DREB2A DRE-binding protein 2A [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: DREB2A
Official Full Name: DRE-binding protein 2A
Primary source: TAIR:AT5G05410
Locus tag: AT5G05410
See related: Araport:AT5G05410
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2; DRE-binding protein 2A; DREB2; K18I23.22; K18I23_22
Summary: Encodes a transcription factor that specifically binds to DRE/CRT cis elements (responsive to drought and low-temperature stress). Belongs to the DREB subfamily A-2 of ERF/AP2 transcription factor family (DREB2A). There are eight members in this subfamily including DREB2B. The protein contains one AP2 domain. Overexpression of transcriptional activation domain of DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic Arabidopsis plants. Microarray and RNA gel blot analyses revealed that DREB2A regulates expression of many water stress#Aeiinducible genes.
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Genomic context

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See DREB2A in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 2

Sequence:: Chromosome: 5; NC_003076.8 (1602205..1604112)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These observations suggest that inhibition of NRD phosphorylation stabilizes and activates DREB2A in response to heat stress to enhance plant thermotolerance.
Title: Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana.
Genetic analysis indicated that the depletion of BTB/POZ AND MATH DOMAIN protein expression conferred enhanced thermotolerance via DREB2A stabilization
Title: BPM-CUL3 E3 ligase modulates thermotolerance by facilitating negative regulatory domain-mediated degradation of DREB2A in Arabidopsis.
NMR spectroscopy further demonstrated that DREB2A underwent coupled folding and binding with alpha-helix formation upon interaction with RCD1, whereas peptides from ANAC013 and ANAC046 formed different structures or were fuzzy in the complexes.
Title: Structures and Short Linear Motif of Disordered Transcription Factor Regions Provide Clues to the Interactome of the Cellular Hub Protein Radical-induced Cell Death1.
DPB3-1 associates with NF-YA and NF-YB subunits to enhance heat stress-inducible gene expression during heat stress responses in cooperation with DREB2A.
Title: Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.
transcriptional responses of the OPDA-responsive genes HsfA2 and DREB2A to the protein synthesis inhibitor cycloheximide and to the HSP90 inhibitor geldanamycin
Title: Mechanisms of induction of the stress-responsive transcription factors HsfA2 and DREB2A by 12-oxo-phytodienoic acid in Arabidopsis thaliana.
In this study, an attempt has been made to improve CT in drought hardy crop, peanut (Arachis hypogaea L., cv. TMV2) by co-expressing stress-responsive transcription factors (TFs), AtDREB2A, AtHB7 and AtABF3, associated with downstream gene expression
Title: Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7 and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L.).
The stabilization of DREB2A is important but not sufficient to induce target gene expression; further activation processes are required.
Title: Stabilization of Arabidopsis DREB2A is required but not sufficient for the induction of target genes under conditions of stress.
The stress-inducible expression of Arabidopsis thaliana DREB2A CA in transgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress.
Title: Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane.
GRF7 binds to a region of the DREB2A promoter and represses its activity.
Title: Arabidopsis growth-regulating factor7 functions as a transcriptional repressor of abscisic acid- and osmotic stress-responsive genes, including DREB2A.
Functional interactions between Dreb2a and Med25 induce conformational changes which in turn cause changes in their binding affinity.
Title: Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001036760.1

Supported by ESTs:

NM_120623.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription cis-regulatory region binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cellular response to hypoxia	HEP more info	PubMed
acts_upstream_of_or_within heat acclimation	IEP Inferred from Expression Pattern more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to UV-B	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to UV-B	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to heat	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to hydrogen peroxide	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to water deprivation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to water deprivation	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: DRE-binding protein 2A

NP_001031837.1

DRE-binding protein 2A (DREB2A); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: DRE/CRT-binding protein 2B (TAIR:AT3G11020.1); Has 5407 Blast hits to 5383 proteins in 247 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 5390; Viruses - 4; Other Eukaryotes - 11 (source: NCBI BLink).

NP_196160.1

DRE-binding protein 2A (DREB2A); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: DRE/CRT-binding protein 2B (TAIR:AT3G11020.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

1602205..1604112

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_120623.3 → NP_196160.1 DRE-binding protein 2A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_196160.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O82132, Q5Y4C5

UniProtKB/TrEMBL

A0A5S9Y271, A0A7G2FAJ0

Conserved Domains (1) summary

smart00380
Location:78 → 141

AP2; DNA-binding domain in plant proteins such as APETALA2 and EREBPs
NM_001036760.1 → NP_001031837.1 DRE-binding protein 2A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031837.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A5S9Y271, A8MSB1

Conserved Domains (1) summary

smart00380
Location:78 → 141

AP2; DNA-binding domain in plant proteins such as APETALA2 and EREBPs