Evaluation of CMV Brite kit for detection of cytomegalovirus pp65 antigenemia in peripheral blood leukocytes by immunofluorescence

J Clin Microbiol. 1996 May;34(5):1337-9. doi: 10.1128/jcm.34.5.1337-1339.1996.

Abstract

The CMV Brite antigenemia kit was compared with culture and an established cytomegalovirus pp65 antigenemia assay (CMV AG). Of 300 clinical specimens tested, 92 were positive by CMV Brite, 83 were positive by CMV AG, and 34 were positive by culture. Discrepancies could be attributed to anticytomegalovirus therapy or low-level antigenemia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Viral / blood*
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / diagnosis*
  • Cytomegalovirus Infections / virology
  • Diagnostic Errors
  • Evaluation Studies as Topic
  • Fluorescent Antibody Technique / methods*
  • Humans
  • Phosphoproteins / blood
  • Phosphoproteins / immunology
  • Viral Matrix Proteins / blood
  • Viral Matrix Proteins / immunology
  • Viremia / diagnosis*
  • Viremia / virology
  • Virology / methods*

Substances

  • Antigens, Viral
  • Phosphoproteins
  • Viral Matrix Proteins
  • cytomegalovirus matrix protein 65kDa