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Mcf2l MCF.2 cell line derived transforming sequence-like [ Rattus norvegicus (Norway rat) ]

Gene ID: 117020, updated on 2-Nov-2024

Summary

Symbol
Mcf2lprovided by RGD
Full Name
MCF.2 cell line derived transforming sequence-likeprovided by RGD
Primary source
RGD:619782
See related
EnsemblRapid:ENSRNOG00000028426 AllianceGenome:RGD:619782
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Ost; mcf 2l
Summary
Enables guanyl-nucleotide exchange factor activity. Involved in intracellular signal transduction. Located in cytoplasm. Orthologous to human MCF2L (MCF.2 cell line derived transforming sequence like). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in Brain (RPKM 86.9), Kidney (RPKM 46.2) and 8 other tissues See more
Orthologs
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Genomic context

See Mcf2l in Genome Data Viewer
Location:
16q12.5
Exon count:
38
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 16 NC_086034.1 (83209277..83355683, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 16 NC_051351.1 (76507133..76652893, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 16 NC_005115.4 (81839686..81986007, complement)

Chromosome 16 - NC_086034.1Genomic Context describing neighboring genes Neighboring gene small ribosomal subunit protein eS19-like Neighboring gene coagulation factor X Neighboring gene uncharacterized LOC134482421 Neighboring gene coagulation factor VII Neighboring gene ATPase phospholipid transporting 11A Neighboring gene uncharacterized LOC102548949 Neighboring gene uncharacterized LOC134482533

Genomic regions, transcripts, and products

Expression

  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

General gene information

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables 1-phosphatidylinositol binding ISO
Inferred from Sequence Orthology
more info
 
enables guanyl-nucleotide exchange factor activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables guanyl-nucleotide exchange factor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables guanyl-nucleotide exchange factor activity IEA
Inferred from Electronic Annotation
more info
 
enables guanyl-nucleotide exchange factor activity ISO
Inferred from Sequence Orthology
more info
 
enables phosphatidylinositol binding IEA
Inferred from Electronic Annotation
more info
 
enables phosphatidylinositol binding ISO
Inferred from Sequence Orthology
more info
 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 
located_in endomembrane system ISO
Inferred from Sequence Orthology
more info
 
located_in extrinsic component of cytoplasmic side of plasma membrane ISO
Inferred from Sequence Orthology
more info
 
is_active_in extrinsic component of membrane IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in lamellipodium ISO
Inferred from Sequence Orthology
more info
 
located_in membrane ISO
Inferred from Sequence Orthology
more info
 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
 

General protein information

Preferred Names
guanine nucleotide exchange factor DBS
Names
DBL's big sister
MCF2-transforming sequence-like protein
OST oncogene
mcf.2 transforming sequence-like

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001413769.1NP_001400698.1  guanine nucleotide exchange factor DBS isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000016
    Related
    ENSRNOP00000083374.2, ENSRNOT00000100402.2
  2. NM_053951.2NP_446403.2  guanine nucleotide exchange factor DBS isoform 1

    See identical proteins and their annotated locations for NP_446403.2

    Status: VALIDATED

    Source sequence(s)
    CH473970
    UniProtKB/TrEMBL
    A6IWK6, D4A7F3
    Conserved Domains (6) summary
    cd11857
    Location:10821134
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:46191
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:603780
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:322524
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:787918
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    pfam00169
    Location:801915
    PH; PH domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086034.1 Reference GRCr8

    Range
    83209277..83355683 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039094165.2XP_038950093.1  guanine nucleotide exchange factor DBS isoform X8

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Related
    ENSRNOP00000077897.2, ENSRNOT00000101752.2
    Conserved Domains (5) summary
    cd11857
    Location:11151167
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:76221
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:633810
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:352554
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:817948
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  2. XM_039094166.2XP_038950094.1  guanine nucleotide exchange factor DBS isoform X12

    UniProtKB/Swiss-Prot
    Q63406
    UniProtKB/TrEMBL
    D4A7F3
    Related
    ENSRNOP00000068993.2, ENSRNOT00000079096.3
    Conserved Domains (5) summary
    cd11857
    Location:10591111
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:76221
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:633810
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:352554
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:817948
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  3. XM_063275044.1XP_063131114.1  guanine nucleotide exchange factor DBS isoform X16

