NF-AT-Driven interleukin-4 transcription potentiated by NIP45

Science. 1996 Dec 13;274(5294):1903-5. doi: 10.1126/science.274.5294.1903.

Abstract

The induction of cytokine gene transcription is mediated in part by the nuclear factor of activated T cells (NF-AT). Factors involved in the mechanisms of NF-AT-mediated transcription are not well understood. A nuclear factor that interacted with the Rel homology domain (RHD) of NF-ATp was identified with the use of a two-hybrid interaction trap. Designated NIP45 (NF-AT interacting protein), it has minimal similarity to any known genes. Transcripts encoding this factor were enriched in lymphoid tissues and testes. NIP45 synergized with NF-ATp and the proto-oncogene c-Maf to activate the interleukin-4 (IL-4) cytokine promoter; transient overexpression of NIP45 with NF-ATp and c-maf in B lymphoma cells induced measurable endogenous IL-4 protein production. The identification of NIP45 advances our understanding of gene activation of cytokines, critical mediators of the immune response.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins / metabolism*
  • Genes, Reporter
  • Humans
  • Interleukin-4 / genetics*
  • Intracellular Signaling Peptides and Proteins*
  • Male
  • Molecular Sequence Data
  • NFATC Transcription Factors
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic
  • Proto-Oncogene Mas
  • Recombinant Fusion Proteins / metabolism
  • Spleen / metabolism
  • Testis / metabolism
  • Thymus Gland / metabolism
  • Transcription Factors / metabolism*
  • Transcriptional Activation*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • MAS1 protein, human
  • NFATC Transcription Factors
  • Nfatc2 protein, mouse
  • Nfatc2ip protein, mouse
  • Nuclear Proteins
  • Proto-Oncogene Mas
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Interleukin-4

Associated data

  • GENBANK/U76759