Abstract
The nucleoli of vertebrate cells contain a number of small RNAs that are generated by the processing of intron fragments of protein-coding gene transcripts. The host gene (UHG) for intro-encoded human U22 is unusual in that it specifies a polyadenylated but apparently noncoding RNA. Depletion of U22 from Xenopus oocytes by oligonucleotide-directed ribonuclease H targeting prevented the processing of 18S ribosomal RNA (rRNA) at both ends. The appearance of 18S rRNA was restored by injection of in vitro-synthesized U22 RNA. These results identify a cellular function for an intron-encoded small RNA.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Blotting, Northern
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Cell Nucleolus / chemistry*
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Humans
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Introns*
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Molecular Sequence Data
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Oligonucleotide Probes
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Oocytes / metabolism
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RNA Precursors / metabolism*
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RNA Processing, Post-Transcriptional
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RNA, Nuclear / chemistry
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RNA, Nuclear / genetics*
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RNA, Nuclear / physiology*
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RNA, Ribosomal, 18S / metabolism*
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RNA, Small Nuclear / chemistry
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RNA, Small Nuclear / genetics*
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RNA, Small Nuclear / physiology*
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Xenopus
Substances
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Oligonucleotide Probes
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RNA Precursors
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RNA, Nuclear
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RNA, Ribosomal, 18S
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RNA, Small Nuclear
Associated data
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GENBANK/L36586
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GENBANK/L36587
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GENBANK/L36588