Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels

A Bertl; J A Anderson; C L Slayman; H Sentenac; R F Gaber

doi:10.1007/BF02814074

Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels

Folia Microbiol (Praha). 1994;39(6):507-9. doi: 10.1007/BF02814074.

Authors

A Bertl¹, J A Anderson, C L Slayman, H Sentenac, R F Gaber

Affiliation

¹ Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06510, USA.

PMID: 8550001
DOI: 10.1007/BF02814074

Abstract

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arabidopsis
Arabidopsis Proteins*
Carrier Proteins / genetics
Cation Transport Proteins*
Fungal Proteins / genetics
Genes, Plant
Genetic Complementation Test
Homeodomain Proteins / genetics
Homeodomain Proteins / physiology
Ion Transport
Kinesins*
Membrane Proteins / genetics
Patch-Clamp Techniques
Plant Proteins / genetics
Plant Proteins / physiology*
Potassium / metabolism*
Potassium Channels / genetics
Potassium Channels / physiology*
Potassium Channels, Inwardly Rectifying*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins*
Sequence Deletion
Trans-Activators / genetics
Trans-Activators / physiology

Substances

ATK1 protein, Arabidopsis
Arabidopsis Proteins
Carrier Proteins
Cation Transport Proteins
Fungal Proteins
Homeodomain Proteins
KAT1 protein, Arabidopsis
Membrane Proteins
Plant Proteins
Potassium Channels
Potassium Channels, Inwardly Rectifying
Saccharomyces cerevisiae Proteins
TRK2 protein, S cerevisiae
Trans-Activators
TRK1 protein, S cerevisiae
Kinesins
Potassium