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    IWS1 interacts with SUPT6H, CTD assembly factor 1 [ Homo sapiens (human) ]

    Gene ID: 55677, updated on 2-Nov-2024

    Summary

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    Official Symbol
    IWS1provided by HGNC
    Official Full Name
    interacts with SUPT6H, CTD assembly factor 1provided by HGNC
    Primary source
    HGNC:HGNC:25467
    See related
    Ensembl:ENSG00000163166 AllianceGenome:HGNC:25467
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    Involved in regulation of mRNA export from nucleus; regulation of mRNA processing; and transcription elongation-coupled chromatin remodeling. Located in nucleoplasm. [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in testis (RPKM 10.7), lymph node (RPKM 10.6) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See IWS1 in Genome Data Viewer
    Location:
    2q14.3
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (127480812..127527336, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (127916064..127962573, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (128238388..128284065, complement)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124907884 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128221175-128221782 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128237383-128238334 Neighboring gene RNA, U4 small nuclear 48, pseudogene Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128272241-128272823 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128283275-128283974 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16491 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16492 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:128284386-128284559 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16493 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128293291-128294141 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:128303085-128303585 Neighboring gene myosin VIIB Neighboring gene uncharacterized LOC105373609 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16494 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16495 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128335179-128335732 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16496 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16497 Neighboring gene uncharacterized LOC101927834

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation. Liu Z, et al. Biochem Biophys Res Commun, 2007 Feb 2. PMID 17184735
    2. A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS. Ling Y, et al. Nucleic Acids Res, 2006. PMID 16648364, Free PMC Article
    3. A ubiquitous disordered protein interaction module orchestrates transcription elongation. Cermakova K, et al. Science, 2021 Nov 26. PMID 34822292, Free PMC Article
    4. The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells. Gérard A, et al. Cell Host Microbe, 2015 Jan 14. PMID 25590759
    5. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Gao K, et al. Biochem Biophys Res Commun, 2015 Mar 20. PMID 25689719

    See all (70) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A ubiquitous disordered protein interaction module orchestrates transcription elongation.
      Title: A ubiquitous disordered protein interaction module orchestrates transcription elongation.
    2. common LEDGF/p75 interaction interface shared by JPO2, PogZ, MLL1, IWS1 and HIV IN
      Title: Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif.
    3. Altogether, these results indicate that a complex containing LEDGF/p75, Iws1, and Spt6 participates in regulating postintegration steps of HIV latency.
      Title: The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells.
    4. IWS1, an RNA processing regulator, is phosphorylated by Akt3 and Akt1 at Ser720/Thr721 in lung cancer
      Title: Phosphoproteomics screen reveals akt isoform-specific signals linking RNA processing to lung cancer.
    5. recombinant Spt6 binds selectively to a stretch of uninterrupted consensus repeats located in the N-terminal half of the CTD and recruits Iws1
      Title: The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.
    6. binding of Spt6 to Ser2-P RNAPII provides a cotranscriptional mechanism to recruit Iws1, REF1/Aly, and associated mRNA processing, surveillance, and export factors to responsive genes.
      Title: The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.
    7. Here we report that the human homolog of Iws1, hIws1, physically interacts with protein arginine methyltransferases PRMT5 which methylates elongation factor Spt5 and regulates its interaction with RNA polymerase II.
      Title: A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    EBI GWAS Catalog

    Description
    Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
    EBI GWAS Catalog

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of IWS1 by shRNA enhances HIV-1 replication in HIV-1-infected CD4+ T-cells and latently infected cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    integrase gag-pol The IN catalytic core domain has a higher binding affinity (Kd) for PSIP1 (LEDGF/p75) than the physiological binding partner, IWS1 PubMed
    gag-pol HIV-1 IN displaces IWS1 from PSIP1 (LEDGF/p75) efficiently PubMed
    gag-pol The HIV-1 IN interaction site on the PSIP1 (LEDGF/p75) integrase binding domain (IBD) overlaps with that of IWS1 PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ10006, FLJ14655, FLJ32319, MGC126375, MGC126376, DKFZp761G0123

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin remodeling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in poly(A)+ mRNA export from nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of mRNA export from nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of mRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in transcription elongation-coupled chromatin remodeling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    protein IWS1 homolog
    Names
    IWS1 homolog
    IWS1, SUPT6H interacting protein
    IWS1-like protein
    interacts with Spt6

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001410923.1 → NP_001397852.1  protein IWS1 homolog isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC010976
      Consensus CDS
      CCDS92859.1
      UniProtKB/TrEMBL
      A0A1B0GW95
      Related
      ENSP00000490836.2, ENST00000637187.2

    2. NM_017969.3 → NP_060439.2  protein IWS1 homolog isoform 1

      See identical proteins and their annotated locations for NP_060439.2

      Status: VALIDATED

      Source sequence(s)
      AI638093, BC110537, DA268882
      Consensus CDS
      CCDS2146.1
      UniProtKB/Swiss-Prot
      Q2TB65, Q6P157, Q8N3E8, Q96MI7, Q96ST2, Q9NV97, Q9NWH8
      UniProtKB/TrEMBL
      B4DL52
      Related
      ENSP00000295321.4, ENST00000295321.9
      Conserved Domains (2) summary
      pfam08618
      Location:228 → 332
      Opi1; Transcription factor Opi1
      cl00146
      Location:528 → 792
      TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      127480812..127527336 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_005263707.4 → XP_005263764.1  protein IWS1 homolog isoform X1

      UniProtKB/TrEMBL
      B4DL52
      Conserved Domains (2) summary
      pfam08618
      Location:233 → 337
      Opi1; Transcription factor Opi1
      cl00146
      Location:533 → 797
      TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

    2. XM_006712626.4 → XP_006712689.1  protein IWS1 homolog isoform X2

      UniProtKB/TrEMBL
      B4DL52
      Conserved Domains (2) summary
      pfam08618
      Location:233 → 337
      Opi1; Transcription factor Opi1
      cl00146
      Location:533 → 782
      TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

    3. XM_017004468.3 → XP_016859957.1  protein IWS1 homolog isoform X4

      Conserved Domains (2) summary
      pfam08618
      Location:21 → 125
      Opi1; Transcription factor Opi1
      cl00146
      Location:221 → 585
      TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

    4. XM_047444948.1 → XP_047300904.1  protein IWS1 homolog isoform X3

    5. XM_011511450.2 → XP_011509752.1  protein IWS1 homolog isoform X3

      UniProtKB/TrEMBL
      B4DL52
      Conserved Domains (2) summary
      pfam08618
      Location:233 → 337
      Opi1; Transcription factor Opi1
      cl00146
      Location:533 → 724
      TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

    6. XM_047444951.1 → XP_047300907.1  protein IWS1 homolog isoform X5

    RNA

    1. XR_001738828.2 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      127916064..127962573 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054342889.1 → XP_054198864.1  protein IWS1 homolog isoform X1

    2. XM_054342890.1 → XP_054198865.1  protein IWS1 homolog isoform X2

    3. XM_054342891.1 → XP_054198866.1  protein IWS1 homolog isoform X3

    4. XM_054342892.1 → XP_054198867.1  protein IWS1 homolog isoform X3

    5. XM_054342893.1 → XP_054198868.1  protein IWS1 homolog isoform X5

    RNA

    1. XR_008486446.1 RNA Sequence

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q96ST2.2 GenPept UniProtKB/Swiss-Prot:Q96ST2

    Gene LinkOut

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