Cpt1a carnitine palmitoyltransferase 1A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cpt1aprovided by RGD
Official Full Name: carnitine palmitoyltransferase 1Aprovided by RGD
Primary source: RGD:2396
See related: Ensembl:ENSRNOG00000014254 AllianceGenome:RGD:2396
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CPT-Ia
Summary: Enables carnitine O-palmitoyltransferase activity and identical protein binding activity. Involved in several processes, including fatty acid metabolic process; liver regeneration; and response to tetrachloromethane. Located in mitochondrial outer membrane. Is integral component of mitochondrial outer membrane. Biomarker of alcoholic hepatitis; obesity; and type 2 diabetes mellitus. Human ortholog(s) of this gene implicated in carnitine palmitoyltransferase I deficiency and lipid metabolism disorder. Orthologous to human CPT1A (carnitine palmitoyltransferase 1A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 466.6), Liver (RPKM 466.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q42

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (209993881..210056329)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (200564634..200627059)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (218568157..218629679)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CPT1a downregulation protects against 7-ketocholesterol-induced fibrosis in tubular epithelial cells by downregulating TGF-beta1, alpha- SMA and NLRP3.
Title: CPT1a downregulation protects against cholesterol-induced fibrosis in tubular epithelial cells by downregulating TGFβ-1 and inflammasome.
A significant up-regulation of Cpt1a, Acadl and Sirt3, all beta-oxidation genes, along with a significant deacetylation of the forkhead transcription factor 3a (FOXO3A) was found in alpha-LA-treated animals.
Title: Impact of dietary lipoic acid supplementation on liver mitochondrial bioenergetics and oxidative status on normally fed Wistar rats.
dietary fat elevates hepatic Cpt1a expression via a highly coordinated transcriptional mechanism involving increased H3K4Me2, reduced DNAMe, and recruitment of C/EBPbeta, PPARalpha, PGC1alpha, and BAF60a to the gene.
Title: Epigenetic regulation of carnitine palmitoyltransferase 1 (Cpt1a) by high fat diet.
Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats
Title: Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats.
Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype.
Title: Examination of carnitine palmitoyltransferase 1 abundance in white adipose tissue: implications in obesity research.
e found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements.
Title: Carnitine Palmitoyltransferase 1 Increases Lipolysis, UCP1 Protein Expression and Mitochondrial Activity in Brown Adipocytes.
Fructose feeding significantly decreased mRNA levels for PPAR alpha and CPT1A.
Title: High fructose consumption induces DNA methylation at PPARα and CPT1A promoter regions in the rat liver.
Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions.
Title: Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.
upregulation CPT1 expression leading to reduction in the accumulation of lipid intermediates in skeletal muscle.
Title: Mitofusion-2-mediated alleviation of insulin resistance in rats through reduction in lipid intermediate accumulation in skeletal muscle.
CPT-1 activity was altered in concert with fasting and refeeding states, supporting a physiological role of CPT-1a in mediating the control of feeding
Title: Important role of ventromedial hypothalamic carnitine palmitoyltransferase-1a in the control of food intake.

Submit: New GeneRIF Correction See all GeneRIFs (42)

General gene information

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Markers

AA960192 (e-PCR)
- UniSTS:160772

AU049995 (e-PCR)
- UniSTS:146437

RH128242 (e-PCR)
- UniSTS:211550

PMC21059P1 (e-PCR)
- UniSTS:272009

D1Got189 (e-PCR)
- UniSTS:113048

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables carnitine O-palmitoyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carnitine O-palmitoyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables carnitine O-palmitoyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables carnitine O-palmitoyltransferase activity	ISO Inferred from Sequence Orthology more info
enables carnitine O-palmitoyltransferase activity	TAS Traceable Author Statement more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables palmitoleoyltransferase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in aflatoxin metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in carnitine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in carnitine metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in carnitine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in carnitine metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in cellular response to fatty acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in eating behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in fatty acid beta-oxidation	IEA Inferred from Electronic Annotation more info
involved_in fatty acid beta-oxidation	IEP Inferred from Expression Pattern more info	PubMed
involved_in fatty acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fatty acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fatty acid metabolic process	IEP Inferred from Expression Pattern more info	PubMed
involved_in glucose metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in liver regeneration	IEP Inferred from Expression Pattern more info	PubMed
involved_in long-chain fatty acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in long-chain fatty acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-chain fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in long-chain fatty acid metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of fatty acid beta-oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of innate immune response	ISO Inferred from Sequence Orthology more info
involved_in regulation of fatty acid beta-oxidation	TAS Traceable Author Statement more info	PubMed
involved_in regulation of fatty acid oxidation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of lipid storage	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to alkaloid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient levels	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to tetrachloromethane	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in triglyceride metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
NOT located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: carnitine O-palmitoyltransferase 1, liver isoform

Names: CPT I; CPT1-L; CPTI-L; carnitine palmitoyltransferase 1, liver; carnitine palmitoyltransferase 1a, liver; succinyltransferase CPT1A

NP_113747.2

EC 2.3.1.21

XP_017444326.1

EC 2.3.1.21

XP_038958249.1

EC 2.3.1.21

XP_063138421.1

EC 2.3.1.21

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031559.2 → NP_113747.2 carnitine O-palmitoyltransferase 1, liver isoform

See identical proteins and their annotated locations for NP_113747.2

Status: VALIDATED

Source sequence(s)

AA875270, BC072522

UniProtKB/Swiss-Prot

P32198, P97780, Q6IMZ4

UniProtKB/TrEMBL

A0A8I5ZQ86, A6HYK3

Conserved Domains (2) summary

pfam00755
Location:171 → 761

Carn_acyltransf; Choline/Carnitine o-acyltransferase

pfam16484
Location:1 → 47

CPT_N; Carnitine O-palmitoyltransferase N-terminus

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

209993881..210056329

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039102321.2 → XP_038958249.1 carnitine O-palmitoyltransferase 1, liver isoform isoform X1

UniProtKB/Swiss-Prot

P32198, P97780, Q6IMZ4

UniProtKB/TrEMBL

A0A8I5ZQ86, A6HYK3

Conserved Domains (2) summary

pfam00755
Location:171 → 761

Carn_acyltransf; Choline/Carnitine o-acyltransferase

pfam16484
Location:1 → 47

CPT_N; Carnitine O-palmitoyltransferase N-terminus
XM_063282351.1 → XP_063138421.1 carnitine O-palmitoyltransferase 1, liver isoform isoform X1

UniProtKB/Swiss-Prot

P32198, P97780, Q6IMZ4

UniProtKB/TrEMBL

A0A8I5ZQ86, A6HYK3
XM_017588837.2 → XP_017444326.1 carnitine O-palmitoyltransferase 1, liver isoform isoform X1

UniProtKB/Swiss-Prot

P32198, P97780, Q6IMZ4

UniProtKB/TrEMBL

A0A8I5ZQ86, A6HYK3

Conserved Domains (2) summary

pfam00755
Location:171 → 761

Carn_acyltransf; Choline/Carnitine o-acyltransferase

pfam16484
Location:1 → 47

CPT_N; Carnitine O-palmitoyltransferase N-terminus