ENGase85A Glycosyl hydrolase family 85 [Arabidopsis thaliana (thale cress)] - Gene

Summary

Official Symbol: ENGase85A
Official Full Name: Glycosyl hydrolase family 85
Primary source: TAIR:AT5G05460
Locus tag: AT5G05460
See related: Araport:AT5G05460
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: AtENGase85A; Endo-beta-N-acetyglucosaminidase 85A; K18I23.27; K18I23_27
Summary: Encodes a cytosolic beta-endo-N-acetyglucosaminidase (ENGase). ENGases N-glycans cleave the O-glycosidic linkage between the two GlcNAc residues of the N-glycan core structure and thus generate a protein with a single GlcNAc attached to asparagine.
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Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Data indicated that At5g05460 and At3g11040 were referred to as AtENGase85A and AtENGase85B respectively, reflecting their classification as Cazy family number 85 enzymes.
Title: The two endo-β-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels.
an Arabidopsis thaliana mutant in which the expression of two putative ENGase genes was suppressed
Title: Double-knockout of putative endo-β-N-acetylglucosaminidase (ENGase) genes in Arabidopsis thaliana: loss of ENGase activity induced accumulation of high-mannose type free N-glycans bearing N,N'-acetylchitobiosyl unit.

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NM_120628.5

Function	Evidence Code	Pubs
enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity	IDA Inferred from Direct Assay more info	PubMed
enables mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity	IGI Inferred from Genetic Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within N-glycan processing	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein deglycosylation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in vacuole	HDA more info	PubMed

NP_196165.3

Glycosyl hydrolase family 85; FUNCTIONS IN: hydrolase activity, acting on glycosyl bonds, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast, cytoplasm; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 85 (InterPro:IPR005201), Glycoside hydrolase, catalytic core (InterPro:IPR017853); BEST Arabidopsis thaliana protein match is: Glycosyl hydrolase family 85 (TAIR:AT3G11040.1); Has 480 Blast hits to 472 proteins in 209 species: Archae - 0; Bacteria - 262; Metazoa - 108; Fungi - 28; Plants - 47; Viruses - 0; Other Eukaryotes - 35 (source: NCBI BLink).

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