Characterization of the products of dinucleoside monophosphates d(GpN) irradiated in aqueous solutions

Radiat Res. 1993 Nov;136(2):171-7.

Abstract

The dinucleoside monophosphates d(GpT), d(GpC), and d(GpA) were X-irradiated in oxygenated aqueous solution. The principal products were identified in the intact modified dinucleoside monophosphates using NMR spectroscopy and FAB mass spectrometry. The 8-hydroxyguanine modification is a major product in each of the d(GpN). The d(GpN), where N is a pyrimidine nucleoside, also yield products in which the pyrimidine base is degraded to a formamido remnant. The most interesting product is one bearing two base damages, 8-hydroxylation of the guanine base and degradation of the pyrimidine base to a formamido remnant. This double lesion was observed in both d(GpN) where N is a pyrimidine base.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, High Pressure Liquid
  • DNA Damage
  • Dinucleoside Phosphates / radiation effects*
  • Magnetic Resonance Spectroscopy

Substances

  • Dinucleoside Phosphates