Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4',6-diamidino-2-phenylindole fluorescence in nucleoid gradients

Anal Biochem. 1982 Apr;121(2):339-48. doi: 10.1016/0003-2697(82)90491-2.
No abstract available

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / analysis
  • Centrifugation, Density Gradient
  • DNA / analysis*
  • DNA, Superhelical / analysis*
  • Female
  • Fibroblasts / analysis
  • Fluorescent Dyes
  • Humans
  • Indoles*
  • Liver / metabolism
  • Nucleic Acid Conformation
  • Rats
  • Spectrometry, Fluorescence

Substances

  • DNA, Superhelical
  • Fluorescent Dyes
  • Indoles
  • DAPI
  • DNA