  4. XM_017599979.3XP_017455468.1  guanine nucleotide exchange factor DBS isoform X11

    UniProtKB/TrEMBL
    A0A8I5Y6G8
  5. XM_006253421.5XP_006253483.1  guanine nucleotide exchange factor DBS isoform X6

    UniProtKB/TrEMBL
    A0A8I5ZZZ7, D4A7F3
    Conserved Domains (6) summary
    cd11857
    Location:11281180
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    pfam00169
    Location:9001014
    PH; PH domain
  6. XM_039094163.2XP_038950091.1  guanine nucleotide exchange factor DBS isoform X7

    UniProtKB/TrEMBL
    D4A7F3
    Conserved Domains (5) summary
    cd11857
    Location:11281180
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  7. XM_039094162.2XP_038950090.1  guanine nucleotide exchange factor DBS isoform X4

    UniProtKB/TrEMBL
    A0A8I5Y6G8, A0A8I5ZWU6
    Conserved Domains (5) summary
    cd11857
    Location:11531205
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  8. XM_039094160.2XP_038950088.1  guanine nucleotide exchange factor DBS isoform X3

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Conserved Domains (5) summary
    cd11857
    Location:11811233
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  9. XM_006253416.5XP_006253478.1  guanine nucleotide exchange factor DBS isoform X1

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Related
    ENSRNOP00000085354.2, ENSRNOT00000111168.2
    Conserved Domains (6) summary
    cd11857
    Location:11841236
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    pfam00169
    Location:9001014
    PH; PH domain
  10. XM_039094159.2XP_038950087.1  guanine nucleotide exchange factor DBS isoform X2

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Conserved Domains (5) summary
    cd11857
    Location:11841236
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:145290
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:702879
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:421623
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:8861017
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  11. XM_006253423.5XP_006253485.1  guanine nucleotide exchange factor DBS isoform X9

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Conserved Domains (6) summary
    cd11857
    Location:10891141
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:50195
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:607784
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:326528
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:791922
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    pfam00169
    Location:805919
    PH; PH domain
  12. XM_039094167.2XP_038950095.1  guanine nucleotide exchange factor DBS isoform X13

    UniProtKB/TrEMBL
    A0A8L2QMZ3, D4A7F3
    Related
    ENSRNOP00000047024.6, ENSRNOT00000043170.8
    Conserved Domains (5) summary
    cd11857
    Location:10331085
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:50195
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:607784
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:326528
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:791922
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
  13. XM_063275040.1XP_063131110.1  guanine nucleotide exchange factor DBS isoform X5

  14. XM_006253424.5XP_006253486.1  guanine nucleotide exchange factor DBS isoform X10

    UniProtKB/TrEMBL
    A0A0G2KA20, A0A8I5Y6G8
    Related
    ENSRNOP00000075224.2, ENSRNOT00000087095.3
    Conserved Domains (6) summary
    cd11857
    Location:10831135
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    smart00516
    Location:44189
    SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
    cd00160
    Location:601778
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:320522
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:785916
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    pfam00169
    Location:799913
    PH; PH domain
  15. XM_063275042.1XP_063131112.1  guanine nucleotide exchange factor DBS isoform X15

  16. XM_063275041.1XP_063131111.1  guanine nucleotide exchange factor DBS isoform X14

  17. XM_039094169.2XP_038950097.1  guanine nucleotide exchange factor DBS isoform X17

    UniProtKB/TrEMBL
    A0A8I5Y6G8
    Conserved Domains (5) summary
    cd11857
    Location:926978
    SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
    cd00160
    Location:444621
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00176
    Location:163365
    SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
    cd01227
    Location:628759
    PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    cl15787
    Location:535
    SEC14; Sec14p-like lipid-binding domain. Found in secretory proteins, such as S. cerevisiae phosphatidylinositol transfer protein (Sec14p), and in lipid regulated proteins such as RhoGAPs, RhoGEFs and neurofibromin (NF1). SEC14 domain of Dbl is known to ...