swiss-Prot
    B3KTZ0, B4DYA0, B4E2X5, P04899, Q6B6N3, Q8IZ71